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Q6AYS7

- ACY1A_RAT

UniProt

Q6AYS7 - ACY1A_RAT

Protein

Aminoacylase-1A

Gene

Acy1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (13 Sep 2004)
      Previous versions | rss
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    Functioni

    Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).By similarity

    Catalytic activityi

    An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
    An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi80 – 801Zinc 1By similarity
    Active sitei82 – 821By similarity
    Metal bindingi113 – 1131Zinc 1By similarity
    Metal bindingi113 – 1131Zinc 2By similarity
    Active sitei147 – 1471Proton acceptorBy similarity
    Metal bindingi148 – 1481Zinc 2By similarity
    Metal bindingi175 – 1751Zinc 1By similarity
    Metal bindingi373 – 3731Zinc 2By similarity

    GO - Molecular functioni

    1. aminoacylase activity Source: RGD
    2. metal ion binding Source: UniProtKB-KW
    3. metallopeptidase activity Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro
    2. protein catabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.1.14. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminoacylase-1A (EC:3.5.1.14)
    Short name:
    ACY-1A
    Alternative name(s):
    ACY IA
    N-acyl-L-amino-acid amidohydrolase
    Gene namesi
    Name:Acy1a
    Synonyms:Acy1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi2030. Acy1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408Aminoacylase-1APRO_0000274009Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PaxDbiQ6AYS7.
    PRIDEiQ6AYS7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6AYS7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015852.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6AYS7.
    SMRiQ6AYS7. Positions 7-198, 321-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Phylogenomic databases

    eggNOGiCOG0624.
    GeneTreeiENSGT00730000111049.
    HOGENOMiHOG000021196.
    HOVERGENiHBG000982.
    InParanoidiQ6AYS7.
    KOiK14677.
    OMAiKGMELFV.
    OrthoDBiEOG7N8ZVZ.
    TreeFamiTF313693.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036696. ACY-1. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6AYS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTKGPESEH PSVTLFRQYL RICTVQPNPD YGSAVTFLEE RARQLGLSCQ    50
    KIEVAPGYVI TVLTWPGTNP LLHSILLNSH TDVVPVFKEH WHHDPFEAFK 100
    DSEGYIYARG AQDMKSVSIQ YLEAVRRLKS EGHRFPRTIH MTFVPDEEVG 150
    GHKGMELFVK RPEFQALRAG FALDEGLANP TDAFTVFYSE RSPWWIRVTS 200
    TGKPGHASRF IEDTAAEKLH KVVNSILAFR EKERQRLQAN PHLKEGAVTS 250
    VNLTKLEGGV AYNVVPATMS ACFDFRVAPD VDMKAFEKQL QSWCQEAGEG 300
    VTFEFAQKFT EPRMTPTDDT DPWWAAFSGA CKEMNLTLEP EIFPAATDSR 350
    YIRAVGIPAL GFSPMNRTPV LLHDHNERLH EAVFLRGVDI YTRLVAALAS 400
    VPALPGES 408
    Length:408
    Mass (Da):45,804
    Last modified:September 13, 2004 - v1
    Checksum:iCC9B4505927D432A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 86TKGPES → SKVPEE in AAS90690. (PubMed:15253876)Curated
    Sequence conflicti132 – 1321G → S in AAS90690. (PubMed:15253876)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY580164 mRNA. Translation: AAS90690.1.
    BC078930 mRNA. Translation: AAH78930.1.
    RefSeqiNP_001005383.1. NM_001005383.1.
    XP_006243777.1. XM_006243715.1.
    XP_006243778.1. XM_006243716.1.
    UniGeneiRn.3679.

    Genome annotation databases

    EnsembliENSRNOT00000015851; ENSRNOP00000015852; ENSRNOG00000011189.
    GeneIDi300981.
    KEGGirno:300981.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY580164 mRNA. Translation: AAS90690.1 .
    BC078930 mRNA. Translation: AAH78930.1 .
    RefSeqi NP_001005383.1. NM_001005383.1.
    XP_006243777.1. XM_006243715.1.
    XP_006243778.1. XM_006243716.1.
    UniGenei Rn.3679.

    3D structure databases

    ProteinModelPortali Q6AYS7.
    SMRi Q6AYS7. Positions 7-198, 321-408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015852.

    Proteomic databases

    PaxDbi Q6AYS7.
    PRIDEi Q6AYS7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015851 ; ENSRNOP00000015852 ; ENSRNOG00000011189 .
    GeneIDi 300981.
    KEGGi rno:300981.

    Organism-specific databases

    CTDi 95.
    RGDi 2030. Acy1.

    Phylogenomic databases

    eggNOGi COG0624.
    GeneTreei ENSGT00730000111049.
    HOGENOMi HOG000021196.
    HOVERGENi HBG000982.
    InParanoidi Q6AYS7.
    KOi K14677.
    OMAi KGMELFV.
    OrthoDBi EOG7N8ZVZ.
    TreeFami TF313693.

    Enzyme and pathway databases

    BRENDAi 3.5.1.14. 5301.

    Miscellaneous databases

    NextBioi 647830.

    Gene expression databases

    Genevestigatori Q6AYS7.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036696. ACY-1. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The rat kidney acylase 1. Evidence for a new cDNA form and comparisons with the porcine intestinal enzyme."
      Perrier J., Durand A., Giardina T., Puigserver A.
      Comp. Biochem. Physiol. 138B:277-283(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 89-100; 116-126; 169-190 AND 368-378, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiACY1A_RAT
    AccessioniPrimary (citable) accession number: Q6AYS7
    Secondary accession number(s): Q6PTT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: September 13, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3