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Protein

Aminoacylase-1A

Gene

Acy1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).By similarity

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi80Zinc 1By similarity1
Active sitei82By similarity1
Metal bindingi113Zinc 1By similarity1
Metal bindingi113Zinc 2By similarity1
Active sitei147Proton acceptorBy similarity1
Metal bindingi148Zinc 2By similarity1
Metal bindingi175Zinc 1By similarity1
Metal bindingi373Zinc 2By similarity1

GO - Molecular functioni

  • aminoacylase activity Source: RGD
  • metallodipeptidase activity Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • cellular amino acid metabolic process Source: InterPro
  • peptide catabolic process Source: GO_Central
  • protein catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.14. 5301.
ReactomeiR-RNO-5423646. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacylase-1A (EC:3.5.1.14)
Short name:
ACY-1A
Alternative name(s):
ACY IA
N-acyl-L-amino-acid amidohydrolase
Gene namesi
Name:Acy1a
Synonyms:Acy1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2030. Acy1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002740091 – 408Aminoacylase-1AAdd BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei408PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AYS7.
PRIDEiQ6AYS7.

PTM databases

iPTMnetiQ6AYS7.
PhosphoSitePlusiQ6AYS7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000011189.
GenevisibleiQ6AYS7. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ6AYS7. 1 interactor.
STRINGi10116.ENSRNOP00000015852.

Structurei

3D structure databases

ProteinModelPortaliQ6AYS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiKOG2275. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00840000129850.
HOGENOMiHOG000021196.
HOVERGENiHBG000982.
InParanoidiQ6AYS7.
KOiK14677.
OMAiEPHKWDH.
OrthoDBiEOG091G0A7U.
TreeFamiTF313693.

Family and domain databases

CDDicd05646. M20_AcylaseI_like. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF036696. ACY-1. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AYS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKGPESEH PSVTLFRQYL RICTVQPNPD YGSAVTFLEE RARQLGLSCQ
60 70 80 90 100
KIEVAPGYVI TVLTWPGTNP LLHSILLNSH TDVVPVFKEH WHHDPFEAFK
110 120 130 140 150
DSEGYIYARG AQDMKSVSIQ YLEAVRRLKS EGHRFPRTIH MTFVPDEEVG
160 170 180 190 200
GHKGMELFVK RPEFQALRAG FALDEGLANP TDAFTVFYSE RSPWWIRVTS
210 220 230 240 250
TGKPGHASRF IEDTAAEKLH KVVNSILAFR EKERQRLQAN PHLKEGAVTS
260 270 280 290 300
VNLTKLEGGV AYNVVPATMS ACFDFRVAPD VDMKAFEKQL QSWCQEAGEG
310 320 330 340 350
VTFEFAQKFT EPRMTPTDDT DPWWAAFSGA CKEMNLTLEP EIFPAATDSR
360 370 380 390 400
YIRAVGIPAL GFSPMNRTPV LLHDHNERLH EAVFLRGVDI YTRLVAALAS

VPALPGES
Length:408
Mass (Da):45,804
Last modified:September 13, 2004 - v1
Checksum:iCC9B4505927D432A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 8TKGPES → SKVPEE in AAS90690 (PubMed:15253876).Curated6
Sequence conflicti132G → S in AAS90690 (PubMed:15253876).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY580164 mRNA. Translation: AAS90690.1.
BC078930 mRNA. Translation: AAH78930.1.
RefSeqiNP_001005383.1. NM_001005383.1.
XP_006243777.1. XM_006243715.3.
XP_006243778.1. XM_006243716.3.
UniGeneiRn.3679.

Genome annotation databases

EnsembliENSRNOT00000015851; ENSRNOP00000015852; ENSRNOG00000011189.
GeneIDi300981.
KEGGirno:300981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY580164 mRNA. Translation: AAS90690.1.
BC078930 mRNA. Translation: AAH78930.1.
RefSeqiNP_001005383.1. NM_001005383.1.
XP_006243777.1. XM_006243715.3.
XP_006243778.1. XM_006243716.3.
UniGeneiRn.3679.

3D structure databases

ProteinModelPortaliQ6AYS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6AYS7. 1 interactor.
STRINGi10116.ENSRNOP00000015852.

PTM databases

iPTMnetiQ6AYS7.
PhosphoSitePlusiQ6AYS7.

Proteomic databases

PaxDbiQ6AYS7.
PRIDEiQ6AYS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015851; ENSRNOP00000015852; ENSRNOG00000011189.
GeneIDi300981.
KEGGirno:300981.

Organism-specific databases

CTDi95.
RGDi2030. Acy1.

Phylogenomic databases

eggNOGiKOG2275. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00840000129850.
HOGENOMiHOG000021196.
HOVERGENiHBG000982.
InParanoidiQ6AYS7.
KOiK14677.
OMAiEPHKWDH.
OrthoDBiEOG091G0A7U.
TreeFamiTF313693.

Enzyme and pathway databases

BRENDAi3.5.1.14. 5301.
ReactomeiR-RNO-5423646. Aflatoxin activation and detoxification.

Gene expression databases

BgeeiENSRNOG00000011189.
GenevisibleiQ6AYS7. RN.

Family and domain databases

CDDicd05646. M20_AcylaseI_like. 1 hit.
Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF036696. ACY-1. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACY1A_RAT
AccessioniPrimary (citable) accession number: Q6AYS7
Secondary accession number(s): Q6PTT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.