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Q6AYS7

- ACY1A_RAT

UniProt

Q6AYS7 - ACY1A_RAT

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Protein

Aminoacylase-1A

Gene

Acy1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).By similarity

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Binds 2 zinc ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi80 – 801Zinc 1By similarity
Active sitei82 – 821By similarity
Metal bindingi113 – 1131Zinc 1By similarity
Metal bindingi113 – 1131Zinc 2By similarity
Active sitei147 – 1471Proton acceptorBy similarity
Metal bindingi148 – 1481Zinc 2By similarity
Metal bindingi175 – 1751Zinc 1By similarity
Metal bindingi373 – 3731Zinc 2By similarity

GO - Molecular functioni

  1. aminoacylase activity Source: RGD
  2. metal ion binding Source: UniProtKB-KW
  3. metallopeptidase activity Source: InterPro

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
  2. protein catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.14. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacylase-1A (EC:3.5.1.14)
Short name:
ACY-1A
Alternative name(s):
ACY IA
N-acyl-L-amino-acid amidohydrolase
Gene namesi
Name:Acy1a
Synonyms:Acy1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi2030. Acy1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Aminoacylase-1APRO_0000274009Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ6AYS7.
PRIDEiQ6AYS7.

Expressioni

Gene expression databases

GenevestigatoriQ6AYS7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015852.

Structurei

3D structure databases

ProteinModelPortaliQ6AYS7.
SMRiQ6AYS7. Positions 7-198, 321-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiCOG0624.
GeneTreeiENSGT00730000111049.
HOGENOMiHOG000021196.
HOVERGENiHBG000982.
InParanoidiQ6AYS7.
KOiK14677.
OMAiKGMELFV.
OrthoDBiEOG7N8ZVZ.
TreeFamiTF313693.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF036696. ACY-1. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AYS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTKGPESEH PSVTLFRQYL RICTVQPNPD YGSAVTFLEE RARQLGLSCQ
60 70 80 90 100
KIEVAPGYVI TVLTWPGTNP LLHSILLNSH TDVVPVFKEH WHHDPFEAFK
110 120 130 140 150
DSEGYIYARG AQDMKSVSIQ YLEAVRRLKS EGHRFPRTIH MTFVPDEEVG
160 170 180 190 200
GHKGMELFVK RPEFQALRAG FALDEGLANP TDAFTVFYSE RSPWWIRVTS
210 220 230 240 250
TGKPGHASRF IEDTAAEKLH KVVNSILAFR EKERQRLQAN PHLKEGAVTS
260 270 280 290 300
VNLTKLEGGV AYNVVPATMS ACFDFRVAPD VDMKAFEKQL QSWCQEAGEG
310 320 330 340 350
VTFEFAQKFT EPRMTPTDDT DPWWAAFSGA CKEMNLTLEP EIFPAATDSR
360 370 380 390 400
YIRAVGIPAL GFSPMNRTPV LLHDHNERLH EAVFLRGVDI YTRLVAALAS

VPALPGES
Length:408
Mass (Da):45,804
Last modified:September 13, 2004 - v1
Checksum:iCC9B4505927D432A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 86TKGPES → SKVPEE in AAS90690. (PubMed:15253876)Curated
Sequence conflicti132 – 1321G → S in AAS90690. (PubMed:15253876)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY580164 mRNA. Translation: AAS90690.1.
BC078930 mRNA. Translation: AAH78930.1.
RefSeqiNP_001005383.1. NM_001005383.1.
XP_006243777.1. XM_006243715.2.
XP_006243778.1. XM_006243716.2.
UniGeneiRn.3679.

Genome annotation databases

EnsembliENSRNOT00000015851; ENSRNOP00000015852; ENSRNOG00000011189.
GeneIDi300981.
KEGGirno:300981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY580164 mRNA. Translation: AAS90690.1 .
BC078930 mRNA. Translation: AAH78930.1 .
RefSeqi NP_001005383.1. NM_001005383.1.
XP_006243777.1. XM_006243715.2.
XP_006243778.1. XM_006243716.2.
UniGenei Rn.3679.

3D structure databases

ProteinModelPortali Q6AYS7.
SMRi Q6AYS7. Positions 7-198, 321-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015852.

Proteomic databases

PaxDbi Q6AYS7.
PRIDEi Q6AYS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015851 ; ENSRNOP00000015852 ; ENSRNOG00000011189 .
GeneIDi 300981.
KEGGi rno:300981.

Organism-specific databases

CTDi 95.
RGDi 2030. Acy1.

Phylogenomic databases

eggNOGi COG0624.
GeneTreei ENSGT00730000111049.
HOGENOMi HOG000021196.
HOVERGENi HBG000982.
InParanoidi Q6AYS7.
KOi K14677.
OMAi KGMELFV.
OrthoDBi EOG7N8ZVZ.
TreeFami TF313693.

Enzyme and pathway databases

BRENDAi 3.5.1.14. 5301.

Miscellaneous databases

NextBioi 647830.

Gene expression databases

Genevestigatori Q6AYS7.

Family and domain databases

Gene3Di 3.30.70.360. 1 hit.
InterProi IPR001261. ArgE/DapE_CS.
IPR010159. N-acyl_aa_amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view ]
Pfami PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view ]
PIRSFi PIRSF036696. ACY-1. 1 hit.
SUPFAMi SSF55031. SSF55031. 1 hit.
TIGRFAMsi TIGR01880. Ac-peptdase-euk. 1 hit.
PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The rat kidney acylase 1. Evidence for a new cDNA form and comparisons with the porcine intestinal enzyme."
    Perrier J., Durand A., Giardina T., Puigserver A.
    Comp. Biochem. Physiol. 138B:277-283(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 89-100; 116-126; 169-190 AND 368-378, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiACY1A_RAT
AccessioniPrimary (citable) accession number: Q6AYS7
Secondary accession number(s): Q6PTT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 13, 2004
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3