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Q6B8I1

- DS13A_HUMAN

UniProt

Q6B8I1 - DS13A_HUMAN

Protein

Dual specificity protein phosphatase 13 isoform A

Gene

DUSP13

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Probable protein tyrosine phosphatase. Has a phosphatase activity-independent regulatory role in MAP3K5/ASK1-mediated apoptosis, preventing MAP3K5/ASK1 inhibition by AKT1. Shows no phosphatase activity on MAPK1/ERK2, MAPK8/JNK, MAPK14/p38 and MAP3K5/ASK1.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Enzyme regulationi

    Inhibited by vanadate.1 Publication

    Kineticsi

    1. KM=7.5 mM for p-nitrophenol phosphate (at pH 6.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei129 – 1291Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
    2. protein tyrosine phosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 13 isoform A (EC:3.1.3.16, EC:3.1.3.48)
    Short name:
    DUSP13A
    Alternative name(s):
    Branching-enzyme interacting DSP
    Muscle-restricted DSP
    Short name:
    MDSP
    Gene namesi
    Name:DUSP13
    Synonyms:BEDP, DUSP13A, MDSP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:19681. DUSP13.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi97 – 971D → A: No effect on interaction with MAP3K5. 1 Publication
    Mutagenesisi129 – 1291C → S: No effect on interaction with MAP3K5. 1 Publication

    Organism-specific databases

    PharmGKBiPA134939640.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Dual specificity protein phosphatase 13 isoform APRO_0000381973Add
    BLAST

    Proteomic databases

    PRIDEiQ6B8I1.

    Expressioni

    Tissue specificityi

    Skeletal muscle specific.1 Publication

    Gene expression databases

    ArrayExpressiQ6B8I1.
    BgeeiQ6B8I1.
    GenevestigatoriQ6B8I1.

    Interactioni

    Subunit structurei

    Interacts with MAP3K5/ASK1; may compete with AKT1 preventing MAP3K5/ASK1 phosphorylation by AKT1.1 Publication

    Protein-protein interaction databases

    BioGridi119380. 1 interaction.
    MINTiMINT-1448408.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6B8I1.
    SMRiQ6B8I1. Positions 39-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 183147Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    KOiK14165.
    OMAiAEATPCP.
    PhylomeDBiQ6B8I1.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR020417. Atypical_DUSP.
    IPR020405. Atypical_DUSP_famA.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    PRINTSiPR01908. ADSPHPHTASE.
    PR01909. ADSPHPHTASEA.
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequences (7)i

    Sequence statusi: Complete.

    This entry describes 7 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Isoform 5 (identifier: Q6B8I1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAETSLPELG GEDKATPCPS ILELEELLRA GKSSCSRVDE VWPNLFIGDA    50
    ATANNRFELW KLGITHVLNA AHKGLYCQGG PDFYGSSVSY LGVPAHDLPD 100
    FDISAYFSSA ADFIHRALNT PGAKVLVHCV VGVSRSATLV LAYLMLHQRL 150
    SLRQAVITVR QHRWVFPNRG FLHQLCRLDQ QLRGAGQS 188

    Note: Produced by alternative promoter usage.

    Length:188
    Mass (Da):20,658
    Last modified:September 13, 2004 - v1
    Checksum:i88E604BD26E70EE5
    GO
    Isoform 1 (identifier: Q9UII6-1) [UniParc]FASTAAdd to Basket

    Also known as: TMDP

    The sequence of this isoform can be found in the external entry Q9UII6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative promoter usage.

    Length:198
    Mass (Da):22,149
    GO
    Isoform 2 (identifier: Q6B8I1-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         51-188: ATANNRFELW...DQQLRGAGQS → NSIKCAETGG

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:60
    Mass (Da):6,337
    Checksum:i83A12286F25BBE7C
    GO
    Isoform 4 (identifier: Q9UII6-4) [UniParc]FASTAAdd to Basket

    Also known as: TMDP-L2

    The sequence of this isoform can be found in the external entry Q9UII6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative promoter usage and alternative splicing.

    Length:248
    Mass (Da):27,507
    GO
    Isoform 6 (identifier: Q6B8I1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         52-67: TANNRFELWKLGITHV → GPYSLPWGSATLPPWH
         68-188: Missing.

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:67
    Mass (Da):7,195
    Checksum:i5B6ED54212DA0242
    GO
    Isoform 7 (identifier: Q6B8I1-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         51-92: ATANNRFELW...FYGSSVSYLG → RGPGTASLLV...SCGSWASPTC
         93-188: Missing.

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:92
    Mass (Da):9,746
    Checksum:i4325575165B0055D
    GO
    Isoform 8 (identifier: Q6B8I1-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         123-188: AKVLVHCVVG...DQQLRGAGQS → ELVLTPCHGA...DGGSPAWGRP

    Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:166
    Mass (Da):17,283
    Checksum:i51BBCD025706F2A9
    GO

    Sequence cautioni

    The sequence BAG62362.1 differs from that shown. Reason: Erroneous termination at position 3. Translated as Glu.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201S → G in AAH09778. (PubMed:15489334)Curated
    Sequence conflicti46 – 461F → S in BAG62362. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti73 – 731K → R.1 Publication
    Corresponds to variant rs7912300 [ dbSNP | Ensembl ].
    VAR_058495

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei51 – 188138ATANN…GAGQS → NSIKCAETGG in isoform 2. 1 PublicationVSP_047819Add
    BLAST
    Alternative sequencei51 – 9242ATANN…VSYLG → RGPGTASLLVPWLGRHQVPF LPSPGPRQTTALSCGSWASP TC in isoform 7. 1 PublicationVSP_046445Add
    BLAST
    Alternative sequencei52 – 6716TANNR…GITHV → GPYSLPWGSATLPPWH in isoform 6. 1 PublicationVSP_046446Add
    BLAST
    Alternative sequencei68 – 188121Missing in isoform 6. 1 PublicationVSP_046447Add
    BLAST
    Alternative sequencei93 – 18896Missing in isoform 7. 1 PublicationVSP_046448Add
    BLAST
    Alternative sequencei123 – 18866AKVLV…GAGQS → ELVLTPCHGALPLCHPGTDP AGAAGGSGPGGHTEDGGSPA WGRP in isoform 8. 1 PublicationVSP_046449Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY674051 mRNA. Translation: AAT79356.1.
    AY040091 mRNA. Translation: AAK77966.1.
    AK057012 mRNA. Translation: BAG51843.1. Sequence problems.
    AK127886 mRNA. Translation: BAG54592.1. Sequence problems.
    AK291891 mRNA. Translation: BAF84580.1. Sequence problems.
    AK291897 mRNA. Translation: BAF84586.1. Sequence problems.
    AK300679 mRNA. Translation: BAG62362.1. Sequence problems.
    CH471083 Genomic DNA. Translation: EAW54564.1.
    BC009778 mRNA. Translation: AAH09778.1. Sequence problems.
    CCDSiCCDS53542.1. [Q6B8I1-1]
    RefSeqiNP_001007272.1. NM_001007271.1. [Q6B8I1-1]
    NP_001007273.1. NM_001007272.1.
    NP_001007274.1. NM_001007273.1.
    UniGeneiHs.178170.

    Genome annotation databases

    EnsembliENST00000308475; ENSP00000311051; ENSG00000079393. [Q6B8I1-5]
    ENST00000372702; ENSP00000361787; ENSG00000079393. [Q6B8I1-1]
    ENST00000394707; ENSP00000452702; ENSG00000079393. [Q6B8I1-2]
    ENST00000479884; ENSP00000474298; ENSG00000079393. [Q6B8I1-4]
    ENST00000494588; ENSP00000474689; ENSG00000079393. [Q6B8I1-3]
    GeneIDi51207.
    KEGGihsa:51207.
    UCSCiuc001jws.3. human. [Q6B8I1-1]

    Polymorphism databases

    DMDMi74748394.

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY674051 mRNA. Translation: AAT79356.1 .
    AY040091 mRNA. Translation: AAK77966.1 .
    AK057012 mRNA. Translation: BAG51843.1 . Sequence problems.
    AK127886 mRNA. Translation: BAG54592.1 . Sequence problems.
    AK291891 mRNA. Translation: BAF84580.1 . Sequence problems.
    AK291897 mRNA. Translation: BAF84586.1 . Sequence problems.
    AK300679 mRNA. Translation: BAG62362.1 . Sequence problems.
    CH471083 Genomic DNA. Translation: EAW54564.1 .
    BC009778 mRNA. Translation: AAH09778.1 . Sequence problems.
    CCDSi CCDS53542.1. [Q6B8I1-1 ]
    RefSeqi NP_001007272.1. NM_001007271.1. [Q6B8I1-1 ]
    NP_001007273.1. NM_001007272.1.
    NP_001007274.1. NM_001007273.1.
    UniGenei Hs.178170.

    3D structure databases

    ProteinModelPortali Q6B8I1.
    SMRi Q6B8I1. Positions 39-183.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119380. 1 interaction.
    MINTi MINT-1448408.

    Polymorphism databases

    DMDMi 74748394.

    Proteomic databases

    PRIDEi Q6B8I1.

    Protocols and materials databases

    DNASUi 51207.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308475 ; ENSP00000311051 ; ENSG00000079393 . [Q6B8I1-5 ]
    ENST00000372702 ; ENSP00000361787 ; ENSG00000079393 . [Q6B8I1-1 ]
    ENST00000394707 ; ENSP00000452702 ; ENSG00000079393 . [Q6B8I1-2 ]
    ENST00000479884 ; ENSP00000474298 ; ENSG00000079393 . [Q6B8I1-4 ]
    ENST00000494588 ; ENSP00000474689 ; ENSG00000079393 . [Q6B8I1-3 ]
    GeneIDi 51207.
    KEGGi hsa:51207.
    UCSCi uc001jws.3. human. [Q6B8I1-1 ]

    Organism-specific databases

    CTDi 51207.
    GeneCardsi GC10M076854.
    HGNCi HGNC:19681. DUSP13.
    MIMi 613191. gene.
    neXtProti NX_Q6B8I1.
    PharmGKBi PA134939640.
    GenAtlasi Search...

    Phylogenomic databases

    KOi K14165.
    OMAi AEATPCP.
    PhylomeDBi Q6B8I1.

    Miscellaneous databases

    GeneWikii DUSP13.
    GenomeRNAii 51207.
    NextBioi 54264.
    PROi Q6B8I1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6B8I1.
    Bgeei Q6B8I1.
    Genevestigatori Q6B8I1.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR020417. Atypical_DUSP.
    IPR020405. Atypical_DUSP_famA.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    PRINTSi PR01908. ADSPHPHTASE.
    PR01909. ADSPHPHTASEA.
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2."
      Chen H.-H., Luche R., Wei B., Tonks N.K.
      J. Biol. Chem. 279:41404-41413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, ALTERNATIVE PROMOTER USAGE, ENZYME REGULATION, TISSUE SPECIFICITY.
    2. "Identification of novel dual-specificity protein phosphatase which interacts with glycogen-branching enzyme."
      Skurat A.V., Dietrich A.D.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), VARIANT ARG-73.
      Tissue: Skeletal muscle.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 6; 7 AND 8).
      Tissue: Skeletal muscle.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Bone marrow.
    6. "Positive regulation of apoptosis signal-regulating kinase 1 by dual-specificity phosphatase 13A."
      Park J.E., Park B.C., Kim H.A., Song M., Park S.G., Lee D.H., Kim H.J., Choi H.K., Kim J.T., Cho S.
      Cell. Mol. Life Sci. 67:2619-2629(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH MAP3K5, MUTAGENESIS OF ASP-97 AND CYS-129.

    Entry informationi

    Entry nameiDS13A_HUMAN
    AccessioniPrimary (citable) accession number: Q6B8I1
    Secondary accession number(s): Q96J67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3