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Protein

Kinetochore protein Nuf2

Gene

Nuf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2500257 Resolution of Sister Chromatid Cohesion
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:Nuf2
Synonyms:Cdca1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1307952 Nuf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498151 – 464Kinetochore protein Nuf2Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei171PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYL9
PRIDEiQ6AYL9

Expressioni

Gene expression databases

BgeeiENSRNOG00000002711
GenevisibleiQ6AYL9 RN

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003650

Structurei

3D structure databases

ProteinModelPortaliQ6AYL9
SMRiQ6AYL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80By similarityAdd BLAST385
Regioni386 – 464Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili148 – 201Sequence analysisAdd BLAST54
Coiled coili249 – 372Sequence analysisAdd BLAST124
Coiled coili413 – 443Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438 Eukaryota
ENOG410ZI4C LUCA
GeneTreeiENSGT00390000004199
HOGENOMiHOG000290701
HOVERGENiHBG082069
InParanoidiQ6AYL9
KOiK11548
OMAiREIVGCI
OrthoDBiEOG091G0YDU
PhylomeDBiQ6AYL9
TreeFamiTF101067

Family and domain databases

Gene3Di1.10.418.60, 1 hit
InterProiView protein in InterPro
IPR005549 Kinetochore_Nuf2
IPR038275 Nuf2_N_sf
PfamiView protein in Pfam
PF03800 Nuf2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AYL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLSFPRYN IAEIVVHIRN KLLTGADGKN LSKSDFLPNP KPEVLYMIYM
60 70 80 90 100
RALQLVYGVR LEHFYMMPVN IEVMYPHIME GFLPVSNLFF HLDSFMPICR
110 120 130 140 150
VNDFEIADIL YPKANRTSRF LSGIINFIHF RETCLEKYEE FLLQNKSSVD
160 170 180 190 200
KIQQLSNAHQ EALMKLEKLN SVPVEEQEEF KQLKDDIQEL QHLLNQDFRQ
210 220 230 240 250
KTTLLQERYT KMKSDFSEKT KHVNELKLSV VSLKEVQDSL KSKIVDSPEK
260 270 280 290 300
LKNYKEKMKD TVQKLRSARE EVMEKYDIYR DSVDCLPSCQ LEVQLYQKKS
310 320 330 340 350
QDLADNREKL SSILKESLNL EGQIDSDSSE LKKLKTEENS LIRLMTLKKE
360 370 380 390 400
RLATMQFKIN KKQEDVKQYK RTMIEDCNKV QEKRDAVCEQ VTAINQDIHK
410 420 430 440 450
IKSGIQQLRD AEKREKLKSQ EILVDLKSAL EKYHEGIEKT TEECCTRIGG
460
KTAELKRRMF KMPP
Length:464
Mass (Da):54,556
Last modified:September 13, 2004 - v1
Checksum:i5C0BFD08BB492DC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078993 mRNA Translation: AAH78993.1
RefSeqiNP_001012028.1, NM_001012028.1
UniGeneiRn.41428

Genome annotation databases

EnsembliENSRNOT00000003650; ENSRNOP00000003650; ENSRNOG00000002711
GeneIDi304951
KEGGirno:304951
UCSCiRGD:1307952 rat

Similar proteinsi

Entry informationi

Entry nameiNUF2_RAT
AccessioniPrimary (citable) accession number: Q6AYL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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