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Q6AZ83 (G6PC3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glucose-6-phosphatase 3
Short name=G-6-Pase 3
Short name=G6Pase 3
EC=3.1.3.9
Gene names
Name:G6pc3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function By similarity.

Catalytic activity

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Enzyme regulation

Inhibited by vanadate By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in liver and kidney. It is the major glucose-6-phosphatase expressed in the small intestine. Ref.3

Induction

Up-regulated upon fasting. Ref.3

Sequence similarities

Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from direct assay. Source: RGD

glucose 6-phosphate metabolic process

Inferred from direct assay. Source: RGD

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionglucose-6-phosphatase activity

Inferred from direct assay. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Glucose-6-phosphatase 3
PRO_0000334514

Regions

Topological domain1 – 2525Lumenal Potential
Transmembrane26 – 4621Helical; Potential
Topological domain47 – 5610Cytoplasmic Potential
Transmembrane57 – 7721Helical; Potential
Topological domain78 – 11538Lumenal Potential
Transmembrane116 – 13520Helical; Potential
Topological domain136 – 1405Cytoplasmic Potential
Transmembrane141 – 16222Helical; Potential
Topological domain163 – 1675Lumenal Potential
Transmembrane168 – 18619Helical; Potential
Topological domain187 – 19711Cytoplasmic Potential
Transmembrane198 – 21821Helical; Potential
Topological domain219 – 25436Lumenal Potential
Transmembrane255 – 27319Helical; Potential
Topological domain274 – 28310Cytoplasmic Potential
Transmembrane284 – 30421Helical; Potential
Topological domain305 – 3073Lumenal Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 34618Cytoplasmic Potential

Sites

Active site1141Proton donor Potential
Active site1671Nucleophile By similarity
Binding site791Substrate Potential
Binding site1611Substrate Potential

Experimental info

Sequence conflict1061T → I in AAO39165. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6AZ83 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 59531F3030990F2F

FASTA34638,834
        10         20         30         40         50         60 
MESTLSAGIM MAEALQNQLP GLENMWLWVT FLADPKNLFQ FYFPAVYYAS RRLGISLFWI 

        70         80         90        100        110        120 
AFITEWLNLV FKWFLFGDRP FWWVHESGYS AQTPVQIHQF PSSCETGPGS PSGHCMITGA 

       130        140        150        160        170        180 
ALWPVMIAIS SQVASQTRSP WVRVIPGLAY CTFLLAVGLS RVFLLAHFPH QVLAGLLAGV 

       190        200        210        220        230        240 
ILGWLLSPRV PMERELSFYG LTALTLMLGA SLMYWTLFTL GLDLSWSINL ASKWCDRPEW 

       250        260        270        280        290        300 
VLVDSRPFAS LSRDSGSALG LGIALHTPCY AQIRRVHLGN GQKIACFVLA MGLLVFLEWL 

       310        320        330        340 
GHPPQISLFY IFNFLKFTLW PCLVVALVPW MVHTLSAQEA PPIRSS

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a glucose-6-phosphatase catalytic subunit-related sequence expressed in rodent tissues."
Middleditch C., Darakhshan F., Guionie O., Bonnefont J., Burchell A., Clottes E.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[3]"Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats?"
Azzout-Marniche D., Gaudichon C., Blouet C., Bos C., Mathe V., Huneau J.-F., Tome D.
Am. J. Physiol. 292:R1400-R1407(2007) [PubMed: 17158265] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY186240 mRNA. Translation: AAO39165.1.
BC078689 mRNA. Translation: AAH78689.1.
IPIIPI00464827.
RefSeqNP_788266.2. NM_176077.3.
UniGeneRn.66254.

3D structure databases

ProteinModelPortalQ6AZ83.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6AZ83.

Proteomic databases

PRIDEQ6AZ83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028375; ENSRNOP00000028375; ENSRNOG00000020902.
GeneID303565.
KEGGrno:303565.
NMPDRfig|10116.3.peg.6204.
UCSCNM_176077. rat.

Organism-specific databases

CTD92579.
RGD727875. G6pc3.

Phylogenomic databases

eggNOGroNOG06035.
GeneTreeENSGT00510000046465.
HOVERGENHBG003560.
InParanoidQ6AZ83.
OMATFLGDPK.
OrthoDBEOG441QCC.
PhylomeDBQ6AZ83.

Gene expression databases

ArrayExpressQ6AZ83.
GenevestigatorQ6AZ83.

Family and domain databases

InterProIPR016275. Glucose-6-phosphatase.
IPR016118. P_Acid_Pase/Cl_peroxidase_N.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
Gene3DG3DSA:1.20.144.10. P_Acid_Pase/Cl_peroxidase_N. 1 hit.
PfamPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMSSF48317. AcPase_VanPerase. 1 hit.
ProtoNetSearch...

Other

NextBio651620.

Entry information

Entry nameG6PC3_RAT
AccessionPrimary (citable) accession number: Q6AZ83
Secondary accession number(s): Q811R7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 13, 2004
Last modified: September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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