Q6AZT2 (SPAST_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	spast
Synonyms:	spg4

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule Nucleus
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Spastin		PRO_0000367138
Regions
	Transmembrane	54 – 74	21	Helical; Potential
	Domain	110 – 185	76	MIT
	Nucleotide binding	366 – 373	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6AZT2 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: FE675BB257AF09DE	FASTA	600	65,853
	10 20 30 40 50 60 MNSPGGRNDK KKPVTPAAET GPGSPTTPPS TETQVVLAPP SPHKRNLHLF SYPLLAVFSL 70 80 90 100 110 120 LRFLAFQLGL LFVWCCELLS RSVMADKGRT VASTAAAQDR PQEPEVVRSY HQQAFQYISL 130 140 150 160 170 180 ALRVDEEEKD QKEQAVQWYK KGIEELEKGI AVPISGKGEQ YDRARRLQAK MSTNLIMAKD 190 200 210 220 230 240 RLQLLAKLQA DIQGPHSQME VCSDNTNLPC RNGLLKPEKG AVPKKKDPPP ITSNSYSRTK 250 260 270 280 290 300 APPKSGSLGN RIPNCTSVPT SARQAGAHTP SNRGATGKNN TRTNKPATPT TAVRKKDMKN 310 320 330 340 350 360 LRNVDSNLAN LILNEIVDSG PSVKFADIAG QDLAKQALQE IVILPSIRPE LFTGLRAPAR 370 380 390 400 410 420 GLLLFGPPGN GKTMLAKAVA AESNATFFNI SAASLTSKYV GEGEKLVRAL FSVARELQPS 430 440 450 460 470 480 IIFIDEVDSL LCERREGEHD ASRRLKTEFL IEFDGVQSGG DDRVLVMGAT NRPQELDDAV 490 500 510 520 530 540 LRRFTKRVYV ALPNEETRLV LLKNLLSKQG NPLSEKELTQ LSRLTEGYSG SDITALAKDA 550 560 570 580 590 600 ALGPIRELKP EQVKNMAASE MRNMKYSDFL GSLKKIKCSV SHSTLESYIR WNQDFGDTTV

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC077358 mRNA. Translation: AAH77358.1.
RefSeq	NP_001086725.1. NM_001093256.1.
UniGene	Xl.32426.
3D structure databases
HSSP	HSSP built from PDB template 1XWI based on UniProtKB O75351.
ProteinModelPortal	Q6AZT2.
SMR	Q6AZT2. Positions 308-592.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	446560.
KEGG	xla:446560.
Organism-specific databases
CTD	6683.
Xenbase	XB-GENE-947846. spast.
Phylogenomic databases
HOVERGEN	HBG108502.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_XENLA

Accession

Primary (citable) accession number: Q6AZT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 13, 2004
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program