Q6AZA0 (THIL_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase, mitochondrial
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Name:

acat1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a major role in ketone body metabolism By similarity.
Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Potassium
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion By similarity
	Chain	27 – 420	394	Acetyl-CoA acetyltransferase, mitochondrial		PRO_0000356275
Regions
	Region	251 – 253	3	Coenzyme A binding By similarity
Sites
	Active site	119	1	Acyl-thioester intermediate By similarity
	Active site	378	1	Proton acceptor By similarity
	Active site	406	1	Proton acceptor By similarity
	Metal binding	212	1	Potassium By similarity
	Metal binding	273	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	274	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	276	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	374	1	Potassium; via carbonyl oxygen By similarity
	Binding site	212	1	Coenzyme A By similarity
	Binding site	256	1	Coenzyme A By similarity
	Binding site	277	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AZA0 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 9A9323F4C36674C4	FASTA	420	44,342
	10 20 30 40 50 60 MTSRALYSTR SQLCRHLAHK YLSRSYSTRP SLNEVVIVSA VRTPIGSFKG SLSTLPATKL 70 80 90 100 110 120 GSIAIKGAID KAGIPVEEVK EVYMGNVLQA GEGQAPTRQA LLGAGLPLST PATTINKVCA 130 140 150 160 170 180 SGMKSIMLAS QSLMCGHQDV MVAGGMESMS QVPYIMAREA PPYGGVKMED LIVKDGLTDV 190 200 210 220 230 240 YNKFHMGNCA ENTAKNSSIS REEQDAFAIK SYTLSKAAWE SGILAKEVVP VSIPQRGKPD 250 260 270 280 290 300 VVVKEDEEYR KVDFSKVPKL KAVFLKENGT VTAANASTLN DGAAALVLMT ADAAQRLNVT 310 320 330 340 350 360 PLAKIVAFAD AAVAPIDFPI APAFAVPKVL KAAGIKKEDI AMWEINEAFS VVVLANIKML 370 380 390 400 410 420 DIDPDRVNIN GGAVSLGHPI GMSGARIVGH MVHNLKSGQY GLAGICNGGG GASSIVIQKF

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC078651 mRNA. Translation: AAH78651.1.
IPI	IPI00481049.
RefSeq	NP_001003746.1. NM_001003746.1.
UniGene	Dr.77084.
3D structure databases
HSSP	HSSP built from PDB template 2F2S based on UniProtKB P24752.
ProteinModelPortal	Q6AZA0.
SMR	Q6AZA0. Positions 29-420.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AZA0.
Proteomic databases
PRIDE	Q6AZA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000067448; ENSDARP00000067447; ENSDARG00000045888.
GeneID	445290.
KEGG	dre:445290.
Organism-specific databases
CTD	38.
ZFIN	ZDB-GENE-040808-68. acat1.
Phylogenomic databases
eggNOG	fiNOG14716.
GeneTree	ENSGT00390000009412.
HOGENOM	HBG370930.
HOVERGEN	HBG003112.
InParanoid	Q6AZA0.
OrthoDB	EOG4ZW5B8.
PhylomeDB	Q6AZA0.
Gene expression databases
Bgee	Q6AZA0.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_DANRE

Accession

Primary (citable) accession number: Q6AZA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	September 13, 2004
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program