Q6AZ50 (ATG3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like-conjugating enzyme ATG3
EC=6.3.2.-

Alternative name(s):
Autophagy-related protein 3
Short name=APG3-like
Preconditioning-inducible gene 1 protein

Gene names

Name:	Atg3
Synonyms:	Apg3l

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 By similarity.
Subunit structure	Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy By similarity.
Sequence similarities	Belongs to the ATG3 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Ligase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagic vacuole assembly Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial fragmentation involved in apoptosis Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: RGD
Molecular function	Atg12 ligase activity Inferred from sequence or structural similarity. Source: UniProtKB Atg8 ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Ubiquitin-like-conjugating enzyme ATG3		PRO_0000213571
Sites
	Active site	264	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	18	1	Phosphotyrosine By similarity
	Cross-link	243		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12) By similarity
Experimental info
	Sequence conflict	257	1	P → L in AAG09182. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6AZ50 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 4C78AB0F628C9BDF	FASTA	314	35,822
	10 20 30 40 50 60 MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 70 80 90 100 110 120 LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 130 140 150 160 170 180 EAVKEITLES KDSIKLQDCS VLCDEEEEEE EGEAADMEEY EESGLLETDE ATLDTRRIVE 190 200 210 220 230 240 ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH 250 260 270 280 290 300 VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV 310 IPTIEYDYTR HFTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel gene induced by ischemic preconditioning in the retina." Laser M., Li Y., Xu L., Darden A., Wu B.X., Hazard E.S. III, Crosson C., Ma J.X. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175224 mRNA. Translation: AAG09182.1. BC078743 mRNA. Translation: AAH78743.1.
IPI	IPI00191959.
RefSeq	NP_599221.1. NM_134394.1.
UniGene	Rn.3084.
3D structure databases
ProteinModelPortal	Q6AZ50.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AZ50.
Proteomic databases
PRIDE	Q6AZ50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000002906; ENSRNOP00000002906; ENSRNOG00000002094.
GeneID	171415.
KEGG	rno:171415.
UCSC	NM_134394. rat.
Organism-specific databases
CTD	64422.
RGD	708464. Atg3.
Phylogenomic databases
eggNOG	roNOG06532.
GeneTree	ENSGT00390000010308.
HOVERGEN	HBG080876.
InParanoid	Q6AZ50.
OMA	DTYHNAG.
OrthoDB	EOG4M398Z.
PhylomeDB	Q6AZ50.
Gene expression databases
ArrayExpress	Q6AZ50.
Genevestigator	Q6AZ50.
GermOnline	ENSRNOG00000002094. Rattus norvegicus.
Family and domain databases
InterPro	IPR007135. Autophagy-rel_prot_3. IPR019461. Autophagy-rel_prot_3_C. IPR007134. Autophagy-rel_prot_3_N. [Graphical view]
KO	K08343.
Pfam	PF03987. Autophagy_act_C. 1 hit. PF10381. Autophagy_Cterm. 1 hit. PF03986. Autophagy_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622307.

Entry information

Entry name

ATG3_RAT

Accession

Primary (citable) accession number: Q6AZ50
Secondary accession number(s): Q9ESH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	September 13, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program