Q6AZN6 (PK3C3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase catalytic subunit type 3
Short name=PI3-kinase type 3
Short name=PI3K type 3
Short name=PtdIns-3-kinase type 3
EC=2.7.1.137

Alternative name(s):
Phosphoinositide-3-kinase class 3

Gene names

Name:

pik3c3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	886 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Subcellular location	Midbody By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Biological process	Autophagy Cell cycle Cell division
Ligand	ATP-binding Manganese Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular_component	midbody Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 886	886	Phosphatidylinositol 3-kinase catalytic subunit type 3		PRO_0000088806
Regions
	Domain	35 – 184	150	C2 PI3K-type
	Domain	283 – 519	237	PIK helical
	Domain	630 – 884	255	PI3K/PI4K

Sequences

	Sequence		Length	Mass (Da)
	Q6AZN6 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: FBAFB8847FBB60A9	FASTA	886	101,157
	10 20 30 40 50 60 MGESDRFCYV YSCDLDISVR LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY QETCSDLYVT 70 80 90 100 110 120 CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYADL PRSAQVALTI WDVYGPGKAI 130 140 150 160 170 180 PVGGATVSLF GKYGMFRQGM HDLKVWPNIE ADGSELTKTP GRTNSSASED QMSRLAKLTK 190 200 210 220 230 240 AHRQGHMVKV DWLDRLTFRE IEMINESEKR SSNFMYLMVE FPCVKCDEKE YGIVYYEKDG 250 260 270 280 290 300 DESTPISTSS EIVRVPDPQM SMENLVEIKH HKLARSLRSG PSDHDLKPNA ATRDQLNIIV 310 320 330 340 350 360 SYPPTKQLTS EEQDLVWKFR SYLTSQEKAL TKFLKCVNWD LPQEAKQALE LLGKWKPMDV 370 380 390 400 410 420 EDSLELLSSH FTNPTVRRYA VARLQQADDE DLLMYLLQLV QALKYENFED IKSGLEPTKK 430 440 450 460 470 480 DSQGPMLESM TTSGINPETD SSQILSNPLP AVSSPAPPSK TKDGLDAETL EQDLCTFLIS 490 500 510 520 530 540 RACKNSTLAN YLYWYVIVEC EDQDTQLRDP KTHEMYLNVM RRFSQALLKG DKSVRVMRSL 550 560 570 580 590 600 LATQQTFVDR LVHLMKAVQR ESGNRKKKNE RLQALLGDNE KMNLSEFEPI PLPLEPQVKI 610 620 630 640 650 660 RGIIPEKATL FKSALMPAKL YFKTEDGGKY PVIFKNGDDL RQDQLILQII SLMDKLLRKE 670 680 690 700 710 720 NLDLKLTPYK VLATSTKHGF MQFIQSVPVA EVLATEGSIQ NFFRKYSPSE KGPYGISAEV 730 740 750 760 770 780 MDTYVKSCAG YCVITYILGV GDRHLDNLLL TKTGKLFHID FGYILGRDPK PLPPPMKLNK 790 800 810 820 830 840 EMVEGMGGTQ SEQYQAFRKQ CYTAFLHLRR YSNLILNLFS LMVDANIPDI ALEPDKTVKK 850 860 870 880 VQDKFRLDLS DEEAVHYMQT LIDDSVNALF AAVVEQIHKF AQYWRR

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC077528 mRNA. Translation: AAH77528.1.
RefSeq	NP_001086836.1. NM_001093367.1.
UniGene	Xl.11237.
3D structure databases
ProteinModelPortal	Q6AZN6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	446671.
KEGG	xla:446671.
Organism-specific databases
CTD	5289.
Xenbase	XB-GENE-6085232. pik3c3.
Phylogenomic databases
HOVERGEN	HBG082145.
KO	K00914.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 2 hits.
InterPro	IPR016024. ARM-type_fold. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00239. C2. 1 hit. SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PK3C3_XENLA

Accession

Primary (citable) accession number: Q6AZN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2004
Last modified:	March 6, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program