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Protein

Aldo-keto reductase family 1 member C21

Gene

Akr1c21

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha-hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3-ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17-alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids (By similarity).By similarity

Catalytic activityi

Androsterone + NAD(P)+ = 5-alpha-androstane-3,17-dione + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateBy similarity1
Binding sitei50NADPBy similarity1
Active sitei55Proton donorBy similarity1
Sitei84Lowers pKa of active site TyrBy similarity1
Binding sitei117SubstrateBy similarity1
Binding sitei190NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 24NADPBy similarity5
Nucleotide bindingi166 – 167NADPBy similarity2
Nucleotide bindingi216 – 224NADPBy similarity9
Nucleotide bindingi270 – 280NADPBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-RNO-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-5365859. RA biosynthesis pathway.
R-RNO-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldo-keto reductase family 1 member C21 (EC:1.1.1.-)
Alternative name(s):
17-alpha-hydroxysteroid dehydrogenase
Short name:
17-alpha-HSD
3(or 17)-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.209)
3-alpha-hydroxysteroid dehydrogenase
Gene namesi
Name:Akr1c21
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1311841. Akr1c21.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003262231 – 318Aldo-keto reductase family 1 member C21Add BLAST318

Proteomic databases

PaxDbiQ6AYQ2.
PRIDEiQ6AYQ2.

PTM databases

iPTMnetiQ6AYQ2.
PhosphoSitePlusiQ6AYQ2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029844.
ExpressionAtlasiQ6AYQ2. differential.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045355.

Structurei

3D structure databases

ProteinModelPortaliQ6AYQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ6AYQ2.
PhylomeDBiQ6AYQ2.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AYQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSKCHCVKL NDGHFIPVLG FGTAMPSELP KSKAKEVTKI AIDAGFHHFD
60 70 80 90 100
SAFVYNTEDH VGEAIREKIA NGTTRREDIF YTSKLWCTSL HPELVRSSLE
110 120 130 140 150
CSLKKLQLDY VDLYLIHFPM ALKPGDENFP VDEHGKLLFD TVDLCATWEA
160 170 180 190 200
MEKCKDAGLA KSIGVSNFNR RQLEKILNKP GLKYKPVCNQ VECHLYLNQM
210 220 230 240 250
KLLDFCKTNG IILVAYGVLG TQRYNGWVDQ NSPVLLNEPV LSSMAKKYNQ
260 270 280 290 300
TPALIALRHQ LQRGIVVLNT SLKEERIKEN MKLSPEDMKV LDDLNRNLRY
310
IAGGIFEGHP NFPFLDEY
Length:318
Mass (Da):36,135
Last modified:September 13, 2004 - v1
Checksum:i53909644919456D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078957 mRNA. Translation: AAH78957.1.
RefSeqiNP_001013075.1. NM_001013057.1.
UniGeneiRn.139229.

Genome annotation databases

EnsembliENSRNOT00000051342; ENSRNOP00000045355; ENSRNOG00000029844.
GeneIDi291283.
KEGGirno:291283.
UCSCiRGD:1311841. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078957 mRNA. Translation: AAH78957.1.
RefSeqiNP_001013075.1. NM_001013057.1.
UniGeneiRn.139229.

3D structure databases

ProteinModelPortaliQ6AYQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045355.

PTM databases

iPTMnetiQ6AYQ2.
PhosphoSitePlusiQ6AYQ2.

Proteomic databases

PaxDbiQ6AYQ2.
PRIDEiQ6AYQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051342; ENSRNOP00000045355; ENSRNOG00000029844.
GeneIDi291283.
KEGGirno:291283.
UCSCiRGD:1311841. rat.

Organism-specific databases

CTDi1646.
RGDi1311841. Akr1c21.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ6AYQ2.
PhylomeDBiQ6AYQ2.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-RNO-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-5365859. RA biosynthesis pathway.
R-RNO-975634. Retinoid metabolism and transport.

Miscellaneous databases

PROiQ6AYQ2.

Gene expression databases

BgeeiENSRNOG00000029844.
ExpressionAtlasiQ6AYQ2. differential.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAK1CL_RAT
AccessioniPrimary (citable) accession number: Q6AYQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.