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Protein

Dual specificity protein phosphatase 13 isoform A

Gene

DUSP13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable protein tyrosine phosphatase. Has a phosphatase activity-independent regulatory role in MAP3K5/ASK1-mediated apoptosis, preventing MAP3K5/ASK1 inhibition by AKT1. Shows no phosphatase activity on MAPK1/ERK2, MAPK8/JNK, MAPK14/p38 and MAP3K5/ASK1.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Enzyme regulationi

Inhibited by vanadate.1 Publication

Kineticsi

  1. KM=7.5 mM for p-nitrophenol phosphate (at pH 6.0)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei129Phosphocysteine intermediatePROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    • dephosphorylation Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 13 isoform A (EC:3.1.3.16, EC:3.1.3.48)
    Short name:
    DUSP13A
    Alternative name(s):
    Branching-enzyme interacting DSP
    Muscle-restricted DSP
    Short name:
    MDSP
    Gene namesi
    Name:DUSP13
    Synonyms:BEDP, DUSP13A, MDSP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 10

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000079393.20
    HGNCiHGNC:19681 DUSP13
    MIMi613191 gene
    neXtProtiNX_Q6B8I1

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi97D → A: No effect on interaction with MAP3K5. 1 Publication1
    Mutagenesisi129C → S: No effect on interaction with MAP3K5. 1 Publication1

    Organism-specific databases

    DisGeNETi51207
    OpenTargetsiENSG00000079393
    PharmGKBiPA134939640

    Polymorphism and mutation databases

    BioMutaiGPR143
    DMDMi74748394

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003819731 – 188Dual specificity protein phosphatase 13 isoform AAdd BLAST188

    Proteomic databases

    PeptideAtlasiQ6B8I1
    PRIDEiQ6B8I1

    PTM databases

    DEPODiQ6B8I1

    Expressioni

    Tissue specificityi

    Skeletal muscle specific.1 Publication

    Gene expression databases

    BgeeiENSG00000079393
    ExpressionAtlasiQ6B8I1 baseline and differential
    GenevisibleiQ6B8I1 HS

    Interactioni

    Subunit structurei

    Interacts with MAP3K5/ASK1; may compete with AKT1 preventing MAP3K5/ASK1 phosphorylation by AKT1.1 Publication

    Protein-protein interaction databases

    BioGridi119380, 78 interactors

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5XJVX-ray1.69A/B1-185[»]
    ProteinModelPortaliQ6B8I1
    SMRiQ6B8I1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini37 – 183Tyrosine-protein phosphataseAdd BLAST147

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00760000118853
    KOiK14165
    OMAiSHVDEVW
    PhylomeDBiQ6B8I1

    Family and domain databases

    CDDicd00127 DSPc, 1 hit
    Gene3Di3.90.190.10, 1 hit
    InterProiView protein in InterPro
    IPR020417 Atypical_DUSP
    IPR020405 Atypical_DUSP_famA
    IPR000340 Dual-sp_phosphatase_cat-dom
    IPR024950 DUSP
    IPR029021 Prot-tyrosine_phosphatase-like
    IPR016130 Tyr_Pase_AS
    IPR003595 Tyr_Pase_cat
    IPR000387 TYR_PHOSPHATASE_dom
    IPR020422 TYR_PHOSPHATASE_DUAL_dom
    PANTHERiPTHR10159 PTHR10159, 1 hit
    PfamiView protein in Pfam
    PF00782 DSPc, 1 hit
    PRINTSiPR01908 ADSPHPHTASE
    PR01909 ADSPHPHTASEA
    SMARTiView protein in SMART
    SM00195 DSPc, 1 hit
    SM00404 PTPc_motif, 1 hit
    SUPFAMiSSF52799 SSF52799, 1 hit
    PROSITEiView protein in PROSITE
    PS00383 TYR_PHOSPHATASE_1, 1 hit
    PS50056 TYR_PHOSPHATASE_2, 1 hit
    PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

    Sequences (7)i

    Sequence statusi: Complete.

    This entry describes 7 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

    Note: Additional isoforms seem to exist.
    Isoform 5 (identifier: Q6B8I1-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAETSLPELG GEDKATPCPS ILELEELLRA GKSSCSRVDE VWPNLFIGDA
    60 70 80 90 100
    ATANNRFELW KLGITHVLNA AHKGLYCQGG PDFYGSSVSY LGVPAHDLPD
    110 120 130 140 150
    FDISAYFSSA ADFIHRALNT PGAKVLVHCV VGVSRSATLV LAYLMLHQRL
    160 170 180
    SLRQAVITVR QHRWVFPNRG FLHQLCRLDQ QLRGAGQS
    Note: Produced by alternative promoter usage.
    Length:188
    Mass (Da):20,658
    Last modified:September 13, 2004 - v1
    Checksum:i88E604BD26E70EE5
    GO
    Isoform 1 (identifier: Q9UII6-1) [UniParc]FASTAAdd to basket
    Also known as: TMDP
    The sequence of this isoform can be found in the external entry Q9UII6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Note: Produced by alternative promoter usage.
    Length:198
    Mass (Da):22,149
    GO
    Isoform 2 (identifier: Q6B8I1-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         51-188: ATANNRFELW...DQQLRGAGQS → NSIKCAETGG

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:60
    Mass (Da):6,337
    Checksum:i83A12286F25BBE7C
    GO
    Isoform 4 (identifier: Q9UII6-4) [UniParc]FASTAAdd to basket
    Also known as: TMDP-L2
    The sequence of this isoform can be found in the external entry Q9UII6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Note: Produced by alternative promoter usage and alternative splicing.
    Length:248
    Mass (Da):27,507
    GO
    Isoform 6 (identifier: Q6B8I1-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         52-67: TANNRFELWKLGITHV → GPYSLPWGSATLPPWH
         68-188: Missing.

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
    Show »
    Length:67
    Mass (Da):7,195
    Checksum:i5B6ED54212DA0242
    GO
    Isoform 7 (identifier: Q6B8I1-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         51-92: ATANNRFELW...FYGSSVSYLG → RGPGTASLLV...SCGSWASPTC
         93-188: Missing.

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
    Show »
    Length:92
    Mass (Da):9,746
    Checksum:i4325575165B0055D
    GO
    Isoform 8 (identifier: Q6B8I1-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         123-188: AKVLVHCVVG...DQQLRGAGQS → ELVLTPCHGA...DGGSPAWGRP

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
    Show »
    Length:166
    Mass (Da):17,283
    Checksum:i51BBCD025706F2A9
    GO

    Sequence cautioni

    The sequence AAH09778 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
    The sequence BAF84580 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
    The sequence BAF84586 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
    The sequence BAG51843 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
    The sequence BAG54592 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
    The sequence BAG62362 differs from that shown. Reason: Erroneous termination at position 3. Translated as Glu.Curated
    The sequence BAG62362 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti20S → G in AAH09778 (PubMed:15489334).Curated1
    Sequence conflicti46F → S in BAG62362 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_05849573K → R1 PublicationCorresponds to variant dbSNP:rs7912300Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_04781951 – 188ATANN…GAGQS → NSIKCAETGG in isoform 2. 1 PublicationAdd BLAST138
    Alternative sequenceiVSP_04644551 – 92ATANN…VSYLG → RGPGTASLLVPWLGRHQVPF LPSPGPRQTTALSCGSWASP TC in isoform 7. 1 PublicationAdd BLAST42
    Alternative sequenceiVSP_04644652 – 67TANNR…GITHV → GPYSLPWGSATLPPWH in isoform 6. 1 PublicationAdd BLAST16
    Alternative sequenceiVSP_04644768 – 188Missing in isoform 6. 1 PublicationAdd BLAST121
    Alternative sequenceiVSP_04644893 – 188Missing in isoform 7. 1 PublicationAdd BLAST96
    Alternative sequenceiVSP_046449123 – 188AKVLV…GAGQS → ELVLTPCHGALPLCHPGTDP AGAAGGSGPGGHTEDGGSPA WGRP in isoform 8. 1 PublicationAdd BLAST66

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY674051 mRNA Translation: AAT79356.1
    AY040091 mRNA Translation: AAK77966.1
    AK057012 mRNA Translation: BAG51843.1 Sequence problems.
    AK127886 mRNA Translation: BAG54592.1 Sequence problems.
    AK291891 mRNA Translation: BAF84580.1 Sequence problems.
    AK291897 mRNA Translation: BAF84586.1 Sequence problems.
    AK300679 mRNA Translation: BAG62362.1 Sequence problems.
    CH471083 Genomic DNA Translation: EAW54564.1
    BC009778 mRNA Translation: AAH09778.1 Sequence problems.
    CCDSiCCDS53542.1 [Q6B8I1-1]
    RefSeqiNP_001007272.1, NM_001007271.1 [Q6B8I1-1]
    NP_001007273.1, NM_001007272.1
    NP_001007274.1, NM_001007273.1
    NP_001307771.1, NM_001320842.1
    NP_001307772.1, NM_001320843.1
    UniGeneiHs.178170

    Genome annotation databases

    EnsembliENST00000308475; ENSP00000311051; ENSG00000079393 [Q6B8I1-5]
    ENST00000372702; ENSP00000361787; ENSG00000079393 [Q6B8I1-1]
    ENST00000394707; ENSP00000452702; ENSG00000079393 [Q6B8I1-2]
    ENST00000479884; ENSP00000474298; ENSG00000079393 [Q6B8I1-4]
    ENST00000494588; ENSP00000474689; ENSG00000079393 [Q6B8I1-3]
    GeneIDi51207
    KEGGihsa:51207
    UCSCiuc001jws.4 human [Q6B8I1-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiDS13A_HUMAN
    AccessioniPrimary (citable) accession number: Q6B8I1
    Secondary accession number(s): Q96J67
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: September 13, 2004
    Last modified: May 23, 2018
    This is version 112 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
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    Main funding by: National Institutes of Health