Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinetochore protein Nuf2

Gene

Nuf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:Nuf2
Synonyms:Cdca1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1307952. Nuf2.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore By similarity

  • Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498151 – 464Kinetochore protein Nuf2Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei171PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYL9.
PRIDEiQ6AYL9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002711.
GenevisibleiQ6AYL9. RN.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003650.

Structurei

3D structure databases

ProteinModelPortaliQ6AYL9.
SMRiQ6AYL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80By similarityAdd BLAST385
Regioni386 – 464Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili148 – 201Sequence analysisAdd BLAST54
Coiled coili249 – 372Sequence analysisAdd BLAST124
Coiled coili413 – 443Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438. Eukaryota.
ENOG410ZI4C. LUCA.
GeneTreeiENSGT00390000004199.
HOGENOMiHOG000290701.
HOVERGENiHBG082069.
InParanoidiQ6AYL9.
KOiK11548.
OMAiDCYAKID.
OrthoDBiEOG091G0YDU.
PhylomeDBiQ6AYL9.
TreeFamiTF101067.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AYL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLSFPRYN IAEIVVHIRN KLLTGADGKN LSKSDFLPNP KPEVLYMIYM
60 70 80 90 100
RALQLVYGVR LEHFYMMPVN IEVMYPHIME GFLPVSNLFF HLDSFMPICR
110 120 130 140 150
VNDFEIADIL YPKANRTSRF LSGIINFIHF RETCLEKYEE FLLQNKSSVD
160 170 180 190 200
KIQQLSNAHQ EALMKLEKLN SVPVEEQEEF KQLKDDIQEL QHLLNQDFRQ
210 220 230 240 250
KTTLLQERYT KMKSDFSEKT KHVNELKLSV VSLKEVQDSL KSKIVDSPEK
260 270 280 290 300
LKNYKEKMKD TVQKLRSARE EVMEKYDIYR DSVDCLPSCQ LEVQLYQKKS
310 320 330 340 350
QDLADNREKL SSILKESLNL EGQIDSDSSE LKKLKTEENS LIRLMTLKKE
360 370 380 390 400
RLATMQFKIN KKQEDVKQYK RTMIEDCNKV QEKRDAVCEQ VTAINQDIHK
410 420 430 440 450
IKSGIQQLRD AEKREKLKSQ EILVDLKSAL EKYHEGIEKT TEECCTRIGG
460
KTAELKRRMF KMPP
Length:464
Mass (Da):54,556
Last modified:September 13, 2004 - v1
Checksum:i5C0BFD08BB492DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078993 mRNA. Translation: AAH78993.1.
RefSeqiNP_001012028.1. NM_001012028.1.
UniGeneiRn.41428.

Genome annotation databases

EnsembliENSRNOT00000003650; ENSRNOP00000003650; ENSRNOG00000002711.
GeneIDi304951.
KEGGirno:304951.
UCSCiRGD:1307952. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078993 mRNA. Translation: AAH78993.1.
RefSeqiNP_001012028.1. NM_001012028.1.
UniGeneiRn.41428.

3D structure databases

ProteinModelPortaliQ6AYL9.
SMRiQ6AYL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003650.

Proteomic databases

PaxDbiQ6AYL9.
PRIDEiQ6AYL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003650; ENSRNOP00000003650; ENSRNOG00000002711.
GeneIDi304951.
KEGGirno:304951.
UCSCiRGD:1307952. rat.

Organism-specific databases

CTDi83540.
RGDi1307952. Nuf2.

Phylogenomic databases

eggNOGiKOG4438. Eukaryota.
ENOG410ZI4C. LUCA.
GeneTreeiENSGT00390000004199.
HOGENOMiHOG000290701.
HOVERGENiHBG082069.
InParanoidiQ6AYL9.
KOiK11548.
OMAiDCYAKID.
OrthoDBiEOG091G0YDU.
PhylomeDBiQ6AYL9.
TreeFamiTF101067.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ6AYL9.

Gene expression databases

BgeeiENSRNOG00000002711.
GenevisibleiQ6AYL9. RN.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUF2_RAT
AccessioniPrimary (citable) accession number: Q6AYL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.