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Q6B8I1

- DS13A_HUMAN

UniProt

Q6B8I1 - DS13A_HUMAN

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Protein

Dual specificity protein phosphatase 13 isoform A

Gene
DUSP13, BEDP, DUSP13A, MDSP
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable protein tyrosine phosphatase. Has a phosphatase activity-independent regulatory role in MAP3K5/ASK1-mediated apoptosis, preventing MAP3K5/ASK1 inhibition by AKT1. Shows no phosphatase activity on MAPK1/ERK2, MAPK8/JNK, MAPK14/p38 and MAP3K5/ASK1.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Enzyme regulationi

Inhibited by vanadate.1 Publication

Kineticsi

  1. KM=7.5 mM for p-nitrophenol phosphate (at pH 6.0)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 13 isoform A (EC:3.1.3.16, EC:3.1.3.48)
Short name:
DUSP13A
Alternative name(s):
Branching-enzyme interacting DSP
Muscle-restricted DSP
Short name:
MDSP
Gene namesi
Name:DUSP13
Synonyms:BEDP, DUSP13A, MDSP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:19681. DUSP13.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971D → A: No effect on interaction with MAP3K5. 1 Publication
Mutagenesisi129 – 1291C → S: No effect on interaction with MAP3K5. 1 Publication

Organism-specific databases

PharmGKBiPA134939640.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Dual specificity protein phosphatase 13 isoform APRO_0000381973Add
BLAST

Proteomic databases

PRIDEiQ6B8I1.

Expressioni

Tissue specificityi

Skeletal muscle specific.1 Publication

Gene expression databases

ArrayExpressiQ6B8I1.
BgeeiQ6B8I1.
GenevestigatoriQ6B8I1.

Interactioni

Subunit structurei

Interacts with MAP3K5/ASK1; may compete with AKT1 preventing MAP3K5/ASK1 phosphorylation by AKT1.1 Publication

Protein-protein interaction databases

BioGridi119380. 1 interaction.
MINTiMINT-1448408.

Structurei

3D structure databases

ProteinModelPortaliQ6B8I1.
SMRiQ6B8I1. Positions 39-183.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 183147Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

KOiK14165.
OMAiAEATPCP.
PhylomeDBiQ6B8I1.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative promoter usage and alternative splicing. Align

Isoform 5 (identifier: Q6B8I1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAETSLPELG GEDKATPCPS ILELEELLRA GKSSCSRVDE VWPNLFIGDA    50
ATANNRFELW KLGITHVLNA AHKGLYCQGG PDFYGSSVSY LGVPAHDLPD 100
FDISAYFSSA ADFIHRALNT PGAKVLVHCV VGVSRSATLV LAYLMLHQRL 150
SLRQAVITVR QHRWVFPNRG FLHQLCRLDQ QLRGAGQS 188

Note: Produced by alternative promoter usage.

Length:188
Mass (Da):20,658
Last modified:September 13, 2004 - v1
Checksum:i88E604BD26E70EE5
GO
Isoform 1 (identifier: Q9UII6-1) [UniParc]FASTAAdd to Basket

Also known as: TMDP

The sequence of this isoform can be found in the external entry Q9UII6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage.

Length:198
Mass (Da):22,149
GO
Isoform 2 (identifier: Q6B8I1-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-188: ATANNRFELW...DQQLRGAGQS → NSIKCAETGG

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:60
Mass (Da):6,337
Checksum:i83A12286F25BBE7C
GO
Isoform 4 (identifier: Q9UII6-4) [UniParc]FASTAAdd to Basket

Also known as: TMDP-L2

The sequence of this isoform can be found in the external entry Q9UII6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage and alternative splicing.

Length:248
Mass (Da):27,507
GO
Isoform 6 (identifier: Q6B8I1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-67: TANNRFELWKLGITHV → GPYSLPWGSATLPPWH
     68-188: Missing.

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Show »
Length:67
Mass (Da):7,195
Checksum:i5B6ED54212DA0242
GO
Isoform 7 (identifier: Q6B8I1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-92: ATANNRFELW...FYGSSVSYLG → RGPGTASLLV...SCGSWASPTC
     93-188: Missing.

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Show »
Length:92
Mass (Da):9,746
Checksum:i4325575165B0055D
GO
Isoform 8 (identifier: Q6B8I1-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-188: AKVLVHCVVG...DQQLRGAGQS → ELVLTPCHGA...DGGSPAWGRP

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Show »
Length:166
Mass (Da):17,283
Checksum:i51BBCD025706F2A9
GO

Sequence cautioni

The sequence BAG62362.1 differs from that shown. Reason: Erroneous termination at position 3. Translated as Glu.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti73 – 731K → R.1 Publication
Corresponds to variant rs7912300 [ dbSNP | Ensembl ].
VAR_058495

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei51 – 188138ATANN…GAGQS → NSIKCAETGG in isoform 2. VSP_047819Add
BLAST
Alternative sequencei51 – 9242ATANN…VSYLG → RGPGTASLLVPWLGRHQVPF LPSPGPRQTTALSCGSWASP TC in isoform 7. VSP_046445Add
BLAST
Alternative sequencei52 – 6716TANNR…GITHV → GPYSLPWGSATLPPWH in isoform 6. VSP_046446Add
BLAST
Alternative sequencei68 – 188121Missing in isoform 6. VSP_046447Add
BLAST
Alternative sequencei93 – 18896Missing in isoform 7. VSP_046448Add
BLAST
Alternative sequencei123 – 18866AKVLV…GAGQS → ELVLTPCHGALPLCHPGTDP AGAAGGSGPGGHTEDGGSPA WGRP in isoform 8. VSP_046449Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201S → G in AAH09778. 1 Publication
Sequence conflicti46 – 461F → S in BAG62362. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY674051 mRNA. Translation: AAT79356.1.
AY040091 mRNA. Translation: AAK77966.1.
AK057012 mRNA. Translation: BAG51843.1. Sequence problems.
AK127886 mRNA. Translation: BAG54592.1. Sequence problems.
AK291891 mRNA. Translation: BAF84580.1. Sequence problems.
AK291897 mRNA. Translation: BAF84586.1. Sequence problems.
AK300679 mRNA. Translation: BAG62362.1. Sequence problems.
CH471083 Genomic DNA. Translation: EAW54564.1.
BC009778 mRNA. Translation: AAH09778.1. Sequence problems.
CCDSiCCDS53542.1. [Q6B8I1-1]
RefSeqiNP_001007272.1. NM_001007271.1. [Q6B8I1-1]
NP_001007273.1. NM_001007272.1.
NP_001007274.1. NM_001007273.1.
UniGeneiHs.178170.

Genome annotation databases

EnsembliENST00000308475; ENSP00000311051; ENSG00000079393. [Q6B8I1-5]
ENST00000372702; ENSP00000361787; ENSG00000079393. [Q6B8I1-1]
ENST00000394707; ENSP00000452702; ENSG00000079393. [Q6B8I1-2]
ENST00000479884; ENSP00000474298; ENSG00000079393. [Q6B8I1-4]
ENST00000494588; ENSP00000474689; ENSG00000079393. [Q6B8I1-3]
GeneIDi51207.
KEGGihsa:51207.
UCSCiuc001jws.3. human. [Q6B8I1-1]

Polymorphism databases

DMDMi74748394.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY674051 mRNA. Translation: AAT79356.1 .
AY040091 mRNA. Translation: AAK77966.1 .
AK057012 mRNA. Translation: BAG51843.1 . Sequence problems.
AK127886 mRNA. Translation: BAG54592.1 . Sequence problems.
AK291891 mRNA. Translation: BAF84580.1 . Sequence problems.
AK291897 mRNA. Translation: BAF84586.1 . Sequence problems.
AK300679 mRNA. Translation: BAG62362.1 . Sequence problems.
CH471083 Genomic DNA. Translation: EAW54564.1 .
BC009778 mRNA. Translation: AAH09778.1 . Sequence problems.
CCDSi CCDS53542.1. [Q6B8I1-1 ]
RefSeqi NP_001007272.1. NM_001007271.1. [Q6B8I1-1 ]
NP_001007273.1. NM_001007272.1.
NP_001007274.1. NM_001007273.1.
UniGenei Hs.178170.

3D structure databases

ProteinModelPortali Q6B8I1.
SMRi Q6B8I1. Positions 39-183.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119380. 1 interaction.
MINTi MINT-1448408.

Polymorphism databases

DMDMi 74748394.

Proteomic databases

PRIDEi Q6B8I1.

Protocols and materials databases

DNASUi 51207.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308475 ; ENSP00000311051 ; ENSG00000079393 . [Q6B8I1-5 ]
ENST00000372702 ; ENSP00000361787 ; ENSG00000079393 . [Q6B8I1-1 ]
ENST00000394707 ; ENSP00000452702 ; ENSG00000079393 . [Q6B8I1-2 ]
ENST00000479884 ; ENSP00000474298 ; ENSG00000079393 . [Q6B8I1-4 ]
ENST00000494588 ; ENSP00000474689 ; ENSG00000079393 . [Q6B8I1-3 ]
GeneIDi 51207.
KEGGi hsa:51207.
UCSCi uc001jws.3. human. [Q6B8I1-1 ]

Organism-specific databases

CTDi 51207.
GeneCardsi GC10M076854.
HGNCi HGNC:19681. DUSP13.
MIMi 613191. gene.
neXtProti NX_Q6B8I1.
PharmGKBi PA134939640.
GenAtlasi Search...

Phylogenomic databases

KOi K14165.
OMAi AEATPCP.
PhylomeDBi Q6B8I1.

Miscellaneous databases

GeneWikii DUSP13.
GenomeRNAii 51207.
NextBioi 54264.
PROi Q6B8I1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6B8I1.
Bgeei Q6B8I1.
Genevestigatori Q6B8I1.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF00782. DSPc. 1 hit.
[Graphical view ]
PRINTSi PR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2."
    Chen H.-H., Luche R., Wei B., Tonks N.K.
    J. Biol. Chem. 279:41404-41413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, ALTERNATIVE PROMOTER USAGE, ENZYME REGULATION, TISSUE SPECIFICITY.
  2. "Identification of novel dual-specificity protein phosphatase which interacts with glycogen-branching enzyme."
    Skurat A.V., Dietrich A.D.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), VARIANT ARG-73.
    Tissue: Skeletal muscle.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 6; 7 AND 8).
    Tissue: Skeletal muscle.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Bone marrow.
  6. "Positive regulation of apoptosis signal-regulating kinase 1 by dual-specificity phosphatase 13A."
    Park J.E., Park B.C., Kim H.A., Song M., Park S.G., Lee D.H., Kim H.J., Choi H.K., Kim J.T., Cho S.
    Cell. Mol. Life Sci. 67:2619-2629(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH MAP3K5, MUTAGENESIS OF ASP-97 AND CYS-129.

Entry informationi

Entry nameiDS13A_HUMAN
AccessioniPrimary (citable) accession number: Q6B8I1
Secondary accession number(s): Q96J67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 13, 2004
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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