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Protein

Cucurbitadienol synthase

Gene

CPQ

Organism
Cucurbita pepo (Vegetable marrow) (Summer squash)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Oxidosqualene cyclase involved in the biosynthesis of the highly oxygenated tetracyclic triterpenes cucurbitacins. Converts oxidosqualene to cucurbitadienol and does not produce lanosterol.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = cucurbitadienol.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei265Proton acceptorSequence analysis1
Active sitei491Proton donorSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16525.
BRENDAi5.4.99.33. 1740.

Names & Taxonomyi

Protein namesi
Recommended name:
Cucurbitadienol synthase (EC:5.4.99.33)
Gene namesi
Name:CPQ
OrganismiCucurbita pepo (Vegetable marrow) (Summer squash)
Taxonomic identifieri3663 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi488L → P: No change of product-specificity. No change of product-specificity; when associated with V-489. 1
Mutagenesisi489I → V: No change of product-specificity. No change of product-specificity; when associated with P-488. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004129841 – 764Cucurbitadienol synthaseAdd BLAST764

Structurei

3D structure databases

ProteinModelPortaliQ6BE24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati104 – 146PFTB 1Add BLAST43
Repeati154 – 195PFTB 2Add BLAST42
Repeati520 – 565PFTB 3Add BLAST46
Repeati597 – 637PFTB 4Add BLAST41
Repeati646 – 687PFTB 5Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 5 PFTB repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6BE24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWRLKVGAES VGEEDEKWVK SVSNHLGRQV WEFCADAAAD TPHQLLQIQN
60 70 80 90 100
ARNHFHHNRF HRKQSSDLFL AIQYEKEIAK GAKGGAVKVK EGEEVGKEAV
110 120 130 140 150
KSTLERALGF YSAVQTRDGN WASDLGGPLF LLPGLVIALH VTGVLNSVLS
160 170 180 190 200
KHHRVEMCRY LYNHQNEDGG WGLHIEGTST MFGSALNYVA LRLLGEDADG
210 220 230 240 250
GDGGAMTKAR AWILERGGAT AITSWGKLWL SVLGVYEWSG NNPLPPEFWL
260 270 280 290 300
LPYSLPFHPG RMWCHCRMVY LPMSYLYGKR FVGPITPKVL SLRQELYTIP
310 320 330 340 350
YHEIDWNKSR NTCAKEDLYY PHPKMQDILW GSIYHVYEPL FTRWPGKRLR
360 370 380 390 400
EKALQAAMKH IHYEDENSRY ICLGPVNKVL NMLCCWVEDP YSDAFKLHLQ
410 420 430 440 450
RVHDYLWVAE DGMRMQGYNG SQLWDTAFSI QAIVATKLVD SYAPTLRKAH
460 470 480 490 500
DFVKDSQIQE DCPGDPNVWF RHIHKGAWPL STRDHGWLIS DCTAEGLKAS
510 520 530 540 550
LMLSKLPSTM VGEPLEKNRL CDAVNVLLSL QNDNGGFASY ELTRSYPWLE
560 570 580 590 600
LINPAETFGD IVIDYPYVEC TAATMEALTL FKKLHPGHRT KEIDTAIGKA
610 620 630 640 650
ANFLEKMQRA DGSWYGCWGV CFTYAGWFGI KGLVAAGRTY NSCLAIRKAC
660 670 680 690 700
EFLLSKELPG GGWGESYLSC QNKVYTNLEG NKPHLVNTAW VLMALIEAGQ
710 720 730 740 750
GERDPAPLHR AARLLMNSQL ENGDFVQQEI MGVFNKNCMI TYAAYRNIFP
760
IWALGEYCHR VLTE
Length:764
Mass (Da):86,517
Last modified:September 13, 2004 - v1
Checksum:i27C59E3B5A6BEC05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116238 mRNA. Translation: BAD34645.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116238 mRNA. Translation: BAD34645.1.

3D structure databases

ProteinModelPortaliQ6BE24.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16525.
BRENDAi5.4.99.33. 1740.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUCS_CUCPE
AccessioniPrimary (citable) accession number: Q6BE24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: September 13, 2004
Last modified: October 5, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.