Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6AYT3 (RTCB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing ligase RtcB homolog

EC=6.5.1.3
Alternative name(s):
p55
Gene names
Name:Rtcb
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs By similarity. HAMAP-Rule MF_03144

Catalytic activity

ATP + (ribonucleotide)(n) + (ribonucleotide)(m) = AMP + diphosphate + (ribonucleotide)(n+m). HAMAP-Rule MF_03144

Subunit structure

Catalytic component of the tRNA-splicing ligase complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_03144.

Sequence similarities

Belongs to the RtcB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 505505tRNA-splicing ligase RtcB homolog HAMAP-Rule MF_03144
PRO_0000255245

Sites

Metal binding1221Zinc Potential
Metal binding2271Zinc Potential
Metal binding2591Zinc Potential

Experimental info

Sequence conflict3161Y → C in AAS78621. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6AYT3 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: 3488A9BF91460480

FASTA50555,249
        10         20         30         40         50         60 
MSRNYNDELQ FLDKINKNCW RIKKGFVPNM QVEGVFYVND ALEKLMFEEL RNACRGGGVG 

        70         80         90        100        110        120 
GFLPAMKQIG NVAALPGIVH RSIGLPDVHS GYGFAIGNMA AFDMNDPEAV VSPGGVGFDI 

       130        140        150        160        170        180 
NCGVRLLRTN LDESDVQPVK EQLAQAMFDH IPVGVGSKGV IPMNAKDLEE ALEMGVDWSL 

       190        200        210        220        230        240 
REGYAWAEDK EHCEEYGRML QADPNKVSPR AKKRGLPQLG TLGAGNHYAE IQVVDEIFNE 

       250        260        270        280        290        300 
YAAKKMGIDH KGQVCVMIHS GSRGLGHQVA TDALVAMEKA MKRDKIIVND RQLACARIAS 

       310        320        330        340        350        360 
PEGQDYLKGM AAAGNYAWVN RSSMTFLTRQ AFAKVFNTTP DDLDLHVIYD VSHNIAKVEQ 

       370        380        390        400        410        420 
HVVDGKERTL LVHRKGSTRA FPPHHPLIAV DYQLTGQPVL IGGTMGTCSY VLTGTEQGMT 

       430        440        450        460        470        480 
ETFGTTCHGA GRALSRAKSR RNLDFQDVLD KLADMGIAIR VASPKLVMEE APESYKNVTD 

       490        500 
VVNTCHDAGI SKKAIKLRPI AVIKG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the rat P55 gene."
Ding N.-Z., He M., He C.-Q., Chen J.G.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY572415 mRNA. Translation: AAS78621.1.
BC078924 mRNA. Translation: AAH78924.1.
RefSeqNP_997497.1. NM_207614.1.
UniGeneRn.11875.

3D structure databases

ProteinModelPortalQ6AYT3.
SMRQ6AYT3. Positions 12-505.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid263779. 2 interactions.
IntActQ6AYT3. 1 interaction.
MINTMINT-4576391.
STRING10116.ENSRNOP00000006570.

PTM databases

PhosphoSiteQ6AYT3.

Proteomic databases

PaxDbQ6AYT3.
PRIDEQ6AYT3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000006570; ENSRNOP00000006570; ENSRNOG00000004813.
GeneID362855.
KEGGrno:362855.
UCSCRGD:1303261. rat.

Organism-specific databases

CTD51493.
RGD1303261. Rtcb.

Phylogenomic databases

eggNOGCOG1690.
GeneTreeENSGT00390000015260.
HOVERGENHBG081383.
InParanoidQ6AYT3.
KOK14415.
OMAGRMLNAD.
OrthoDBEOG7DRJ2Q.
PhylomeDBQ6AYT3.
TreeFamTF314404.

Gene expression databases

GenevestigatorQ6AYT3.

Family and domain databases

HAMAPMF_03144. RtcB_euk.
InterProIPR001233. RtcB.
IPR027513. RtcB_euk.
[Graphical view]
PANTHERPTHR11118. PTHR11118. 1 hit.
PfamPF01139. RtcB. 1 hit.
[Graphical view]
SUPFAMSSF103365. SSF103365. 1 hit.
PROSITEPS01288. UPF0027. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio681535.
PROQ6AYT3.

Entry information

Entry nameRTCB_RAT
AccessionPrimary (citable) accession number: Q6AYT3
Secondary accession number(s): Q6PX76
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 13, 2004
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families