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Protein

Transcription factor PU.1

Gene

Spi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi171 – 254ETSPROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PU.1
Gene namesi
Name:Spi1
Synonyms:Sfpi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1359607. Spi1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nuclear chromatin Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041351 – 271Transcription factor PU.1Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141PhosphoserineBy similarity1
Modified residuei147PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6BDS1.
PRIDEiQ6BDS1.

PTM databases

iPTMnetiQ6BDS1.
PhosphoSitePlusiQ6BDS1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012172.

Interactioni

Subunit structurei

Binds DNA as a monomer. Interacts with RUNX1, SPIB, CEBPD and NONO. Interacts with GFI1; the interaction represses SPI1 transcriptional activity (By similarity).By similarity

Protein-protein interaction databases

IntActiQ6BDS1. 1 interactor.
STRINGi10116.ENSRNOP00000016306.

Structurei

3D structure databases

ProteinModelPortaliQ6BDS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
GeneTreeiENSGT00390000015212.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiQ6BDS1.
KOiK09438.
OMAiPRMCLPY.
OrthoDBiEOG091G0DTY.
PhylomeDBiQ6BDS1.
TreeFamiTF352494.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6BDS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQACKMEGF PLVAPPSDEL VTYESELYQR QTHDYYSLIG GDGDSHSDHY
60 70 80 90 100
WDFSTHHVHS EFESFPENHF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM
110 120 130 140 150
APPHASLSHQ VSYMPRVCFP YPPLSPAHQQ SSDEEEGERQ SPPLEVSDGE
160 170 180 190 200
ADGLEPGPGL LHGETGSKKK IRLYQFLLDL LRSGDMKDSI WWVDKDKGTF
210 220 230 240 250
QFSSKHKEAL AHRWGIQKGN RKKMTYQKMA RALRNYGKTG EVKKVKKKLT
260 270
YQFSGEVLGR GGLAERRLPP H
Length:271
Mass (Da):31,089
Last modified:September 13, 2004 - v1
Checksum:iCDD9F21BAD449608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154364 mRNA. Translation: BAD35169.1.
BC091185 mRNA. Translation: AAH91185.1.
RefSeqiNP_001005892.1. NM_001005892.2.
UniGeneiRn.230674.

Genome annotation databases

EnsembliENSRNOT00000016306; ENSRNOP00000016306; ENSRNOG00000012172.
GeneIDi366126.
KEGGirno:366126.
UCSCiRGD:1359607. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154364 mRNA. Translation: BAD35169.1.
BC091185 mRNA. Translation: AAH91185.1.
RefSeqiNP_001005892.1. NM_001005892.2.
UniGeneiRn.230674.

3D structure databases

ProteinModelPortaliQ6BDS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6BDS1. 1 interactor.
STRINGi10116.ENSRNOP00000016306.

PTM databases

iPTMnetiQ6BDS1.
PhosphoSitePlusiQ6BDS1.

Proteomic databases

PaxDbiQ6BDS1.
PRIDEiQ6BDS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016306; ENSRNOP00000016306; ENSRNOG00000012172.
GeneIDi366126.
KEGGirno:366126.
UCSCiRGD:1359607. rat.

Organism-specific databases

CTDi6688.
RGDi1359607. Spi1.

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
GeneTreeiENSGT00390000015212.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiQ6BDS1.
KOiK09438.
OMAiPRMCLPY.
OrthoDBiEOG091G0DTY.
PhylomeDBiQ6BDS1.
TreeFamiTF352494.

Miscellaneous databases

PROiQ6BDS1.

Gene expression databases

BgeeiENSRNOG00000012172.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPI1_RAT
AccessioniPrimary (citable) accession number: Q6BDS1
Secondary accession number(s): Q5BK69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.