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Protein

Partitioning defective 6 homolog alpha

Gene

Pard6a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog alpha
Short name:
PAR-6
Short name:
PAR-6 alpha
Short name:
PAR-6A
Gene namesi
Name:Pard6a
Synonyms:Par-6a, Par6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303273. Pard6a.

Subcellular locationi

GO - Cellular componenti

  • axonal growth cone Source: RGD
  • bicellular tight junction Source: UniProtKB-SubCell
  • centriolar satellite Source: UniProtKB
  • centrosome Source: UniProtKB
  • neuronal cell body Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125151 – 346Partitioning defective 6 homolog alphaAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineCombined sources1
Modified residuei345PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by the TGF-beta receptor.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6B4M5.

PTM databases

iPTMnetiQ6B4M5.
PhosphoSitePlusiQ6B4M5.

Interactioni

Subunit structurei

Interacts with MPP5 and CRB3. Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with MAP2K5. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with DCTN1 and PCM1.By similarity

Protein-protein interaction databases

BioGridi258812. 1 interactor.
DIPiDIP-46160N.
IntActiQ6B4M5. 1 interactor.
STRINGi10116.ENSRNOP00000043456.

Structurei

3D structure databases

ProteinModelPortaliQ6B4M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 95PB1PROSITE-ProRule annotationAdd BLAST81
Domaini133 – 150Pseudo-CRIBAdd BLAST18
Domaini157 – 250PDZPROSITE-ProRule annotationAdd BLAST94

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116Interaction with PRKCI and PRKCZBy similarityAdd BLAST116
Regioni126 – 253Interaction with PARD3 and CDC42By similarityAdd BLAST128

Domaini

The PB1 domain mediates interactions with MAP2K5.By similarity
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.By similarity
The PDZ domain mediates interaction with CRB3.By similarity

Sequence similaritiesi

Belongs to the PAR6 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 pseudo-CRIB domain.Curated

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ6B4M5.
PhylomeDBiQ6B4M5.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6B4M5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRTSVRGFQ EFSRLLCVVH
60 70 80 90 100
QIPGLDVLLG YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEGDS
110 120 130 140 150
SGLAFASNSL QRRKKGLLLR PVAPLRTRPP LLISLPQDFR QVSSVIDVDL
160 170 180 190 200
LPETHRRVRL HKHGSDRPLG FYIRDGMSVR VAPQGLERVP GIFISRLVRG
210 220 230 240 250
GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH NLIVTVKPAN
260 270 280 290 300
QRNNVVRGAS GRLTGPSSVG PGPTDPDSDD DNSDPVIENR HPPCSNGLSQ
310 320 330 340
GPLCWDLQPG CLHPSAGSSL PSLDSREQAN SGWGNGMRGD VSGFSL
Length:346
Mass (Da):37,398
Last modified:September 27, 2005 - v2
Checksum:iE363D7F8960DEF7F
GO
Isoform 2 (identifier: Q6B4M5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Show »
Length:345
Mass (Da):37,327
Checksum:i4EF8C104CD084E0B
GO
Isoform 3 (identifier: Q6B4M5-3) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.

Show »
Length:170
Mass (Da):17,679
Checksum:iD51CE009212982E9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0157681 – 176Missing in isoform 3. 1 PublicationAdd BLAST176
Alternative sequenceiVSP_01576996Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY682586 mRNA. Translation: AAT80897.1.
AY682587 mRNA. Translation: AAT80898.1.
DQ011518 mRNA. Translation: AAY32917.1.
UniGeneiRn.16053.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY682586 mRNA. Translation: AAT80897.1.
AY682587 mRNA. Translation: AAT80898.1.
DQ011518 mRNA. Translation: AAY32917.1.
UniGeneiRn.16053.

3D structure databases

ProteinModelPortaliQ6B4M5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi258812. 1 interactor.
DIPiDIP-46160N.
IntActiQ6B4M5. 1 interactor.
STRINGi10116.ENSRNOP00000043456.

PTM databases

iPTMnetiQ6B4M5.
PhosphoSitePlusiQ6B4M5.

Proteomic databases

PaxDbiQ6B4M5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi1303273. Pard6a.

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ6B4M5.
PhylomeDBiQ6B4M5.

Miscellaneous databases

PROiQ6B4M5.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR6A_RAT
AccessioniPrimary (citable) accession number: Q6B4M5
Secondary accession number(s): Q4U4X0, Q6B4M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.