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Protein

Sphingosine kinase B

Gene

sgkB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), which probably acts intracellularly as a second messenger perhaps by promoting cell proliferation.

Catalytic activityi

ATP + sphinganine = ADP + sphinganine 1-phosphate.
ATP + sphingosine = ADP + sphingosine 1-phosphate.

Enzyme regulationi

Inhibited by N,N,-dimethylsphingosine.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei288 – 2881ATPPROSITE-ProRule annotation
Active sitei315 – 3151Proton donor/acceptorBy similarity
Binding sitei320 – 3201ATPPROSITE-ProRule annotation
Binding sitei461 – 4611SubstrateBy similarity
Binding sitei468 – 4681ATPPROSITE-ProRule annotation
Binding sitei474 – 4741ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi257 – 2593ATPPROSITE-ProRule annotation
Nucleotide bindingi345 – 3473ATPPROSITE-ProRule annotation
Nucleotide bindingi734 – 7363ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: dictyBase
  • D-erythro-sphingosine kinase activity Source: dictyBase
  • sphinganine kinase activity Source: UniProtKB-EC

GO - Biological processi

  • dihydrosphingosine-1-P pathway Source: dictyBase
  • positive regulation of asexual reproduction Source: dictyBase
  • sphingolipid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-1660661. Sphingolipid de novo biosynthesis.
R-DDI-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-DDI-5218921. VEGFR2 mediated cell proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine kinase B (EC:2.7.1.91)
Short name:
SK B
Short name:
SPK B
Gene namesi
Name:sgkB
Synonyms:SK, SPHK
ORF Names:DDB_G0284545
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284545. sgkB.

Pathology & Biotechi

Disruption phenotypei

Cells grow more slowly on solid media and are more sensitive than wild-type to the antitumor agents cisplatin and carboplatin; both effects are exacerbated in sgkA and sgkB double knockout. Exogenous S1P reverses the sensitivity to cisplatin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 760760Sphingosine kinase BPRO_0000328251Add
BLAST

Proteomic databases

PaxDbiQ6B516.
PRIDEiQ6B516.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0220131.

Structurei

3D structure databases

ProteinModelPortaliQ6B516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 383137DAGKcPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 3164Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 538Poly-Asn
Compositional biasi91 – 10313Poly-AsnAdd
BLAST
Compositional biasi178 – 18811Poly-ThrAdd
BLAST
Compositional biasi394 – 4029Poly-Thr
Compositional biasi415 – 44228Poly-AsnAdd
BLAST
Compositional biasi556 – 5627Poly-Asn
Compositional biasi563 – 5686Poly-Thr
Compositional biasi582 – 5876Poly-Thr

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
InParanoidiQ6B516.
KOiK04718.
OMAiMEPEHAK.
PhylomeDBiQ6B516.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6B516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNNEPAE TVQEKGPKLK NDIDLNDQFK DEKEKKEEIS SSSIENKNNN
60 70 80 90 100
NNNTSTNNVI PNESNNNISN NNVISNENNN TNNNTDNNTD NNNNNNNNNN
110 120 130 140 150
NNNEPVTSFT VNNKSLIEKE PTLFEFNQEQ QIKYKNKSIN SLIIKRSGIV
160 170 180 190 200
IKISQKKSTI IYQDSIIACS ELPDNFDTAT TTTTTTTTLP IKVTLFTCVL
210 220 230 240 250
KKKQLTIDKE QRKRKSYTFQ FKSNQDSLNF YSNIQSTFLN SLPRGNPKNR
260 270 280 290 300
KIRILINPKS GKKESHNIFK EVEQLFKDSG IKMKLTVTME PEHAKKIGFK
310 320 330 340 350
SNIYKYDTVV FISGDGLLHE FINGLLSRED YEDAKKIPLA LIPAGTGNGL
360 370 380 390 400
ANSIGLQDPM SAALAILRGF TKPLDVCIVQ QPTVTTIPVV DNNTVTTTTT
410 420 430 440 450
TTSPTSASPT ITSANNNNNN NNNNNNNNNN NNNNNNNNNN SNITKWCSIL
460 470 480 490 500
SLTWGLVSDV DIESEKYRSL GDLRLIIGAA VRILNLRIYR GKVYFLPAIP
510 520 530 540 550
LDKSQMQSIP KCSFDCNICD SSNSVKVIED LVCNDNDNNN KNKNEEQNEI
560 570 580 590 600
NSTTSNNNNN NNTTTTSTSS STSTSTSTSS LTATTTTAKS TNSLSSSPRS
610 620 630 640 650
DINMSSNSIS KSLDIGTIPS CKVTHNSNLL NESSDSLLSK GWKCIEGEFI
660 670 680 690 700
GVVASTVSHL ASDFISSPNA HLSDGLIDLI FINNRSKLSK ASLLSILTDS
710 720 730 740 750
ATGDHLKSDL IEHHKVKALI LEPSIQKHGI VAIDGERIPY AKTSMENIRG
760
CLNLICRSYH
Length:760
Mass (Da):83,966
Last modified:September 13, 2004 - v1
Checksum:iD505C2167ACB266C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY679519 mRNA. Translation: AAT80892.1.
AAFI02000066 Genomic DNA. Translation: EAL65199.1.
RefSeqiXP_638540.1. XM_633448.1.

Genome annotation databases

EnsemblProtistsiDDB0220131; DDB0220131; DDB_G0284545.
GeneIDi8624633.
KEGGiddi:DDB_G0284545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY679519 mRNA. Translation: AAT80892.1.
AAFI02000066 Genomic DNA. Translation: EAL65199.1.
RefSeqiXP_638540.1. XM_633448.1.

3D structure databases

ProteinModelPortaliQ6B516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0220131.

Proteomic databases

PaxDbiQ6B516.
PRIDEiQ6B516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0220131; DDB0220131; DDB_G0284545.
GeneIDi8624633.
KEGGiddi:DDB_G0284545.

Organism-specific databases

dictyBaseiDDB_G0284545. sgkB.

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
InParanoidiQ6B516.
KOiK04718.
OMAiMEPEHAK.
PhylomeDBiQ6B516.

Enzyme and pathway databases

ReactomeiR-DDI-1660661. Sphingolipid de novo biosynthesis.
R-DDI-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-DDI-5218921. VEGFR2 mediated cell proliferation.

Miscellaneous databases

PROiQ6B516.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPHKB_DICDI
AccessioniPrimary (citable) accession number: Q6B516
Secondary accession number(s): Q54PJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: December 9, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.