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Q6AZZ1 (TRI68_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase TRIM68

EC=6.3.2.-
Alternative name(s):
RING finger protein 137
SSA protein SS-56
Short name=SS-56
Tripartite motif-containing protein 68
Gene names
Name:TRIM68
Synonyms:GC109, RNF137, SS56
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity. Ref.8

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60. Ref.8

Subcellular location

Cytoplasmperinuclear region. Nucleus. Note: Colocalized with AR in nucleus. Ref.1 Ref.8

Tissue specificity

Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells. Ref.1 Ref.7 Ref.8

Induction

Up-regulated in prostate cancer.

Domain

The RING domain is essential for ubiquitin E3 ligase activity. Ref.8

Post-translational modification

Auto-ubiquitinated.

Miscellaneous

Antibodies against TRIM68 are found in patients with systemic lupus erythematosus (SLE) and primary Sjoegren syndrome.

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 1 B box-type zinc finger.

Contains 1 B30.2/SPRY domain.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence BAA91569.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14309.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485E3 ubiquitin-protein ligase TRIM68
PRO_0000249437

Regions

Domain285 – 481197B30.2/SPRY
Zinc finger16 – 6146RING-type
Zinc finger93 – 13442B box-type
Coiled coil207 – 23933 Potential

Natural variations

Natural variant4421C → Y. Ref.1
Corresponds to variant rs2231975 [ dbSNP | Ensembl ].
VAR_027415
Natural variant4571R → H.
Corresponds to variant rs2231976 [ dbSNP | Ensembl ].
VAR_063007
Natural variant4661Y → S.
Corresponds to variant rs7109316 [ dbSNP | Ensembl ].
VAR_063008

Experimental info

Sequence conflict441W → R in BAG51737. Ref.3
Sequence conflict109 – 1146CKEDVL → RGNSLQ in AAF91075. Ref.7
Sequence conflict1751I → V in AAF91075. Ref.7
Sequence conflict3031A → G in AAL31641. Ref.2
Sequence conflict3141S → P in BAB14309. Ref.3
Sequence conflict3761K → Y in AAF91075. Ref.7
Sequence conflict3991R → K in AAF91075. Ref.7
Sequence conflict4111Y → V in AAF91075. Ref.7
Sequence conflict4221R → P in AAF91075. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q6AZZ1 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: FCB9A6076382E2A5

FASTA48556,259
        10         20         30         40         50         60 
MDPTALVEAI VEEVACPICM TFLREPMSID CGHSFCHSCL SGLWEIPGES QNWGYTCPLC 

        70         80         90        100        110        120 
RAPVQPRNLR PNWQLANVVE KVRLLRLHPG MGLKGDLCER HGEKLKMFCK EDVLIMCEAC 

       130        140        150        160        170        180 
SQSPEHEAHS VVPMEDVAWE YKWELHEALE HLKKEQEEAW KLEVGERKRT ATWKIQVETR 

       190        200        210        220        230        240 
KQSIVWEFEK YQRLLEKKQP PHRQLGAEVA AALASLQREA AETMQKLELN HSELIQQSQV 

       250        260        270        280        290        300 
LWRMIAELKE RSQRPVRWML QDIQEVLNRS KSWSLQQPEP ISLELKTDCR VLGLREILKT 

       310        320        330        340        350        360 
YAADVRLDPD TAYSRLIVSE DRKRVHYGDT NQKLPDNPER FYRYNIVLGS QCISSGRHYW 

       370        380        390        400        410        420 
EVEVGDRSEW GLGVCKQNVD RKEVVYLSPH YGFWVIRLRK GNEYRAGTDE YPILSLPVPP 

       430        440        450        460        470        480 
RRVGIFVDYE AHDISFYNVT DCGSHIFTFP RYPFPGRLLP YFSPCYSIGT NNTAPLAICS 


LDGED 

« Hide

References

« Hide 'large scale' references
[1]"SS-56, a novel cellular target of autoantibody responses in Sjogren syndrome and systemic lupus erythematosus."
Billaut-Mulot O., Cocude C., Kolesnitchenko V., Truong M.-J., Chan E.K.L., Hachulla E., de La Tribonniere X., Capron A., Bahr G.M.
J. Clin. Invest. 108:861-869(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, IDENTIFICATION AS AUTOANTIGEN IN AUTOIMMUNE DISEASES, VARIANT TYR-442.
[2]"Cloning and characterization of FLJ10369, a novel Ro/SSA1-related gene on human chromosome 11p15.5."
Rhodes D.A., Allcock R.J.N., Trowsdale J.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[7]"A novel gene on human chromosome 2p24 is differentially expressed between androgen-dependent and androgen-independent prostate cancer cells."
Chang G.T., Steenbeek M., Schippers E., Blok L.J., van Weerden W.M., van Alewijk D.C., Eussen B.H., van Steenbrugge G.J., Brinkmann A.O.
Eur. J. Cancer 37:2129-2134(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-427, TISSUE SPECIFICITY.
[8]"TRIM68 regulates ligand-dependent transcription of androgen receptor in prostate cancer cells."
Miyajima N., Maruyama S., Bohgaki M., Kano S., Shigemura M., Shinohara N., Nonomura K., Hatakeyama S.
Cancer Res. 68:3486-3494(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH KAT5 AND AR, DOMAIN RING, AUTOUBIQUITINATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF360739 mRNA. Translation: AAL11501.1.
AF439153 mRNA. Translation: AAL31641.1.
AK001231 mRNA. Translation: BAA91569.1. Different initiation.
AK022923 mRNA. Translation: BAB14309.1. Different initiation.
AK291166 mRNA. Translation: BAF83855.1.
AK056523 mRNA. Translation: BAG51737.1.
AC090719 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68835.1.
BC075058 mRNA. Translation: AAH75058.1.
BC109063 mRNA. Translation: AAI09064.1.
AY005802 mRNA. Translation: AAF91075.1.
RefSeqNP_060543.5. NM_018073.6.
UniGeneHs.523438.
Hs.738828.

3D structure databases

ProteinModelPortalQ6AZZ1.
SMRQ6AZZ1. Positions 10-133, 305-481.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120434. 5 interactions.
IntActQ6AZZ1. 1 interaction.
STRING9606.ENSP00000300747.

PTM databases

PhosphoSiteQ6AZZ1.

Polymorphism databases

DMDM74748376.

Proteomic databases

PaxDbQ6AZZ1.
PRIDEQ6AZZ1.

Protocols and materials databases

DNASU55128.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300747; ENSP00000300747; ENSG00000167333.
GeneID55128.
KEGGhsa:55128.
UCSCuc001lzf.2. human.

Organism-specific databases

CTD55128.
GeneCardsGC11M004577.
HGNCHGNC:21161. TRIM68.
HPAHPA023455.
MIM613184. gene.
neXtProtNX_Q6AZZ1.
PharmGKBPA134939870.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG252602.
HOVERGENHBG001357.
InParanoidQ6AZZ1.
KOK12033.
OMACYSIGTN.
OrthoDBEOG72ZCDQ.
PhylomeDBQ6AZZ1.
TreeFamTF338674.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ6AZZ1.
BgeeQ6AZZ1.
CleanExHS_TRIM68.
GenevestigatorQ6AZZ1.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR006574. PRY.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTRIM68. human.
GeneWikiTRIM68.
GenomeRNAi55128.
NextBio58790.
PROQ6AZZ1.
SOURCESearch...

Entry information

Entry nameTRI68_HUMAN
AccessionPrimary (citable) accession number: Q6AZZ1
Secondary accession number(s): A6NI19 expand/collapse secondary AC list , A8K551, B3KPM5, Q8WZ70, Q96LE5, Q96PF7, Q9H9C2, Q9NW18
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 13, 2004
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM