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Protein

Cell adhesion molecule 1

Gene

Cadm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates homophilic cell-cell adhesion in a Ca2+-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca2+-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-418990. Adherens junctions interactions.
R-RNO-420597. Nectin/Necl trans heterodimerization.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell adhesion molecule 1Curated
Alternative name(s):
Synaptic cell adhesion moleculeCurated
Short name:
SynCAMCurated
Tumor suppressor in lung cancer 1 homolog1 Publication
Short name:
TSLC-11 Publication
Gene namesi
Name:Cadm1Imported
Synonyms:SynCam11 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1310999. Cadm1.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity
  • Cell junctionsynapse By similarity
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication

  • Note: Localized to the basolateral plasma membrane of epithelial cells in gall bladder.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini48 – 408ExtracellularBy similarityAdd BLAST361
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • cell-cell junction Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 47Sequence analysisAdd BLAST47
ChainiPRO_500427076348 – 476Cell adhesion molecule 1Sequence analysisAdd BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 127PROSITE-ProRule annotation
Glycosylationi70N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi104N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi116N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi168N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi169 ↔ 223PROSITE-ProRule annotation
Disulfide bondi270 ↔ 316PROSITE-ProRule annotation
Glycosylationi304N-linked (GlcNAc...) asparagine; atypicalBy similarity1
Glycosylationi307N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi311N-linked (GlcNAc...) asparagineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity
Glycosylation at Asn-70 and Asn-104 promotes adhesive binding and synapse induction.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6AYP5.

PTM databases

iPTMnetiQ6AYP5.
PhosphoSitePlusiQ6AYP5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018778.

Interactioni

Subunit structurei

Homodimer. Interacts with CRTAM. Interacts (via C-terminus) with EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP2, MPP3 and MPP6. Interacts with FARP1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041169.

Structurei

3D structure databases

ProteinModelPortaliQ6AYP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 142Ig-like V-typePROSITE-ProRule annotationAdd BLAST95
Domaini147 – 241Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST95
Domaini246 – 332Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST87

Domaini

The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.By similarity

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH4H. Eukaryota.
ENOG41116SB. LUCA.
HOGENOMiHOG000036057.
HOVERGENiHBG057086.
KOiK06781.
OrthoDBiEOG091G0BHE.
PhylomeDBiQ6AYP5.
TreeFamiTF334317.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiView protein in InterPro
IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR003585. Neurexin-like.
PfamiView protein in Pfam
PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00294. 4.1m. 1 hit.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AYP5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPVLPSGS QCAAAAAVAA AAAPPGLRLR LLLLLLSAAA LIPTGDGQNL
60 70 80 90 100
FTKDVTVIEG EVATISCQVN KSDDSVIQLL NPNRQTIYFR DFRPLKDSRF
110 120 130 140 150
QLLNFSSSEL KVSLTNVSIS DEGRYFCQLY TDPPQESYTT ITVLVPPRNL
160 170 180 190 200
MIDIQKDTAV EGEEIEVNCT AMASKPATTI RWFKGNKELK GKSEVEEWSD
210 220 230 240 250
MYTVTSQLML KVHKEDDGVP VICQVEHPAV TGNLQTQRYL EVQYKPQVQI
260 270 280 290 300
QMTYPLQGLT REGDAFELTC EATGKPQPVM VTWVRVDDEM PQHAVLSGPN
310 320 330 340 350
LFINNLNKTD NGTYRCEASN TVGKAHSDYM LYVYDPPTTI PPPTTTTTTT
360 370 380 390 400
TTTTTTTTIL TIITDTTATT EPAVHGLTQL PNSAEELDSE DLSDSRAGEE
410 420 430 440 450
GAIGAVDHAV IGGVVAVVVF AMLCLLIILG RYFARHKGTY FTHEAKGADD
460 470
AADADTAIIN AEGGQNNSEE KKEYFI
Length:476
Mass (Da):51,854
Last modified:September 13, 2004 - v1
Checksum:i486A43D37082C8FE
GO
Isoform 2 (identifier: Q6AYP5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     335-476: DPPTTIPPPT...NSEEKKEYFI → GT

Show »
Length:336
Mass (Da):37,148
Checksum:i51ED251D22DAFB63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4P → T in BAE97778 (PubMed:16814249).Curated1
Sequence conflicti150L → M in BAE97778 (PubMed:16814249).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058825335 – 476DPPTT…KEYFI → GT in isoform 2. Add BLAST142

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB257091 mRNA. Translation: BAE97778.1.
AABR07070099 Genomic DNA. No translation available.
BC078966 mRNA. Translation: AAH78966.1.
RefSeqiNP_001012201.1. NM_001012201.1. [Q6AYP5-1]
UniGeneiRn.19928.

Genome annotation databases

GeneIDi363058.
KEGGirno:363058.
UCSCiRGD:1310999. rat. [Q6AYP5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCADM1_RAT
AccessioniPrimary (citable) accession number: Q6AYP5
Secondary accession number(s): F7EYW4, Q0WXX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
Last sequence update: September 13, 2004
Last modified: June 7, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families