ID   TDA8_YEAST              Reviewed;         126 AA.
AC   Q6B2U8; D6VPF3;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   24-JAN-2024, entry version 104.
DE   RecName: Full=Topoisomerase I damage affected protein 8;
GN   Name=TDA8; OrderedLocusNames=YAL064C-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA   Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA   Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA   Storms R.K.;
RT   "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=21173034; DOI=10.1101/gr.109033.110;
RA   Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S.,
RA   Wagner M., Rothstein R.;
RT   "Selective ploidy ablation, a high-throughput plasmid transfer protocol,
RT   identifies new genes affecting topoisomerase I-induced DNA damage.";
RL   Genome Res. 21:477-486(2011).
CC   -!- DISRUPTION PHENOTYPE: Leads to cell death when overexpressing the
CC       camptothecin mimetic TOP1-T(722)A mutant.
CC       {ECO:0000269|PubMed:21173034}.
CC   -!- SIMILARITY: Belongs to the flocculin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U12980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AY692632; AAT92651.1; -; Genomic_DNA.
DR   EMBL; BK006935; DAA06923.1; -; Genomic_DNA.
DR   RefSeq; NP_058136.1; NM_001180041.1.
DR   AlphaFoldDB; Q6B2U8; -.
DR   SMR; Q6B2U8; -.
DR   BioGRID; 31765; 39.
DR   STRING; 4932.YAL064C-A; -.
DR   PaxDb; 4932-YAL064C-A; -.
DR   EnsemblFungi; YAL064C-A_mRNA; YAL064C-A; YAL064C-A.
DR   GeneID; 851234; -.
DR   KEGG; sce:YAL064C-A; -.
DR   AGR; SGD:S000002140; -.
DR   SGD; S000002140; TDA8.
DR   VEuPathDB; FungiDB:YAL064C-A; -.
DR   GeneTree; ENSGT00940000180962; -.
DR   HOGENOM; CLU_1983318_0_0_1; -.
DR   InParanoid; Q6B2U8; -.
DR   OrthoDB; 2039698at2759; -.
DR   BioCyc; YEAST:G3O-28892-MONOMER; -.
DR   BioGRID-ORCS; 851234; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q6B2U8; -.
DR   Proteomes; UP000002311; Chromosome I.
DR   RNAct; Q6B2U8; Protein.
DR   Gene3D; 2.60.120.1560; -; 1.
DR   InterPro; IPR037524; PA14/GLEYA.
DR   InterPro; IPR011658; PA14_dom.
DR   Pfam; PF07691; PA14; 1.
DR   PROSITE; PS51820; PA14; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..126
FT                   /note="Topoisomerase I damage affected protein 8"
FT                   /id="PRO_0000268622"
FT   DOMAIN          1..110
FT                   /note="PA14"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01164"
SQ   SEQUENCE   126 AA;  13886 MW;  7EA7549C4C0618D7 CRC64;
     MTGYFLPPQT SSYTFRFAKV DDSAILSVGG DVAFGCCAQE QPPITSTNFT INGIKPWQGR
     LPDNIAGTVY MYAGFYCPMK IVYSNAVSWH TLPVSVELPD VTTVSDDFAG HVYSFDDDLT
     AQLYYP
//