Q6B945 (FABH_GRATL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	Grc000008

Encoded on

Plastid; Chloroplast

Organism

Gracilaria tenuistipitata var. liui (Red alga)

Taxonomic identifier

285951 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Florideophyceae › Gracilariales › Gracilariaceae › Gracilaria

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Plastid › chloroplast HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Chloroplast Plastid
Molecular function	Acyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_0000110516
Regions
	Region	256 – 260	5	ACP-binding By similarity
Sites
	Active site	113	1	By similarity
	Active site	255	1	By similarity
	Active site	285	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6B945 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 4DF9347E04164614	FASTA	330	36,075
	10 20 30 40 50 60 MQGAQIISIG SAVPDLCINN KEISQIVETS DEWIVTRTGI QERRVLTGTN KSVVDLAYSA 70 80 90 100 110 120 AMKALDKIHM DPLEIDLIIL ATSSPNDLFG SASQVQAKIG AARAVAFDLT AACSGFVLAI 130 140 150 160 170 180 VTATQFIQNG SYKNILIIGA DVLSKWIDWS DRKTCILFGD GAGAAIIQAC YEEDNAILGF 190 200 210 220 230 240 QLNTDGKQYK KLSITYKENN YSLNTLKLFQ GQFQYISMNG KEVYKFAVSK VPASIIKCLN 250 260 270 280 290 300 ALHLSIQDVN WLLLHQANKR ILQAVANRLS VDNYKIISNL SKYGNTSAAS IPLALDEAWQ 310 320 330 NNQIAKEDII VISGFGAGLT WGTVVIKWKC

References

[1]

"Comparative analysis of the complete plastid genome sequence of the red alga Gracilaria tenuistipitata var. liui provides insights into the evolution of rhodoplasts and their relationship to other plastids." Hagopian J.C., Reis M., Kitajima J.P., Bhattacharya D., de Oliveira M.C. J. Mol. Evol. 59:464-477(2004) [PubMed: 15638458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY673996 Genomic DNA. Translation: AAT79590.1.
RefSeq	YP_063515.1. NC_006137.1.
3D structure databases
ProteinModelPortal	Q6B945.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2943979.
Phylogenomic databases
ProtClustDB	CHL00203.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_GRATL

Accession

Primary (citable) accession number: Q6B945

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	September 13, 2004
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)