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Q6B8I1 (DS13A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase 13 isoform A

Short name=DUSP13A
EC=3.1.3.16
EC=3.1.3.48
Alternative name(s):
Branching-enzyme interacting DSP
Muscle-restricted DSP
Short name=MDSP
Gene names
Name:DUSP13
Synonyms:BEDP, DUSP13A, MDSP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable protein tyrosine phosphatase. Has a phosphatase activity-independent regulatory role in MAP3K5/ASK1-mediated apoptosis, preventing MAP3K5/ASK1 inhibition by AKT1. Shows no phosphatase activity on MAPK1/ERK2, MAPK8/JNK, MAPK14/p38 and MAP3K5/ASK1. Ref.6

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Enzyme regulation

Inhibited by vanadate. Ref.1

Subunit structure

Interacts with MAP3K5/ASK1; may compete with AKT1 preventing MAP3K5/ASK1 phosphorylation by AKT1. Ref.6

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Skeletal muscle specific. Ref.1

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Biophysicochemical properties

Kinetic parameters:

KM=7.5 mM for p-nitrophenol phosphate (at pH 6.0) Ref.1

Sequence caution

The sequence AAH09778.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAF84580.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAF84586.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAG51843.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAG54592.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAG62362.1 differs from that shown. Reason: Erroneous termination at position 3. Translated as Glu.

The sequence BAG62362.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Alternative products

This entry describes 7 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]
Isoform 5 (identifier: Q6B8I1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform 1 (identifier: Q9UII6-1)

Also known as: TMDP;

The sequence of this isoform can be found in the external entry Q9UII6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative promoter usage.
Isoform 2 (identifier: Q6B8I1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     51-188: ATANNRFELW...DQQLRGAGQS → NSIKCAETGG
Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 4 (identifier: Q9UII6-4)

Also known as: TMDP-L2;

The sequence of this isoform can be found in the external entry Q9UII6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative promoter usage and alternative splicing.
Isoform 6 (identifier: Q6B8I1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     52-67: TANNRFELWKLGITHV → GPYSLPWGSATLPPWH
     68-188: Missing.
Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Isoform 7 (identifier: Q6B8I1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     51-92: ATANNRFELW...FYGSSVSYLG → RGPGTASLLV...SCGSWASPTC
     93-188: Missing.
Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Isoform 8 (identifier: Q6B8I1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     123-188: AKVLVHCVVG...DQQLRGAGQS → ELVLTPCHGA...DGGSPAWGRP
Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Dual specificity protein phosphatase 13 isoform A
PRO_0000381973

Regions

Domain37 – 183147Tyrosine-protein phosphatase

Sites

Active site1291Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence51 – 188138ATANN…GAGQS → NSIKCAETGG in isoform 2.
VSP_047819
Alternative sequence51 – 9242ATANN…VSYLG → RGPGTASLLVPWLGRHQVPF LPSPGPRQTTALSCGSWASP TC in isoform 7.
VSP_046445
Alternative sequence52 – 6716TANNR…GITHV → GPYSLPWGSATLPPWH in isoform 6.
VSP_046446
Alternative sequence68 – 188121Missing in isoform 6.
VSP_046447
Alternative sequence93 – 18896Missing in isoform 7.
VSP_046448
Alternative sequence123 – 18866AKVLV…GAGQS → ELVLTPCHGALPLCHPGTDP AGAAGGSGPGGHTEDGGSPA WGRP in isoform 8.
VSP_046449
Natural variant731K → R. Ref.2
Corresponds to variant rs7912300 [ dbSNP | Ensembl ].
VAR_058495

Experimental info

Mutagenesis971D → A: No effect on interaction with MAP3K5. Ref.6
Mutagenesis1291C → S: No effect on interaction with MAP3K5. Ref.6
Sequence conflict201S → G in AAH09778. Ref.5
Sequence conflict461F → S in BAG62362. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 5 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 88E604BD26E70EE5

FASTA18820,658
        10         20         30         40         50         60 
MAETSLPELG GEDKATPCPS ILELEELLRA GKSSCSRVDE VWPNLFIGDA ATANNRFELW 

        70         80         90        100        110        120 
KLGITHVLNA AHKGLYCQGG PDFYGSSVSY LGVPAHDLPD FDISAYFSSA ADFIHRALNT 

       130        140        150        160        170        180 
PGAKVLVHCV VGVSRSATLV LAYLMLHQRL SLRQAVITVR QHRWVFPNRG FLHQLCRLDQ 


QLRGAGQS 

« Hide

Isoform 1 (TMDP) [UniParc].

See Q9UII6.

Isoform 2 [UniParc].

Checksum: 83A12286F25BBE7C
Show »

FASTA606,337
Isoform 4 (TMDP-L2) [UniParc].

See Q9UII6.

Isoform 6 [UniParc].

Checksum: 5B6ED54212DA0242
Show »

FASTA677,195
Isoform 7 [UniParc].

Checksum: 4325575165B0055D
Show »

FASTA929,746
Isoform 8 [UniParc].

Checksum: 51BBCD025706F2A9
Show »

FASTA16617,283

References

« Hide 'large scale' references
[1]"Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2."
Chen H.-H., Luche R., Wei B., Tonks N.K.
J. Biol. Chem. 279:41404-41413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, ALTERNATIVE PROMOTER USAGE, ENZYME REGULATION, TISSUE SPECIFICITY.
[2]"Identification of novel dual-specificity protein phosphatase which interacts with glycogen-branching enzyme."
Skurat A.V., Dietrich A.D.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), VARIANT ARG-73.
Tissue: Skeletal muscle.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 6; 7 AND 8).
Tissue: Skeletal muscle.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Bone marrow.
[6]"Positive regulation of apoptosis signal-regulating kinase 1 by dual-specificity phosphatase 13A."
Park J.E., Park B.C., Kim H.A., Song M., Park S.G., Lee D.H., Kim H.J., Choi H.K., Kim J.T., Cho S.
Cell. Mol. Life Sci. 67:2619-2629(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH MAP3K5, MUTAGENESIS OF ASP-97 AND CYS-129.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY674051 mRNA. Translation: AAT79356.1.
AY040091 mRNA. Translation: AAK77966.1.
AK057012 mRNA. Translation: BAG51843.1. Sequence problems.
AK127886 mRNA. Translation: BAG54592.1. Sequence problems.
AK291891 mRNA. Translation: BAF84580.1. Sequence problems.
AK291897 mRNA. Translation: BAF84586.1. Sequence problems.
AK300679 mRNA. Translation: BAG62362.1. Sequence problems.
CH471083 Genomic DNA. Translation: EAW54564.1.
BC009778 mRNA. Translation: AAH09778.1. Sequence problems.
CCDSCCDS53542.1. [Q6B8I1-1]
RefSeqNP_001007272.1. NM_001007271.1. [Q6B8I1-1]
NP_001007273.1. NM_001007272.1.
NP_001007274.1. NM_001007273.1.
UniGeneHs.178170.

3D structure databases

ProteinModelPortalQ6B8I1.
SMRQ6B8I1. Positions 39-183.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119380. 1 interaction.
MINTMINT-1448408.

Polymorphism databases

DMDM74748394.

Proteomic databases

PRIDEQ6B8I1.

Protocols and materials databases

DNASU51207.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308475; ENSP00000311051; ENSG00000079393. [Q6B8I1-5]
ENST00000372702; ENSP00000361787; ENSG00000079393. [Q6B8I1-1]
ENST00000394707; ENSP00000452702; ENSG00000079393. [Q6B8I1-2]
ENST00000479884; ENSP00000474298; ENSG00000079393. [Q6B8I1-4]
ENST00000494588; ENSP00000474689; ENSG00000079393. [Q6B8I1-3]
GeneID51207.
KEGGhsa:51207.
UCSCuc001jws.3. human. [Q6B8I1-1]

Organism-specific databases

CTD51207.
GeneCardsGC10M076854.
HGNCHGNC:19681. DUSP13.
MIM613191. gene.
neXtProtNX_Q6B8I1.
PharmGKBPA134939640.
GenAtlasSearch...

Phylogenomic databases

KOK14165.
OMAAEATPCP.
PhylomeDBQ6B8I1.

Gene expression databases

ArrayExpressQ6B8I1.
BgeeQ6B8I1.
GenevestigatorQ6B8I1.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSPR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDUSP13.
GenomeRNAi51207.
NextBio54264.
PROQ6B8I1.
SOURCESearch...

Entry information

Entry nameDS13A_HUMAN
AccessionPrimary (citable) accession number: Q6B8I1
Secondary accession number(s): Q96J67
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 13, 2004
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM