Q6AYQ2 (AK1CL_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldo-keto reductase family 1 member C21
EC=1.1.1.-

Alternative name(s):
17-alpha-hydroxysteroid dehydrogenase
Short name=17-alpha-HSD
3-alpha-hydroxysteroid dehydrogenase

Gene names

Name:

Akr1c21

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into epitestosterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha-hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3-ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17-alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Aldo-keto reductase family 1 member C21		PRO_0000326223
Regions
	Nucleotide binding	13 – 24	12	NADP By similarity
	Nucleotide binding	166 – 167	2	NADP By similarity
	Nucleotide binding	216 – 224	9	NADP By similarity
	Nucleotide binding	270 – 279	10	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	31	1	Substrate By similarity
	Binding site	50	1	NADP By similarity
	Binding site	117	1	Substrate By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	88	1	Phosphothreonine By similarity
	Modified residue	89	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AYQ2 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 53909644919456D1	FASTA	318	36,135
	10 20 30 40 50 60 MNSKCHCVKL NDGHFIPVLG FGTAMPSELP KSKAKEVTKI AIDAGFHHFD SAFVYNTEDH 70 80 90 100 110 120 VGEAIREKIA NGTTRREDIF YTSKLWCTSL HPELVRSSLE CSLKKLQLDY VDLYLIHFPM 130 140 150 160 170 180 ALKPGDENFP VDEHGKLLFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEKILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHLYLNQM KLLDFCKTNG IILVAYGVLG TQRYNGWVDQ NSPVLLNEPV 250 260 270 280 290 300 LSSMAKKYNQ TPALIALRHQ LQRGIVVLNT SLKEERIKEN MKLSPEDMKV LDDLNRNLRY 310 IAGGIFEGHP NFPFLDEY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC078957 mRNA. Translation: AAH78957.1.
IPI	IPI00555261.
RefSeq	NP_001013075.1. NM_001013057.1.
UniGene	Rn.139229.
3D structure databases
HSSP	HSSP built from PDB template 1XF0 based on UniProtKB P42330.
ProteinModelPortal	Q6AYQ2.
SMR	Q6AYQ2. Positions 1-318.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AYQ2.
PTM databases
PhosphoSite	Q6AYQ2.
Proteomic databases
PRIDE	Q6AYQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000051342; ENSRNOP00000045355; ENSRNOG00000029844.
GeneID	291283.
KEGG	rno:291283.
UCSC	NM_001013057. rat.
Organism-specific databases
CTD	1646.
RGD	1311841. Akr1c21.
Phylogenomic databases
eggNOG	roNOG15221.
GeneTree	ENSGT00550000074107.
HOVERGEN	HBG000020.
InParanoid	Q6AYQ2.
OrthoDB	EOG4QJRNQ.
PhylomeDB	Q6AYQ2.
Gene expression databases
Genevestigator	Q6AYQ2.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. False negative. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	632346.

Entry information

Entry name

AK1CL_RAT

Accession

Primary (citable) accession number: Q6AYQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 13, 2004
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program