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Protein

PML-RARA-regulated adapter molecule 1

Gene

Pram1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in integrin signaling in neutrophils. Binds to PtdIns4P.1 Publication

GO - Molecular functioni

GO - Biological processi

  • integrin-mediated signaling pathway Source: MGI
  • regulation of neutrophil degranulation Source: MGI
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PML-RARA-regulated adapter molecule 1
Short name:
PRAM-1
Gene namesi
Name:Pram1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:3576625. Pram1.

Pathology & Biotechi

Disruption phenotypei

Mice are healthy and do not display any obvious abnormality. They have normal hematopoietic differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 675675PML-RARA-regulated adapter molecule 1PRO_0000270172Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei374 – 3741PhosphoserineCombined sources

Post-translational modificationi

May be phosphorylated on tyrosines.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6BCL1.
PaxDbiQ6BCL1.
PRIDEiQ6BCL1.

PTM databases

iPTMnetiQ6BCL1.
PhosphoSiteiQ6BCL1.

Expressioni

Tissue specificityi

Expressed in bone marrow and mature neutrophils. Weakly expressed in macrophages and mast cells.1 Publication

Gene expression databases

BgeeiQ6BCL1.
CleanExiMM_PRAM1.
ExpressionAtlasiQ6BCL1. baseline and differential.
GenevisibleiQ6BCL1. MM.

Interactioni

Subunit structurei

Interacts with SKAP2, LCP2 and DBNL. May interact with LYN (By similarity). Interacts with NEK6 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057065.

Structurei

3D structure databases

ProteinModelPortaliQ6BCL1.
SMRiQ6BCL1. Positions 577-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini581 – 65575SH3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi73 – 471399Pro-richAdd
BLAST

Domaini

The SH3 domain binds to PtdIns4P.By similarity

Sequence similaritiesi

Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410J0FT. Eukaryota.
ENOG410YKSE. LUCA.
GeneTreeiENSGT00530000063460.
HOGENOMiHOG000168515.
HOVERGENiHBG082165.
InParanoidiQ6BCL1.
KOiK19994.
OMAiPQPEFAD.
OrthoDBiEOG75QR3T.
TreeFamiTF337003.

Family and domain databases

InterProiIPR029294. hSH3.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14603. hSH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6BCL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSNQDFRNL QAKFQTSQPE LGELFRKTPK PELNKVLKKF PQTELSEQPK
60 70 80 90 100
KSSQSELSAV SLKPLQLQFA DLPRKPPQPG VLKKSPQPEF PHLANKPVQA
110 120 130 140 150
EFPRKPLHPE FTGLKKPSQA EFTDLKKPPQ PQFASLPKKP PKPEFGELSK
160 170 180 190 200
RPPQLETPQE PSAPPAQKLL KPEPNNPARP LGELKPKMFW HLEANEAPKR
210 220 230 240 250
PLPSESSTFP KKPLQPEAVV GFSRKSQPQS ESIEVSQTSP SKCGSRELDS
260 270 280 290 300
HSPQPDISTF PKNNENFRKP SYPQATGCPK SPKQPMFYEF PQTPPRKPES
310 320 330 340 350
CNPQSHSPLP DFNAFPKKHP QLQPSDLTRA SSEPEVCKVP KKTQKPDPNV
360 370 380 390 400
LSQKPSQPEL GHLPRTSSDP EFNSLPRKFL QPQHGKFFQP EFPKGLPRKP
410 420 430 440 450
KLPGSVSECS LPSASAGSSP QCPLSPGLIV PGIPRWRSED FQVQRPPRRR
460 470 480 490 500
PLPSASSLGH PPAKPALPPG PINIQSFRRA AATAAAVLKT GSSTGTHFQA
510 520 530 540 550
QQPQHIAQNP DEIYELYDAV EATDDSSISP RGRDEMLSTQ QATRWPQQEP
560 570 580 590 600
ELRKKATQPQ QLPATDPKLL KQIRKAEKAE REFRKKFKFE GEIVIHTKMM
610 620 630 640 650
IDPNAKTRRG GGKHLGIRRG EILEVIEFTS KDEMLCRDPK GKYGYVPRTA
660 670
LLPLETEVYD DVSFGDPLDM QPFPR
Length:675
Mass (Da):75,508
Last modified:July 27, 2011 - v2
Checksum:iCD5A2A14949B1732
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti537 – 5371L → P in AAT76049 (PubMed:15572693).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665714 mRNA. Translation: AAT76049.1.
CT033847 Genomic DNA. No translation available.
CCDSiCCDS37569.1.
RefSeqiNP_001002842.2. NM_001002842.2.
UniGeneiMm.272606.
Mm.408916.

Genome annotation databases

EnsembliENSMUST00000052079; ENSMUSP00000057065; ENSMUSG00000032739.
GeneIDi378460.
KEGGimmu:378460.
UCSCiuc008byz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665714 mRNA. Translation: AAT76049.1.
CT033847 Genomic DNA. No translation available.
CCDSiCCDS37569.1.
RefSeqiNP_001002842.2. NM_001002842.2.
UniGeneiMm.272606.
Mm.408916.

3D structure databases

ProteinModelPortaliQ6BCL1.
SMRiQ6BCL1. Positions 577-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057065.

PTM databases

iPTMnetiQ6BCL1.
PhosphoSiteiQ6BCL1.

Proteomic databases

MaxQBiQ6BCL1.
PaxDbiQ6BCL1.
PRIDEiQ6BCL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052079; ENSMUSP00000057065; ENSMUSG00000032739.
GeneIDi378460.
KEGGimmu:378460.
UCSCiuc008byz.1. mouse.

Organism-specific databases

CTDi84106.
MGIiMGI:3576625. Pram1.

Phylogenomic databases

eggNOGiENOG410J0FT. Eukaryota.
ENOG410YKSE. LUCA.
GeneTreeiENSGT00530000063460.
HOGENOMiHOG000168515.
HOVERGENiHBG082165.
InParanoidiQ6BCL1.
KOiK19994.
OMAiPQPEFAD.
OrthoDBiEOG75QR3T.
TreeFamiTF337003.

Miscellaneous databases

PROiQ6BCL1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6BCL1.
CleanExiMM_PRAM1.
ExpressionAtlasiQ6BCL1. baseline and differential.
GenevisibleiQ6BCL1. MM.

Family and domain databases

InterProiIPR029294. hSH3.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14603. hSH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiPRAM_MOUSE
AccessioniPrimary (citable) accession number: Q6BCL1
Secondary accession number(s): E9QL42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.