Q6BCD8 (Q6BCD8_9GAMM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malate dehydrogenase RuleBase RU000422 HAMAP MF_01516
EC=1.1.1.37 RuleBase RU000422 HAMAP MF_01516

Gene names

Name:

mdh HAMAP MF_01516 EMBL BAD30072.1

Organism

Moritella sp. 2C2 EMBL BAD30072.1

Taxonomic identifier

285041 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Moritellaceae › Moritella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. SAAS SAAS001252 HAMAP MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. RuleBase RU000422 SAAS SAAS023958 HAMAP MF_01516
Subunit structure	Homodimer By similarity. HAMAP MF_01516 SAAS SAAS023958
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family. HAMAP MF_01516 RuleBase RU004065

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle SAAS SAAS023958 RuleBase RU000422 HAMAP MF_01516
Ligand	NAD SAAS SAAS023958 RuleBase RU000422 HAMAP MF_01516
Molecular function	Oxidoreductase SAAS SAAS023958 HAMAP MF_01516 RuleBase RU004065 EMBL BAD30072.1
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity HAMAP MF_01516
	Nucleotide binding	117 – 119	3	NAD By similarity HAMAP MF_01516
Sites
	Active site	177	1	Proton acceptor By similarity HAMAP MF_01516
	Binding site	34	1	NAD By similarity HAMAP MF_01516
	Binding site	81	1	Substrate By similarity HAMAP MF_01516
	Binding site	87	1	Substrate By similarity HAMAP MF_01516
	Binding site	94	1	NAD By similarity HAMAP MF_01516
	Binding site	119	1	Substrate By similarity HAMAP MF_01516
	Binding site	153	1	Substrate By similarity HAMAP MF_01516
	Binding site	227	1	NAD By similarity HAMAP MF_01516

Sequences

	Sequence		Length	Mass (Da)
	Q6BCD8 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: DA8A42A7C8F5DB1B	FASTA	312	31,941
	10 20 30 40 50 60 MKVAVLGAAG GIGQALALLL KTQLPAGSEL SLYDIAPVTP GVAVDLSHIP TDVTITGFSG 70 80 90 100 110 120 IDPTAALVGA DVVLISAGVA RKPGMDRSDL FNINAGIIKN LASKCAEVCP TACIGIITNP 130 140 150 160 170 180 VNTTVPIAAE VLKQAGVYDK RKLFGITTLD VIRSETFVSA LKGISLADVA VPVIGGHSGA 190 200 210 220 230 240 TILPLLSQVK GVEFTAEEIA TLTTRIQNAG TEVVEAKAGG GSATLSMGHA AARFGLSLVR 250 260 270 280 290 300 ALQGEKGIVE CTYVDGGSEH ATFFAQPVLL GKNGVEEVLA YGDLSDFETN ARDAMLEELK 310 ANITLGEEFV AG

References

[1]

"Amino acid substitutions in malate dehydrogenases of piezophilic bacteria isolated from intestinal contents of deep-sea fishes retrieved from the abyssal zone." Saito R., Kato C., Nakayama A. J. Gen. Appl. Microbiol. 52:9-19(2006) [PubMed: 16598154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 2C2 EMBL BAD30072.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB185129 Genomic DNA. Translation: BAD30072.1.
3D structure databases
ProteinModelPortal	Q6BCD8.
SMR	Q6BCD8. Positions 1-310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01516. Malate_dehydrog_1. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q6BCD8_9GAMM

Accession

Primary (citable) accession number: Q6BCD8

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)