ID   ATG3_RAT                Reviewed;         314 AA.
AC   Q6AZ50; Q9ESH2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   16-NOV-2011, entry version 54.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG3;
DE            EC=6.3.2.-;
DE   AltName: Full=Autophagy-related protein 3;
DE            Short=APG3-like;
DE   AltName: Full=Preconditioning-inducible gene 1 protein;
GN   Name=Atg3; Synonyms=Apg3l;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Laser M., Li Y., Xu L., Darden A., Wu B.X., Hazard E.S. III,
RA   Crosson C., Ma J.X.;
RT   "Identification and characterization of a novel gene induced by
RT   ischemic preconditioning in the retina.";
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: E2-like enzyme involved in autophagy and mitochondrial
CC       homeostasis. Catalyzes the conjugation of ATG8-like proteins
CC       (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to
CC       phosphatidylethanolamine (PE). PE-conjugation to ATG8-like
CC       proteins is essential for autophagy. Preferred substrate is
CC       MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing
CC       the conjugation of ATG12 to itself, ATG12 conjugation to ATG3
CC       playing a role in mitochondrial homeostasis but not in autophagy.
CC       ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-
CC       E2 complex with ATG3 (By similarity).
CC   -!- SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of
CC       ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- PTM: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a
CC       role in regulation of mitochondrial homeostasis and cell death,
CC       while it is not involved in PE-conjugation to ATG8-like proteins
CC       and autophagy (By similarity).
CC   -!- SIMILARITY: Belongs to the ATG3 family.
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DR   EMBL; AF175224; AAG09182.1; -; mRNA.
DR   EMBL; BC078743; AAH78743.1; -; mRNA.
DR   IPI; IPI00191959; -.
DR   RefSeq; NP_599221.1; NM_134394.1.
DR   UniGene; Rn.3084; -.
DR   ProteinModelPortal; Q6AZ50; -.
DR   STRING; Q6AZ50; -.
DR   PRIDE; Q6AZ50; -.
DR   Ensembl; ENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094.
DR   GeneID; 171415; -.
DR   KEGG; rno:171415; -.
DR   UCSC; NM_134394; rat.
DR   CTD; 64422; -.
DR   RGD; 708464; Atg3.
DR   eggNOG; roNOG06532; -.
DR   GeneTree; ENSGT00390000010308; -.
DR   HOVERGEN; HBG080876; -.
DR   InParanoid; Q6AZ50; -.
DR   OMA; DTYHNAG; -.
DR   OrthoDB; EOG4M398Z; -.
DR   PhylomeDB; Q6AZ50; -.
DR   NextBio; 622307; -.
DR   ArrayExpress; Q6AZ50; -.
DR   Genevestigator; Q6AZ50; -.
DR   GermOnline; ENSRNOG00000002094; Rattus norvegicus.
DR   GO; GO:0005829; C:cytosol; IDA:RGD.
DR   GO; GO:0019777; F:Atg12 ligase activity; ISS:UniProtKB.
DR   GO; GO:0019776; F:Atg8 ligase activity; ISS:UniProtKB.
DR   GO; GO:0000045; P:autophagic vacuole assembly; ISS:UniProtKB.
DR   GO; GO:0043653; P:mitochondrial fragmentation involved in apoptosis; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Autophagy-rel_prot_3.
DR   InterPro; IPR019461; Autophagy-rel_prot_3_C.
DR   InterPro; IPR007134; Autophagy-rel_prot_3_N.
DR   KO; K08343; -.
DR   Pfam; PF03987; Autophagy_act_C; 1.
DR   Pfam; PF10381; Autophagy_Cterm; 1.
DR   Pfam; PF03986; Autophagy_N; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Autophagy; Complete proteome; Cytoplasm; Isopeptide bond;
KW   Ligase; Phosphoprotein; Protein transport; Reference proteome;
KW   Transport; Ubl conjugation; Ubl conjugation pathway.
FT   CHAIN         1    314       Ubiquitin-like-conjugating enzyme ATG3.
FT                                /FTId=PRO_0000213571.
FT   ACT_SITE    264    264       Glycyl thioester intermediate (By
FT                                similarity).
FT   MOD_RES       1      1       N-acetylmethionine (By similarity).
FT   MOD_RES      18     18       Phosphotyrosine (By similarity).
FT   CROSSLNK    243    243       Glycyl lysine isopeptide (Lys-Gly)
FT                                (interchain with G-Cter in ATG12) (By
FT                                similarity).
FT   CONFLICT    257    257       P -> L (in Ref. 1; AAG09182).
SQ   SEQUENCE   314 AA;  35822 MW;  4C78AB0F628C9BDF CRC64;
     MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE
     LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT
     EAVKEITLES KDSIKLQDCS VLCDEEEEEE EGEAADMEEY EESGLLETDE ATLDTRRIVE
     ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH
     VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV
     IPTIEYDYTR HFTM
//