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Protein

DnaJ homolog subfamily B member 6

Gene

Dnajb6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

ReactomeiR-RNO-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 6
Alternative name(s):
Heat shock protein J2
Short name:
HSJ-2
Hsp40 homolog
MRJ
MSJ-1
Gene namesi
Name:Dnajb6Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1308207. Dnajb6.

Subcellular locationi

  • Cytoplasmperinuclear region By similarity
  • Nucleus By similarity
  • CytoplasmmyofibrilsarcomereZ line By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002923402 – 357DnaJ homolog subfamily B member 6Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ6AYU3.
PRIDEiQ6AYU3.

PTM databases

iPTMnetiQ6AYU3.
PhosphoSitePlusiQ6AYU3.

Expressioni

Tissue specificityi

Highest levels of expression found in brain and retina, and lower levels in heart, kidney, liver and placenta.1 Publication

Developmental stagei

Low levels detected in testes at day 7 postnatally, with 10-fold increased levels detected by day 28, remaining into adulthood.1 Publication

Gene expression databases

BgeeiENSRNOG00000010353.
GenevisibleiQ6AYU3. RN.

Interactioni

Subunit structurei

Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with HSP70, KRT18 and PTTG. Interacts with ALKBH1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041883.

Structurei

3D structure databases

ProteinModelPortaliQ6AYU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 69JPROSITE-ProRule annotationAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 147Interaction with HSP70By similarityAdd BLAST146
Regioni120 – 243Interaction with KRT18By similarityAdd BLAST124

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 81Poly-GlySequence analysis7
Compositional biasi84 – 165Phe-richSequence analysisAdd BLAST82
Compositional biasi249 – 299Pro-richSequence analysisAdd BLAST51
Compositional biasi348 – 351Poly-LysSequence analysis4

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ6AYU3.
KOiK09512.
OMAiFFMPTSG.
OrthoDBiEOG091G152F.
PhylomeDBiQ6AYU3.
TreeFamiTF105142.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYYEVLGV QRHASPEDIK KAYRKQALKW HPDKNPENKE EAERKFKQVA
60 70 80 90 100
EAYEVLSDAK KRDIYDKYGK EGLNGGGGGG GSHFDSPFEF GFTFRNPDDV
110 120 130 140 150
FREFFGGRDP FSFDFFEDPF DDFFGNRRGP RGSRSRGAGS FFSAFSGFPS
160 170 180 190 200
FGSGFPAFDT GFTPFGSLGH GGLTSFSSAS FGGSGMGNFK SISTSTKIVN
210 220 230 240 250
GKKITTKRIV ENGQERVEVE EDGQLKSLTI NGVADENALA EECRRRGQPT
260 270 280 290 300
PALAPGPAPA PARVPSQARP PTPAPTPAPA QTPAPSVSTR PQKPPRPAPT
310 320 330 340 350
AKLVSKSNWE DEEQDRQRVP GNCDAPMTSA GLKEGGKRKK QKQKEDSKKK

KSTKGNH
Length:357
Mass (Da):38,922
Last modified:September 13, 2004 - v1
Checksum:i55899E4DE4DF9E76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078908 mRNA. Translation: AAH78908.1.
RefSeqiNP_001013227.1. NM_001013209.1.
UniGeneiRn.129050.

Genome annotation databases

EnsembliENSRNOT00000035996; ENSRNOP00000041883; ENSRNOG00000010353.
GeneIDi362293.
KEGGirno:362293.
UCSCiRGD:1308207. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078908 mRNA. Translation: AAH78908.1.
RefSeqiNP_001013227.1. NM_001013209.1.
UniGeneiRn.129050.

3D structure databases

ProteinModelPortaliQ6AYU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041883.

PTM databases

iPTMnetiQ6AYU3.
PhosphoSitePlusiQ6AYU3.

Proteomic databases

PaxDbiQ6AYU3.
PRIDEiQ6AYU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035996; ENSRNOP00000041883; ENSRNOG00000010353.
GeneIDi362293.
KEGGirno:362293.
UCSCiRGD:1308207. rat.

Organism-specific databases

CTDi10049.
RGDi1308207. Dnajb6.

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ6AYU3.
KOiK09512.
OMAiFFMPTSG.
OrthoDBiEOG091G152F.
PhylomeDBiQ6AYU3.
TreeFamiTF105142.

Enzyme and pathway databases

ReactomeiR-RNO-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

PROiQ6AYU3.

Gene expression databases

BgeeiENSRNOG00000010353.
GenevisibleiQ6AYU3. RN.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJB6_RAT
AccessioniPrimary (citable) accession number: Q6AYU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.