Reviewed, UniProtKB/Swiss-Prot Q6AYT9 (ACSM5_RAT)

Names and origin

Protein names

Recommended name:
    Acyl-coenzyme A synthetase ACSM5, mitochondrial
    EC=6.2.1.2

Gene names

Name:	Acsm5
Synonyms:	Macs3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) By similarity.
Catalytic activity	ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 578	556	Acyl-coenzyme A synthetase ACSM5, mitochondrial		PRO_0000306103
Regions
	Nucleotide binding	229 – 237	9	ATP By similarity
	Nucleotide binding	367 – 372	6	ATP By similarity
	Compositional bias	26 – 32	7	Poly-Pro
Sites
	Binding site	454	1	ATP By similarity
	Binding site	469	1	ATP By similarity
	Binding site	565	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AYT9-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 0F2C8720E03E44CA	FASTA	578	64,623
	10 20 30 40 50 60 MRLWLRGLVY QARRSSWGVF RIHTQPPPPP IPEVVATWEA ISLGKRPVPE YFNFAHDVLD 70 80 90 100 110 120 VWSQLEKTGH RPPNPAFWWV NGSGTEVKWT FEELGKQSRK AANILEGACG LKPGDRLMLV 130 140 150 160 170 180 LPRLPEWWLT IVACMRTGVV MIPGISQLTQ KDLKYRLQAA RVKSIITSDA LAPHVDAISA 190 200 210 220 230 240 DCPSLQSRLL VSDTSRPGWI NFRELLRVAS PEHNCLRTRS GDSMAIYFTS GTTGTPKMVE 250 260 270 280 290 300 HSQCSYGLGF VASGRRLMAL TESDIFWNTT DTGWVKAAWT LFSAWANGAC VFVHELPQVD 310 320 330 340 350 360 AQTILNTLCR FPITTICCVP TLFRLLVQED LTRYKFQCLR HCLAGGEALN SDVRDKWKNQ 370 380 390 400 410 420 TGLEIHEGYG QSETVLICGN FRGSTIKSGS MGKASPPYDV QIVDEEGNVL PPGKEGNIAI 430 440 450 460 470 480 RIKPTRPFCL FNCYLDNPEK TAASEQGDFY ITGDRAHMDE DGYFWFVGRN DDVINSSSYR 490 500 510 520 530 540 IGPVEVESAL AEHPAVLESA VVSSPDPIRG EVVKAFIVLS PAYVSHDPEA LTRELQEHVK 550 560 570 TVTAPYKYPR KVAFISELPK TVSGKILRSK LRNQEWGR

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC078915 mRNA. Translation: AAH78915.1.
IPI	IPI00464802.
RefSeq	NP_001014184.3.
UniGene	Rn.35367
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000046025; ENSRNOP00000047613; ENSRNOG00000031211; Rattus norvegicus. [Genome view]
GeneID	361637.
KEGG	rno:361637.
UCSC	NM_001014162. rat.
Organism-specific databases
CTD	361637.
RGD	1309578. RGD1309578.
Phylogenomic databases
HOVERGEN	Q6AYT9.
Enzyme and pathway databases
BRENDA	6.2.1.2. 248.
Gene expression databases
Genevestigator	Q6AYT9.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	677039.

Entry information

Entry name

ACSM5_RAT

Accession

Primary (citable) accession number: Q6AYT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	September 13, 2004
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)