Reviewed, UniProtKB/Swiss-Prot Q6AZ50 (ATG3_RAT)

Names and origin

Protein names

Recommended name:
    Autophagy-related protein 3
Alternative name(s):
    APG3-like
    Preconditioning-inducible gene 1 protein

Gene names

Name:	Atg3
Synonyms:	Apg3l

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy By similarity.
Subunit structure	Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ATG3 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from expression pattern. Source: RGD modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Autophagy-related protein 3		PRO_0000213571
Sites
	Active site	264	1	Glycyl thioester intermediate Potential
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	18	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	257	1	P → L in AAG09182. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6AZ50-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 4C78AB0F628C9BDF	FASTA	314	35,822
	10 20 30 40 50 60 MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 70 80 90 100 110 120 LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 130 140 150 160 170 180 EAVKEITLES KDSIKLQDCS VLCDEEEEEE EGEAADMEEY EESGLLETDE ATLDTRRIVE 190 200 210 220 230 240 ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH 250 260 270 280 290 300 VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV 310 IPTIEYDYTR HFTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel gene induced by ischemic preconditioning in the retina." Laser M., Li Y., Xu L., Darden A., Wu B.X., Hazard E.S. III, Crosson C., Ma J.X. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175224 mRNA. Translation: AAG09182.1. BC078743 mRNA. Translation: AAH78743.1.
IPI	IPI00191959.
RefSeq	NP_599221.1.
UniGene	Rn.3084
3D structure databases
SMR	Q6AZ50. Positions 27-309.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AZ50.
Genome annotation databases
Ensembl	ENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094; Rattus norvegicus. [Genome view]
GeneID	171415.
KEGG	rno:171415.
UCSC	NM_134394. rat.
Organism-specific databases
CTD	171415.
RGD	708464. Atg3.
Phylogenomic databases
eggNOG	roNOG06532.
HOVERGEN	Q6AZ50.
InParanoid	Q6AZ50.
OMA	YHNTGIA.
OrthoDB	EOG9WWV4X.
PhylomeDB	Q6AZ50.
Gene expression databases
ArrayExpress	Q6AZ50.
Genevestigator	Q6AZ50.
GermOnline	ENSRNOG00000002094. Rattus norvegicus.
Family and domain databases
InterPro	IPR007135. Autophagy-rel_prot_3. IPR019461. Autophagy-rel_prot_3_C. IPR007134. Autophagy-rel_prot_3_N. [Graphical view]
Pfam	PF03987. Autophagy_act_C. 1 hit. PF10381. Autophagy_Cterm. 1 hit. PF03986. Autophagy_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622307.

Entry information

Entry name

ATG3_RAT

Accession

Primary (citable) accession number: Q6AZ50
Secondary accession number(s): Q9ESH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)