Reviewed, UniProtKB/Swiss-Prot Q6B8U1 (ACSF_GRATL)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Synonyms:	ycf59
Ordered Locus Names:	Grc000113

Encoded on

Plastid; Chloroplast

Organism

Gracilaria tenuistipitata var. liui (Red alga)

Taxonomic identifier

285951 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Florideophyceae › Gracilariales › Gracilariaceae › Gracilaria

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_0000217543

Sequences

	Sequence		Length	Mass (Da)
	Q6B8U1-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: B383F9E9614DD7EE	FASTA	349	41,494
	10 20 30 40 50 60 MSTSQNLSQT PVKETLLTPR FYTTDFDEMS KLDISSNIDE FEALLQEFRA DYNRQHFIRD 70 80 90 100 110 120 QEFEQSWNNL DSGTKALFVE FLERSCTAEF SGFLLYKELS RKLDKSNPVI AECFLLMSRD 130 140 150 160 170 180 EARHAGFLNK AMSDFNLSLD LGFLTKSRKY TFFAPKFIFY ATYLSEKIGY WRYITIYRHL 190 200 210 220 230 240 EKHPEHRIYP IFKFFENWCQ DENRHGDFFA ALLKSQPQLL NNLEARLWCR FFLLSVFATM 250 260 270 280 290 300 YLNDFQRSNF YKSIGLDARQ YDMQVIRKTN ESASRIFPVA LNVDQPKFFQ YLDQCAVENK 310 320 330 340 KLIEIDQKYN NWFIKKLIKM PIYIKLCSCL IKLYLLPAIP SAHVVSTIR

References

[1]

"Comparative analysis of the complete plastid genome sequence of the red alga Gracilaria tenuistipitata var. liui provides insights into the evolution of rhodoplasts and their relationship to other plastids." Hagopian J.C., Reis M., Kitajima J.P., Bhattacharya D., de Oliveira M.C. J. Mol. Evol. 59:464-477(2004) [PubMed: 15638458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY673996 Genomic DNA. Translation: AAT79694.1.
RefSeq	YP_063619.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2943992.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_GRATL

Accession

Primary (citable) accession number: Q6B8U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	September 13, 2004
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)