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Protein

Ubiquitin-like-conjugating enzyme ATG3

Gene

Atg3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264Glycyl thioester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like-conjugating enzyme ATG3 (EC:2.3.2.-)
Alternative name(s):
Autophagy-related protein 3
Short name:
APG3-like
Preconditioning-inducible gene 1 protein
Gene namesi
Name:Atg3
Synonyms:Apg3l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi708464 Atg3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135711 – 314Ubiquitin-like-conjugating enzyme ATG3Add BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki243Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)By similarity

Post-translational modificationi

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy (By similarity).By similarity
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ6AZ50
PRIDEiQ6AZ50

PTM databases

PhosphoSitePlusiQ6AZ50

Expressioni

Gene expression databases

BgeeiENSRNOG00000002094
GenevisibleiQ6AZ50 RN

Interactioni

Subunit structurei

Interacts with ATG7 and ATG12. The complex composed of ATG3 and ATG7 plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi251241, 1 interactor
STRINGi10116.ENSRNOP00000002906

Structurei

3D structure databases

ProteinModelPortaliQ6AZ50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG3 family.Curated

Phylogenomic databases

eggNOGiKOG2981 Eukaryota
ENOG410Y3BC LUCA
GeneTreeiENSGT00390000010308
HOGENOMiHOG000234613
HOVERGENiHBG080876
InParanoidiQ6AZ50
KOiK08343
OMAiYEDVSQD
OrthoDBiEOG091G0H8F
PhylomeDBiQ6AZ50
TreeFamiTF105903

Family and domain databases

InterProiView protein in InterPro
IPR007135 Autophagy-rel_prot_3
IPR019461 Autophagy-rel_prot_3_C
IPR007134 Autophagy-rel_prot_3_N
PfamiView protein in Pfam
PF03987 Autophagy_act_C, 1 hit
PF10381 Autophagy_C, 1 hit
PF03986 Autophagy_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AZ50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC
60 70 80 90 100
PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE
110 120 130 140 150
DDGDGGWVDT YHNTGITGIT EAVKEITLES KDSIKLQDCS VLCDEEEEEE
160 170 180 190 200
EGEAADMEEY EESGLLETDE ATLDTRRIVE ACKAKADAGG EDAILQTRTY
210 220 230 240 250
DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH VKKTVTIENH
260 270 280 290 300
PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV
310
IPTIEYDYTR HFTM
Length:314
Mass (Da):35,822
Last modified:September 13, 2004 - v1
Checksum:i4C78AB0F628C9BDF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257P → L in AAG09182 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175224 mRNA Translation: AAG09182.1
BC078743 mRNA Translation: AAH78743.1
RefSeqiNP_599221.1, NM_134394.2
UniGeneiRn.3084

Genome annotation databases

EnsembliENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094
GeneIDi171415
KEGGirno:171415
UCSCiRGD:708464 rat

Similar proteinsi

Entry informationi

Entry nameiATG3_RAT
AccessioniPrimary (citable) accession number: Q6AZ50
Secondary accession number(s): Q9ESH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: September 13, 2004
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health