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Protein

E3 ubiquitin-protein ligase TRIM68

Gene

TRIM68

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 61RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri93 – 134B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • histone acetyltransferase binding Source: UniProtKB
  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • interferon-gamma-mediated signaling pathway Source: Reactome
  • protein autoubiquitination Source: UniProtKB
  • regulation of androgen receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-877300. Interferon gamma signaling.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM68 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 137
SSA protein SS-56
Short name:
SS-56
Tripartite motif-containing protein 68
Gene namesi
Name:TRIM68
Synonyms:GC109, RNF137, SS56
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:21161. TRIM68.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: LIFEdb
  • cytosol Source: Reactome
  • Golgi apparatus Source: HPA
  • nucleolus Source: HPA
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55128.
OpenTargetsiENSG00000167333.
PharmGKBiPA134939870.

Polymorphism and mutation databases

BioMutaiTRIM68.
DMDMi74748376.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002494371 – 485E3 ubiquitin-protein ligase TRIM68Add BLAST485

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ6AZZ1.
MaxQBiQ6AZZ1.
PaxDbiQ6AZZ1.
PeptideAtlasiQ6AZZ1.
PRIDEiQ6AZZ1.

PTM databases

iPTMnetiQ6AZZ1.
PhosphoSitePlusiQ6AZZ1.

Expressioni

Tissue specificityi

Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.3 Publications

Inductioni

Up-regulated in prostate cancer.

Gene expression databases

BgeeiENSG00000167333.
CleanExiHS_TRIM68.
ExpressionAtlasiQ6AZZ1. baseline and differential.
GenevisibleiQ6AZZ1. HS.

Organism-specific databases

HPAiHPA023455.

Interactioni

Subunit structurei

Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60.1 Publication

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • histone acetyltransferase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120434. 34 interactors.
IntActiQ6AZZ1. 4 interactors.
STRINGi9606.ENSP00000300747.

Structurei

3D structure databases

ProteinModelPortaliQ6AZZ1.
SMRiQ6AZZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 481B30.2/SPRYPROSITE-ProRule annotationAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili207 – 239Sequence analysisAdd BLAST33

Domaini

The RING domain is essential for ubiquitin E3 ligase activity.1 Publication

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 61RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri93 – 134B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118893.
HOVERGENiHBG001357.
InParanoidiQ6AZZ1.
KOiK12033.
OMAiPCYSIGT.
OrthoDBiEOG091G05W2.
PhylomeDBiQ6AZZ1.
TreeFamiTF338674.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AZZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPTALVEAI VEEVACPICM TFLREPMSID CGHSFCHSCL SGLWEIPGES
60 70 80 90 100
QNWGYTCPLC RAPVQPRNLR PNWQLANVVE KVRLLRLHPG MGLKGDLCER
110 120 130 140 150
HGEKLKMFCK EDVLIMCEAC SQSPEHEAHS VVPMEDVAWE YKWELHEALE
160 170 180 190 200
HLKKEQEEAW KLEVGERKRT ATWKIQVETR KQSIVWEFEK YQRLLEKKQP
210 220 230 240 250
PHRQLGAEVA AALASLQREA AETMQKLELN HSELIQQSQV LWRMIAELKE
260 270 280 290 300
RSQRPVRWML QDIQEVLNRS KSWSLQQPEP ISLELKTDCR VLGLREILKT
310 320 330 340 350
YAADVRLDPD TAYSRLIVSE DRKRVHYGDT NQKLPDNPER FYRYNIVLGS
360 370 380 390 400
QCISSGRHYW EVEVGDRSEW GLGVCKQNVD RKEVVYLSPH YGFWVIRLRK
410 420 430 440 450
GNEYRAGTDE YPILSLPVPP RRVGIFVDYE AHDISFYNVT DCGSHIFTFP
460 470 480
RYPFPGRLLP YFSPCYSIGT NNTAPLAICS LDGED
Length:485
Mass (Da):56,259
Last modified:September 13, 2004 - v1
Checksum:iFCB9A6076382E2A5
GO
Isoform 2 (identifier: Q6AZZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MDP → MQK
     4-282: Missing.

Note: No experimental confirmation available.
Show »
Length:206
Mass (Da):23,798
Checksum:i8D38B44D58737F7B
GO

Sequence cautioni

The sequence BAA91569 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14309 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44W → R in BAG51737 (PubMed:14702039).Curated1
Sequence conflicti109 – 114CKEDVL → RGNSLQ in AAF91075 (PubMed:11597395).Curated6
Sequence conflicti175I → V in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti303A → G in AAL31641 (Ref. 2) Curated1
Sequence conflicti304D → V in BAG62716 (PubMed:14702039).Curated1
Sequence conflicti314S → P in BAB14309 (PubMed:14702039).Curated1
Sequence conflicti376K → Y in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti399R → K in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti411Y → V in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti422R → P in AAF91075 (PubMed:11597395).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027415442C → Y.1 PublicationCorresponds to variant rs2231975dbSNPEnsembl.1
Natural variantiVAR_063007457R → H.Corresponds to variant rs2231976dbSNPEnsembl.1
Natural variantiVAR_063008466Y → S.Corresponds to variant rs7109316dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554441 – 3MDP → MQK in isoform 2. 1 Publication3
Alternative sequenceiVSP_0554454 – 282Missing in isoform 2. 1 PublicationAdd BLAST279

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF360739 mRNA. Translation: AAL11501.1.
AF439153 mRNA. Translation: AAL31641.1.
AK001231 mRNA. Translation: BAA91569.1. Different initiation.
AK022923 mRNA. Translation: BAB14309.1. Different initiation.
AK291166 mRNA. Translation: BAF83855.1.
AK301120 mRNA. Translation: BAG62716.1.
AK056523 mRNA. Translation: BAG51737.1.
AC090719 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68835.1.
BC075058 mRNA. Translation: AAH75058.1.
BC109063 mRNA. Translation: AAI09064.1.
AY005802 mRNA. Translation: AAF91075.1.
CCDSiCCDS31356.1. [Q6AZZ1-1]
RefSeqiNP_001291425.1. NM_001304496.1.
NP_060543.5. NM_018073.7. [Q6AZZ1-1]
UniGeneiHs.523438.
Hs.738828.

Genome annotation databases

EnsembliENST00000300747; ENSP00000300747; ENSG00000167333. [Q6AZZ1-1]
GeneIDi55128.
KEGGihsa:55128.
UCSCiuc001lzf.2. human. [Q6AZZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF360739 mRNA. Translation: AAL11501.1.
AF439153 mRNA. Translation: AAL31641.1.
AK001231 mRNA. Translation: BAA91569.1. Different initiation.
AK022923 mRNA. Translation: BAB14309.1. Different initiation.
AK291166 mRNA. Translation: BAF83855.1.
AK301120 mRNA. Translation: BAG62716.1.
AK056523 mRNA. Translation: BAG51737.1.
AC090719 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68835.1.
BC075058 mRNA. Translation: AAH75058.1.
BC109063 mRNA. Translation: AAI09064.1.
AY005802 mRNA. Translation: AAF91075.1.
CCDSiCCDS31356.1. [Q6AZZ1-1]
RefSeqiNP_001291425.1. NM_001304496.1.
NP_060543.5. NM_018073.7. [Q6AZZ1-1]
UniGeneiHs.523438.
Hs.738828.

3D structure databases

ProteinModelPortaliQ6AZZ1.
SMRiQ6AZZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120434. 34 interactors.
IntActiQ6AZZ1. 4 interactors.
STRINGi9606.ENSP00000300747.

PTM databases

iPTMnetiQ6AZZ1.
PhosphoSitePlusiQ6AZZ1.

Polymorphism and mutation databases

BioMutaiTRIM68.
DMDMi74748376.

Proteomic databases

EPDiQ6AZZ1.
MaxQBiQ6AZZ1.
PaxDbiQ6AZZ1.
PeptideAtlasiQ6AZZ1.
PRIDEiQ6AZZ1.

Protocols and materials databases

DNASUi55128.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300747; ENSP00000300747; ENSG00000167333. [Q6AZZ1-1]
GeneIDi55128.
KEGGihsa:55128.
UCSCiuc001lzf.2. human. [Q6AZZ1-1]

Organism-specific databases

CTDi55128.
DisGeNETi55128.
GeneCardsiTRIM68.
HGNCiHGNC:21161. TRIM68.
HPAiHPA023455.
MIMi613184. gene.
neXtProtiNX_Q6AZZ1.
OpenTargetsiENSG00000167333.
PharmGKBiPA134939870.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118893.
HOVERGENiHBG001357.
InParanoidiQ6AZZ1.
KOiK12033.
OMAiPCYSIGT.
OrthoDBiEOG091G05W2.
PhylomeDBiQ6AZZ1.
TreeFamiTF338674.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-877300. Interferon gamma signaling.

Miscellaneous databases

ChiTaRSiTRIM68. human.
GeneWikiiTRIM68.
GenomeRNAii55128.
PROiQ6AZZ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167333.
CleanExiHS_TRIM68.
ExpressionAtlasiQ6AZZ1. baseline and differential.
GenevisibleiQ6AZZ1. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI68_HUMAN
AccessioniPrimary (citable) accession number: Q6AZZ1
Secondary accession number(s): A6NI19
, A8K551, B3KPM5, B4DVK4, Q8WZ70, Q96LE5, Q96PF7, Q9H9C2, Q9NW18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antibodies against TRIM68 are found in patients with systemic lupus erythematosus (SLE) and primary Sjoegren syndrome.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.