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Protein

Transcription factor PU.1

Gene

Spi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi171 – 254ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PU.1
Gene namesi
Name:Spi1
Synonyms:Sfpi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1359607 Spi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041351 – 271Transcription factor PU.1Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141PhosphoserineBy similarity1
Modified residuei147PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6BDS1
PRIDEiQ6BDS1

PTM databases

iPTMnetiQ6BDS1
PhosphoSitePlusiQ6BDS1

Expressioni

Gene expression databases

BgeeiENSRNOG00000012172

Interactioni

Subunit structurei

Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity (By similarity). Interacts with CEBPE (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6BDS1, 1 interactor
STRINGi10116.ENSRNOP00000016306

Structurei

3D structure databases

ProteinModelPortaliQ6BDS1
SMRiQ6BDS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3805 Eukaryota
ENOG410XSXU LUCA
GeneTreeiENSGT00390000015212
HOGENOMiHOG000095520
HOVERGENiHBG002474
InParanoidiQ6BDS1
KOiK09438
OMAiPRMCLPY
OrthoDBiEOG091G0DTY
PhylomeDBiQ6BDS1
TreeFamiTF352494

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q6BDS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQACKMEGF PLVAPPSDEL VTYESELYQR QTHDYYSLIG GDGDSHSDHY
60 70 80 90 100
WDFSTHHVHS EFESFPENHF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM
110 120 130 140 150
APPHASLSHQ VSYMPRVCFP YPPLSPAHQQ SSDEEEGERQ SPPLEVSDGE
160 170 180 190 200
ADGLEPGPGL LHGETGSKKK IRLYQFLLDL LRSGDMKDSI WWVDKDKGTF
210 220 230 240 250
QFSSKHKEAL AHRWGIQKGN RKKMTYQKMA RALRNYGKTG EVKKVKKKLT
260 270
YQFSGEVLGR GGLAERRLPP H
Length:271
Mass (Da):31,089
Last modified:September 13, 2004 - v1
Checksum:iCDD9F21BAD449608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154364 mRNA Translation: BAD35169.1
BC091185 mRNA Translation: AAH91185.1
RefSeqiNP_001005892.1, NM_001005892.2
UniGeneiRn.230674

Genome annotation databases

EnsembliENSRNOT00000016306; ENSRNOP00000016306; ENSRNOG00000012172
GeneIDi366126
KEGGirno:366126
UCSCiRGD:1359607 rat

Similar proteinsi

Entry informationi

Entry nameiSPI1_RAT
AccessioniPrimary (citable) accession number: Q6BDS1
Secondary accession number(s): Q5BK69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health