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Protein

Outer dense fiber protein 2

Gene

Odf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • spermatid development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer dense fiber protein 2
Alternative name(s):
84 kDa outer dense fiber protein
Cenexin
Outer dense fiber of sperm tails protein 2
Gene namesi
Name:Odf2
Synonyms:Odf84
ORF Names:KKT4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi3229. Odf2.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • ciliary transition fiber Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • nucleus Source: Ensembl
  • outer dense fiber Source: RGD
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002994601 – 825Outer dense fiber protein 2Add BLAST825

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei87PhosphothreonineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei104PhosphoserineCombined sources1
Modified residuei105PhosphothreonineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei124PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei226PhosphothreonineBy similarity1
Modified residuei256PhosphoserineCombined sources1
Modified residuei627PhosphoserineCombined sources1
Isoform 3 (identifier: Q6AYX5-3)
Modified residuei22PhosphoserineCombined sourcesCurated1
Modified residuei37PhosphoserineCombined sourcesCurated1

Post-translational modificationi

Tyrosine phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AYX5.
PRIDEiQ6AYX5.

PTM databases

iPTMnetiQ6AYX5.
PhosphoSitePlusiQ6AYX5.

Expressioni

Tissue specificityi

Testis-specific (PubMed:9045620). Expressed in the proximal compartment of the elongated spermatid tail; later expression progresses to the distal spermatid tail compartment located in the lumen of the seminiferous epithelium (PubMed:9698445). In spermatids (stages II-III) expression of the tails peaks and remains strong during the remaining steps of spermiogenesis (at protein level) (PubMed:9698445). Expression correlates with the onset of spermatogenesis and is first detected at 30 days. Higher expression is seen in testis of 40-day-old and adults that are older than 50 days (PubMed:9092585). No expression is seen in 10- and 20-day-old testes (PubMed:9092585).4 Publications

Gene expression databases

BgeeiENSRNOG00000014584.
ExpressionAtlasiQ6AYX5. differential.
GenevisibleiQ6AYX5. RN.

Interactioni

Subunit structurei

Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts through its C-terminus with PLK1. Interacts with ODF1 (PubMed:9045620). Interacts with MARK4; the interaction is required for localization of ODF2 to centrioles (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041336.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili139 – 212Sequence analysisAdd BLAST74
Coiled coili240 – 418Sequence analysisAdd BLAST179
Coiled coili456 – 793Sequence analysisAdd BLAST338

Sequence similaritiesi

Belongs to the ODF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IE8V. Eukaryota.
ENOG4111XWH. LUCA.
GeneTreeiENSGT00530000063497.
HOGENOMiHOG000246967.
HOVERGENiHBG052808.
InParanoidiQ6AYX5.
KOiK16479.
OMAiKEHAASK.
OrthoDBiEOG091G13KV.
PhylomeDBiQ6AYX5.

Family and domain databases

InterProiIPR026099. Odf2-rel.
[Graphical view]
PANTHERiPTHR23162. PTHR23162. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AYX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKDRSSTPPL HVHVDENTPV HVHIKKLPKP SAASSQKSHK RGMKGDTVNV
60 70 80 90 100
RRSVRVKTKV PWMPPGKSSA RHVGCKWENP PHCLEITPPS SEKLVSVMRL
110 120 130 140 150
SDLSTEDDDS GHCKMNRYDK KIDSLMNAVG CLKSEVKMQK GERQMAKRFL
160 170 180 190 200
EERKEELEEV AHELAETEHE NTVLRHNIER IKEEKDFTML QKKHLQQEKE
210 220 230 240 250
CLMSKLVEAE MDGAAAAKQV MALKDTIGKL KTEKQMTCTD INTLTRQKEL
260 270 280 290 300
LLQKLSTFEE TNRTLRDLLR EQHCKEDSER LMEQQGALLK RLAEADSEKA
310 320 330 340 350
RLLLLLQDKD KEVEELLQEI QCEKAQAKTA SELSKSMESM RGHLQAQLRC
360 370 380 390 400
KEAENSRLCM QIKNLERSGN QHKAEVEAIM EQLKELKQKG DRDKETLKKA
410 420 430 440 450
IRAQKERAEK SEEYAEQLHV QLADKDLYVA EALSTLESWR SRYNQVVKDK
460 470 480 490 500
GDLELEIIVL NDRVTDLVNQ QQSLEEKMRE DRDSLVERLH RQTAEYSAFK
510 520 530 540 550
LENERLKASF APMEDKLNQA HLEVQQLKAS VKNYEGMIDN YKSQVMKTRL
560 570 580 590 600
EADEVAAQLE RCDKENKMLK DEMNKEIEAA RRQFQSQLAD LQQLPDILKI
610 620 630 640 650
TEAKLAECQD QLQGYERKNI DLTAIISDLR SRIEHQGDKL ELAREKHQAS
660 670 680 690 700
QKENKQLSQK VDELERKLEA TSTQNVEFLQ VIAKREEAIH QAQLRLEEKT
710 720 730 740 750
RECGSLARQL ESAIEDARRQ VEQTKEQALS KERAAQSKIL DLETQLSRTK
760 770 780 790 800
TELGQLRRTR DDVDRRYQSR LQDLKDRLEQ SESTNRSMQN YVQFLKSSYA
810 820
NVFGDGPYTS SYLTSSPIRS RSPPA
Length:825
Mass (Da):95,440
Last modified:September 11, 2007 - v2
Checksum:iFF1490FF9F96CEC7
GO
Isoform 2 (identifier: Q6AYX5-2) [UniParc]FASTAAdd to basket
Also known as: Cenexin 2

The sequence of this isoform differs from the canonical sequence as follows:
     763-769: VDRRYQS → ENQKCWK
     770-825: Missing.

Show »
Length:769
Mass (Da):89,095
Checksum:iEF6EFE3003F8365D
GO
Isoform 3 (identifier: Q6AYX5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MKDRSSTPPL...LPKPSAASSQ → MSASSSGGSP...PCGAPSVTVT
     60-78: Missing.
     633-652: IEHQGDKLELAREKHQASQK → VRDWQKGSHELARAGARLPR
     653-825: Missing.

Show »
Length:638
Mass (Da):73,359
Checksum:iC3DAA2F22ACA0A34
GO
Isoform 4 (identifier: Q6AYX5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     60-78: Missing.
     633-652: IEHQGDKLELAREKHQASQK → VRDWQKGSHELARAGARLPR
     653-825: Missing.

Show »
Length:591
Mass (Da):68,600
Checksum:i32B0FBFF3EC3DA40
GO
Isoform 5 (identifier: Q6AYX5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     60-78: Missing.
     276-276: E → ELCLKVPECARQHRPGRERQEDCQ
     630-652: RSRIEHQGDKLELAREKHQASQK → HSRVRDWQKGSHELARAGARLPR
     653-825: Missing.

Show »
Length:614
Mass (Da):71,315
Checksum:i291E30388CB3CEEE
GO

Sequence cautioni

The sequence CAA64567 differs from that shown. Reason: Frameshift at position 92.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46D → Y in AAC53134 (PubMed:9092585).Curated1
Sequence conflicti107 – 108DD → EE in CAA64567 (PubMed:9369191).Curated2
Sequence conflicti120K → E in AAC53134 (PubMed:9092585).Curated1
Sequence conflicti483D → H in AAC53134 (PubMed:9092585).Curated1
Sequence conflicti511A → P in AAC08408 (PubMed:9698445).Curated1
Sequence conflicti543S → T in CAA64567 (PubMed:9369191).Curated1
Isoform 3 (identifier: Q6AYX5-3)
Sequence conflicti25Q → E in AAC08408 (PubMed:9698445).Combined sourcesCurated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276771 – 42Missing in isoform 4 and isoform 5. 2 PublicationsAdd BLAST42
Alternative sequenceiVSP_0276781 – 36MKDRS…AASSQ → MSASSSGGSPRFPSCGKNGV TSLTQKKVLRTPCGAPSVTV T in isoform 3. 2 PublicationsAdd BLAST36
Alternative sequenceiVSP_02767960 – 78Missing in isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST19
Alternative sequenceiVSP_027680276E → ELCLKVPECARQHRPGRERQ EDCQ in isoform 5. 1 Publication1
Alternative sequenceiVSP_027681630 – 652RSRIE…QASQK → HSRVRDWQKGSHELARAGAR LPR in isoform 5. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_027682633 – 652IEHQG…QASQK → VRDWQKGSHELARAGARLPR in isoform 3 and isoform 4. 3 PublicationsAdd BLAST20
Alternative sequenceiVSP_027683653 – 825Missing in isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST173
Alternative sequenceiVSP_027684763 – 769VDRRYQS → ENQKCWK in isoform 2. 1 Publication7
Alternative sequenceiVSP_027685770 – 825Missing in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95272 mRNA. Translation: CAA64567.1. Frameshift.
U62821 mRNA. Translation: AAC53134.1.
AF053971 mRNA. Translation: AAC08408.1.
AF162756 mRNA. Translation: AAF80473.2.
AABR03024548 Genomic DNA. No translation available.
BC078857 mRNA. Translation: AAH78857.1.
RefSeqiNP_001138477.1. NM_001145005.1.
NP_058909.2. NM_017213.2. [Q6AYX5-3]
XP_017447024.1. XM_017591535.1. [Q6AYX5-1]
UniGeneiRn.144353.
Rn.91211.

Genome annotation databases

EnsembliENSRNOT00000040457; ENSRNOP00000049041; ENSRNOG00000014584. [Q6AYX5-3]
ENSRNOT00000041921; ENSRNOP00000041336; ENSRNOG00000014584. [Q6AYX5-1]
GeneIDi29479.
KEGGirno:29479.
UCSCiRGD:3229. rat. [Q6AYX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95272 mRNA. Translation: CAA64567.1. Frameshift.
U62821 mRNA. Translation: AAC53134.1.
AF053971 mRNA. Translation: AAC08408.1.
AF162756 mRNA. Translation: AAF80473.2.
AABR03024548 Genomic DNA. No translation available.
BC078857 mRNA. Translation: AAH78857.1.
RefSeqiNP_001138477.1. NM_001145005.1.
NP_058909.2. NM_017213.2. [Q6AYX5-3]
XP_017447024.1. XM_017591535.1. [Q6AYX5-1]
UniGeneiRn.144353.
Rn.91211.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041336.

PTM databases

iPTMnetiQ6AYX5.
PhosphoSitePlusiQ6AYX5.

Proteomic databases

PaxDbiQ6AYX5.
PRIDEiQ6AYX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040457; ENSRNOP00000049041; ENSRNOG00000014584. [Q6AYX5-3]
ENSRNOT00000041921; ENSRNOP00000041336; ENSRNOG00000014584. [Q6AYX5-1]
GeneIDi29479.
KEGGirno:29479.
UCSCiRGD:3229. rat. [Q6AYX5-1]

Organism-specific databases

CTDi4957.
RGDi3229. Odf2.

Phylogenomic databases

eggNOGiENOG410IE8V. Eukaryota.
ENOG4111XWH. LUCA.
GeneTreeiENSGT00530000063497.
HOGENOMiHOG000246967.
HOVERGENiHBG052808.
InParanoidiQ6AYX5.
KOiK16479.
OMAiKEHAASK.
OrthoDBiEOG091G13KV.
PhylomeDBiQ6AYX5.

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ6AYX5.

Gene expression databases

BgeeiENSRNOG00000014584.
ExpressionAtlasiQ6AYX5. differential.
GenevisibleiQ6AYX5. RN.

Family and domain databases

InterProiIPR026099. Odf2-rel.
[Graphical view]
PANTHERiPTHR23162. PTHR23162. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODFP2_RAT
AccessioniPrimary (citable) accession number: Q6AYX5
Secondary accession number(s): O70408
, P97575, Q62693, Q9JIZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 30, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.