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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

Nt5c3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).By similarity

Catalytic activityi

N(7)-methyl-GMP + H2O = N(7)-methyl-guanosine + phosphate.By similarity
CMP + H2O = cytidine + phosphate.By similarity
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411NucleophileBy similarity
Metal bindingi41 – 411MagnesiumBy similarity
Active sitei43 – 431Proton donorBy similarity
Metal bindingi43 – 431Magnesium; via carbonyl oxygenBy similarity
Binding sitei88 – 881CMPBy similarity
Binding sitei88 – 881N(7)-methyl-GMPBy similarity
Binding sitei205 – 2051SubstrateBy similarity
Metal bindingi230 – 2301MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-429958. mRNA decay by 3' to 5' exoribonuclease.
SABIO-RKQ6AYP7.

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidaseBy similarity (EC:3.1.3.91By similarity)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like proteinBy similarity (EC:3.1.3.5By similarity)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:Nt5c3b
Synonyms:Nt5c3l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1359229. Nt5c3b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3003007-methylguanosine phosphate-specific 5'-nucleotidasePRO_0000328950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6AYP7.
PRIDEiQ6AYP7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016475.
ExpressionAtlasiQ6AYP7. baseline.
GenevisibleiQ6AYP7. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022430.

Structurei

3D structure databases

ProteinModelPortaliQ6AYP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 1572Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOGENOMiHOG000244931.
HOVERGENiHBG059750.
InParanoidiQ6AYP7.
KOiK01081.
OMAiDPHRTVK.
TreeFamiTF314663.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AYP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEVSSLMK ATVLMRQPGR VQEIVSALRR GGGDRLQVIS DFDMTLSRFA
60 70 80 90 100
YNGQRCPSSH NILDNSKIIS EDCRKELTEL FHHYYPIEID PHRTIKEKLP
110 120 130 140 150
HMVQWWSKAH SLLCQQRIQK FQIAQVVGES TAMLREGYKM FFDTLYHNNI
160 170 180 190 200
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFSEDG FLKGFKGQLI
210 220 230 240 250
HTYNKNSSVC ENSSYFQQLR NKTNIILLGD SIGDLTMADG VPGVQNILKI
260 270 280 290 300
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILR QGDCVELQGS
Length:300
Mass (Da):34,536
Last modified:April 3, 2013 - v2
Checksum:i73AD8E974981E20A
GO

Sequence cautioni

The sequence AAH78963 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078963 mRNA. Translation: AAH78963.1. Different initiation.
RefSeqiNP_001007724.2. NM_001007723.2.
XP_008766305.1. XM_008768083.1.
UniGeneiRn.24114.

Genome annotation databases

EnsembliENSRNOT00000022430; ENSRNOP00000022430; ENSRNOG00000016475.
ENSRNOT00000080006; ENSRNOP00000069295; ENSRNOG00000016475.
GeneIDi360629.
KEGGirno:360629.
UCSCiRGD:1359229. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078963 mRNA. Translation: AAH78963.1. Different initiation.
RefSeqiNP_001007724.2. NM_001007723.2.
XP_008766305.1. XM_008768083.1.
UniGeneiRn.24114.

3D structure databases

ProteinModelPortaliQ6AYP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022430.

Proteomic databases

PaxDbiQ6AYP7.
PRIDEiQ6AYP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022430; ENSRNOP00000022430; ENSRNOG00000016475.
ENSRNOT00000080006; ENSRNOP00000069295; ENSRNOG00000016475.
GeneIDi360629.
KEGGirno:360629.
UCSCiRGD:1359229. rat.

Organism-specific databases

CTDi115024.
RGDi1359229. Nt5c3b.

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOGENOMiHOG000244931.
HOVERGENiHBG059750.
InParanoidiQ6AYP7.
KOiK01081.
OMAiDPHRTVK.
TreeFamiTF314663.

Enzyme and pathway databases

ReactomeiR-RNO-429958. mRNA decay by 3' to 5' exoribonuclease.
SABIO-RKQ6AYP7.

Miscellaneous databases

PROiQ6AYP7.

Gene expression databases

BgeeiENSRNOG00000016475.
ExpressionAtlasiQ6AYP7. baseline.
GenevisibleiQ6AYP7. RN.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei5NT3B_RAT
AccessioniPrimary (citable) accession number: Q6AYP7
Secondary accession number(s): F8WG43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: September 7, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.