Reviewed, UniProtKB/Swiss-Prot Q6AZ83 (G6PC3_RAT)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphatase 3
      Short name=G-6-Pase 3
      Short name=G6Pase 3
    EC=3.1.3.9

Gene names

Name:

G6pc3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function By similarity.
Catalytic activity	D-glucose 6-phosphate + H2O = D-glucose + phosphate.
Enzyme regulation	Inhibited by vanadate By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in liver and kidney. It is the major glucose-6-phosphatase expressed in the small intestine. Ref.3
Induction	Up-regulated upon fasting. Ref.3
Sequence similarities	Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from direct assay. Source: RGD glucose 6-phosphate metabolic process Inferred from direct assay. Source: RGD phosphate metabolic process Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-6-phosphatase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Glucose-6-phosphatase 3		PRO_0000334514
Regions
	Topological domain	1 – 25	25	Lumenal Potential
	Transmembrane	26 – 46	21	Potential
	Topological domain	47 – 56	10	Cytoplasmic Potential
	Transmembrane	57 – 77	21	Potential
	Topological domain	78 – 115	38	Lumenal Potential
	Transmembrane	116 – 135	20	Potential
	Topological domain	136 – 140	5	Cytoplasmic Potential
	Transmembrane	141 – 162	22	Potential
	Topological domain	163 – 167	5	Lumenal Potential
	Transmembrane	168 – 186	19	Potential
	Topological domain	187 – 197	11	Cytoplasmic Potential
	Transmembrane	198 – 218	21	Potential
	Topological domain	219 – 254	36	Lumenal Potential
	Transmembrane	255 – 273	19	Potential
	Topological domain	274 – 283	10	Cytoplasmic Potential
	Transmembrane	284 – 304	21	Potential
	Topological domain	305 – 307	3	Lumenal Potential
	Transmembrane	308 – 328	21	Potential
	Topological domain	329 – 346	18	Cytoplasmic Potential
Sites
	Active site	114	1	Proton donor Potential
	Active site	167	1	Nucleophile By similarity
	Binding site	79	1	Substrate Potential
	Binding site	161	1	Substrate Potential
Experimental info
	Sequence conflict	106	1	T → I in AAO39165. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6AZ83-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 59531F3030990F2F	FASTA	346	38,834
	10 20 30 40 50 60 MESTLSAGIM MAEALQNQLP GLENMWLWVT FLADPKNLFQ FYFPAVYYAS RRLGISLFWI 70 80 90 100 110 120 AFITEWLNLV FKWFLFGDRP FWWVHESGYS AQTPVQIHQF PSSCETGPGS PSGHCMITGA 130 140 150 160 170 180 ALWPVMIAIS SQVASQTRSP WVRVIPGLAY CTFLLAVGLS RVFLLAHFPH QVLAGLLAGV 190 200 210 220 230 240 ILGWLLSPRV PMERELSFYG LTALTLMLGA SLMYWTLFTL GLDLSWSINL ASKWCDRPEW 250 260 270 280 290 300 VLVDSRPFAS LSRDSGSALG LGIALHTPCY AQIRRVHLGN GQKIACFVLA MGLLVFLEWL 310 320 330 340 GHPPQISLFY IFNFLKFTLW PCLVVALVPW MVHTLSAQEA PPIRSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a glucose-6-phosphatase catalytic subunit-related sequence expressed in rodent tissues." Middleditch C., Darakhshan F., Guionie O., Bonnefont J., Burchell A., Clottes E. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats?" Azzout-Marniche D., Gaudichon C., Blouet C., Bos C., Mathe V., Huneau J.-F., Tome D. Am. J. Physiol. 292:R1400-R1407(2007) [PubMed: 17158265] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY186240 mRNA. Translation: AAO39165.1. BC078689 mRNA. Translation: AAH78689.1.
IPI	IPI00464827.
RefSeq	NP_788266.2.
UniGene	Rn.66254
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000020902. Rattus norvegicus. [Contig view]
GeneID	303565.
KEGG	rno:303565.
NMPDR	fig|10116.3.peg.6204.
Organism-specific databases
RGD	727875. G6pc3.
Phylogenomic databases
HOVERGEN	Q6AZ83.
OMA	Q6AZ83. GIAVLWI.
Gene expression databases
ArrayExpress	Q6AZ83.
Family and domain databases
InterPro	IPR016275. Glucose-6-phosphatase. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
PIRSF	PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	651620.

Entry information

Entry name

G6PC3_RAT

Accession

Primary (citable) accession number: Q6AZ83
Secondary accession number(s): Q811R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 13, 2004
Last modified:	July 7, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)