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Protein

Phenylalanine--tRNA ligase, mitochondrial

Gene

Fars2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei287 – 2871Substrate; via carbonyl oxygenBy similarity
Binding sitei312 – 3121Substrate; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase, mitochondrial (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase
Short name:
PheRS
Gene namesi
Name:Fars2
Synonyms:Fars1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1309416. Fars2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3757.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 472Phenylalanine--tRNA ligase, mitochondrialPRO_0000035815
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei202 – 2021N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6AYQ3.
PRIDEiQ6AYQ3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016135.
ExpressionAtlasiQ6AYQ3. baseline and differential.
GenevisibleiQ6AYQ3. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021660.

Structurei

3D structure databases

ProteinModelPortaliQ6AYQ3.
SMRiQ6AYQ3. Positions 47-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini379 – 47193FDX-ACBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1604Substrate bindingBy similarity
Regioni186 – 1883Substrate bindingBy similarity
Regioni193 – 1953Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 FDX-ACB domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2783. Eukaryota.
COG0016. LUCA.
COG0072. LUCA.
GeneTreeiENSGT00530000063467.
HOGENOMiHOG000165163.
HOVERGENiHBG082540.
InParanoidiQ6AYQ3.
KOiK01889.
OMAiTYFPFTQ.
OrthoDBiEOG091G07OD.
PhylomeDBiQ6AYQ3.
TreeFamiTF105798.

Family and domain databases

Gene3Di3.30.70.380. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004530. Phe-tRNA-synth_IIc_mito.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
[Graphical view]
PfamiPF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 2 hits.
[Graphical view]
SMARTiSM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF54991. SSF54991. 1 hit.
TIGRFAMsiTIGR00469. pheS_mito. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS51447. FDX_ACB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCLAFIRAA HEHLYLVRKV SHVCRCHQHR AWGSRPAASQ FAVQGAPGRV
60 70 80 90 100
LELLGKSYPQ DDHTNLTQKV LSKVGRNLHN QKFHPLWLIK ERVKEHFYQQ
110 120 130 140 150
YMGRSRTPLF SVYDQLSPVV TTWQNFDSLL IPADHPSRKK GDNYYLNRGH
160 170 180 190 200
MLRAHTSAHQ WDLLHAGLNA FLVVGDVYRR DQIDSQHYPV FHQLEGVRLF
210 220 230 240 250
SKHELFAGVK DGESLQLFEE SSRSAHKQET HTMEAVKLVE FDLKQVLTRL
260 270 280 290 300
VTHLFGDGLE VRWVDCYFPF THPSFEMEIN FRGEWLEVLG CGVMEQQLVN
310 320 330 340 350
SAGAQDRIGW AFGLGLERLA MVLYDIPDIR LFWSEDERFL KQFRLSDINQ
360 370 380 390 400
SVKFQRWFFQ EERATGIQRK MGRQLCPFSK YPAVFNDISF WLPSENYTEN
410 420 430 440 450
DFYDIVRTVG GDLVEKVDLI DKFEHPKTHR TSHCYRITYR HMERTLSQRE
460 470
VSSVHQAVQE AAVQLLGVEG RF
Length:472
Mass (Da):55,165
Last modified:September 13, 2004 - v1
Checksum:iF0C90187062FBA20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078956 mRNA. Translation: AAH78956.1.
RefSeqiNP_001013157.1. NM_001013139.1.
UniGeneiRn.8968.

Genome annotation databases

EnsembliENSRNOT00000021660; ENSRNOP00000021660; ENSRNOG00000016135.
GeneIDi306879.
KEGGirno:306879.
UCSCiRGD:1309416. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078956 mRNA. Translation: AAH78956.1.
RefSeqiNP_001013157.1. NM_001013139.1.
UniGeneiRn.8968.

3D structure databases

ProteinModelPortaliQ6AYQ3.
SMRiQ6AYQ3. Positions 47-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021660.

Chemistry

ChEMBLiCHEMBL3757.

Proteomic databases

PaxDbiQ6AYQ3.
PRIDEiQ6AYQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021660; ENSRNOP00000021660; ENSRNOG00000016135.
GeneIDi306879.
KEGGirno:306879.
UCSCiRGD:1309416. rat.

Organism-specific databases

CTDi10667.
RGDi1309416. Fars2.

Phylogenomic databases

eggNOGiKOG2783. Eukaryota.
COG0016. LUCA.
COG0072. LUCA.
GeneTreeiENSGT00530000063467.
HOGENOMiHOG000165163.
HOVERGENiHBG082540.
InParanoidiQ6AYQ3.
KOiK01889.
OMAiTYFPFTQ.
OrthoDBiEOG091G07OD.
PhylomeDBiQ6AYQ3.
TreeFamiTF105798.

Miscellaneous databases

PROiQ6AYQ3.

Gene expression databases

BgeeiENSRNOG00000016135.
ExpressionAtlasiQ6AYQ3. baseline and differential.
GenevisibleiQ6AYQ3. RN.

Family and domain databases

Gene3Di3.30.70.380. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004530. Phe-tRNA-synth_IIc_mito.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
[Graphical view]
PfamiPF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 2 hits.
[Graphical view]
SMARTiSM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF54991. SSF54991. 1 hit.
TIGRFAMsiTIGR00469. pheS_mito. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS51447. FDX_ACB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFM_RAT
AccessioniPrimary (citable) accession number: Q6AYQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.