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Protein

Ubiquitin-like-conjugating enzyme ATG3

Gene

Atg3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641Glycyl thioester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-conjugating enzyme ATG3 (EC:6.3.2.-)
Alternative name(s):
Autophagy-related protein 3
Short name:
APG3-like
Preconditioning-inducible gene 1 protein
Gene namesi
Name:Atg3
Synonyms:Apg3l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi708464. Atg3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic ubiquitin ligase complex Source: Ensembl
  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Ubiquitin-like-conjugating enzyme ATG3PRO_0000213571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Cross-linki243 – 243Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)By similarity

Post-translational modificationi

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy (By similarity).By similarity
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ6AZ50.
PRIDEiQ6AZ50.

PTM databases

PhosphoSiteiQ6AZ50.

Expressioni

Gene expression databases

GenevisibleiQ6AZ50. RN.

Interactioni

Subunit structurei

Interacts with ATG7 and ATG12. The complex composed of ATG3 and ATG7 plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.By similarity

Protein-protein interaction databases

BioGridi251241. 1 interaction.
STRINGi10116.ENSRNOP00000002906.

Structurei

3D structure databases

ProteinModelPortaliQ6AZ50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG3 family.Curated

Phylogenomic databases

eggNOGiKOG2981. Eukaryota.
ENOG410Y3BC. LUCA.
GeneTreeiENSGT00390000010308.
HOGENOMiHOG000234613.
HOVERGENiHBG080876.
InParanoidiQ6AZ50.
KOiK08343.
OMAiDQMKEDV.
OrthoDBiEOG7VMP5R.
PhylomeDBiQ6AZ50.
TreeFamiTF105903.

Family and domain databases

InterProiIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_C. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AZ50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC
60 70 80 90 100
PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE
110 120 130 140 150
DDGDGGWVDT YHNTGITGIT EAVKEITLES KDSIKLQDCS VLCDEEEEEE
160 170 180 190 200
EGEAADMEEY EESGLLETDE ATLDTRRIVE ACKAKADAGG EDAILQTRTY
210 220 230 240 250
DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH VKKTVTIENH
260 270 280 290 300
PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV
310
IPTIEYDYTR HFTM
Length:314
Mass (Da):35,822
Last modified:September 13, 2004 - v1
Checksum:i4C78AB0F628C9BDF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti257 – 2571P → L in AAG09182 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175224 mRNA. Translation: AAG09182.1.
BC078743 mRNA. Translation: AAH78743.1.
RefSeqiNP_599221.1. NM_134394.2.
UniGeneiRn.3084.

Genome annotation databases

EnsembliENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094.
GeneIDi171415.
KEGGirno:171415.
UCSCiRGD:708464. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175224 mRNA. Translation: AAG09182.1.
BC078743 mRNA. Translation: AAH78743.1.
RefSeqiNP_599221.1. NM_134394.2.
UniGeneiRn.3084.

3D structure databases

ProteinModelPortaliQ6AZ50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251241. 1 interaction.
STRINGi10116.ENSRNOP00000002906.

PTM databases

PhosphoSiteiQ6AZ50.

Proteomic databases

PaxDbiQ6AZ50.
PRIDEiQ6AZ50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094.
GeneIDi171415.
KEGGirno:171415.
UCSCiRGD:708464. rat.

Organism-specific databases

CTDi64422.
RGDi708464. Atg3.

Phylogenomic databases

eggNOGiKOG2981. Eukaryota.
ENOG410Y3BC. LUCA.
GeneTreeiENSGT00390000010308.
HOGENOMiHOG000234613.
HOVERGENiHBG080876.
InParanoidiQ6AZ50.
KOiK08343.
OMAiDQMKEDV.
OrthoDBiEOG7VMP5R.
PhylomeDBiQ6AZ50.
TreeFamiTF105903.

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.

Miscellaneous databases

NextBioi622307.
PROiQ6AZ50.

Gene expression databases

GenevisibleiQ6AZ50. RN.

Family and domain databases

InterProiIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_C. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel gene induced by ischemic preconditioning in the retina."
    Laser M., Li Y., Xu L., Darden A., Wu B.X., Hazard E.S. III, Crosson C., Ma J.X.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiATG3_RAT
AccessioniPrimary (citable) accession number: Q6AZ50
Secondary accession number(s): Q9ESH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.