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Protein

Mitochondria-eating protein

Gene

Spata18

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). May have a role in spermatogenesis, especially in cell differentiation from late elongate spermatids to mature spermatozoa.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondria-eating protein
Alternative name(s):
Spermatid-expressing gene 1 protein
Short name:
Spetex-1
Spermatogenesis-associated protein 18
Gene namesi
Name:Spata18
Synonyms:Mieap, Spetex1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735208. Spata18.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Mitochondrion outer membrane By similarity

  • Note: Localizes to the cytoplasm under normal conditions. Relocalizes to mitochondrion outer membrane following cellular stress.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • intracellular membrane-bounded organelle Source: UniProtKB
  • mitochondrial outer membrane Source: UniProtKB
  • sperm flagellum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Mitochondria-eating proteinPRO_0000254167Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineCombined sources
Modified residuei85 – 851PhosphoserineCombined sources
Modified residuei123 – 1231PhosphoserineCombined sources
Modified residuei127 – 1271PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineCombined sources
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei303 – 3031PhosphoserineCombined sources
Modified residuei305 – 3051PhosphoserineCombined sources
Modified residuei527 – 5271PhosphoserineCombined sources
Isoform 2 (identifier: Q6AYL6-2)
Modified residuei541 – 5411PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AYL6.
PRIDEiQ6AYL6.

Expressioni

Tissue specificityi

High expressed in testis and weakly expressed in lung, intestine, and spleen. Not expressed in spermatogonia, spermatocytes, and round spematids in adult testis but specifically detected in elongated spermatids.1 Publication

Developmental stagei

Expressed at late stages of spermatogenesis, from late to maturing spermatids.1 Publication

Interactioni

Subunit structurei

Interacts (via coiled-coil domains) with BNIP3L (via BH3 domain). Interacts (via coiled-coil domains) with BNIP3 (via BH3 domain). Colocalizes with BNIP3 and BNIP3L at the mitochondrion outer membrane.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002899.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili152 – 18433Sequence analysisAdd
BLAST
Coiled coili210 – 24334Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi265 – 31349Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the MIEAP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGBW. Eukaryota.
ENOG410XRYY. LUCA.
HOVERGENiHBG093990.
InParanoidiQ6AYL6.
OMAiNTCDQNL.
OrthoDBiEOG7CG702.
PhylomeDBiQ6AYL6.
TreeFamiTF328808.

Family and domain databases

InterProiIPR026169. MIEAP.
IPR031981. MIEAP_C.
[Graphical view]
PANTHERiPTHR21771. PTHR21771. 2 hits.
PfamiPF16026. MIEAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6AYL6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAENLKKLAQ SESFLSLQDK VCFWANDYQT NSCDQNLNCC IELIEQVAKV
60 70 80 90 100
QAQLFAILTA TAQEGGHNEG VETIKSRLLP LLQTSFSSLG VGKIIQTEIR
110 120 130 140 150
PIQDFQLRSK NRQSSLDQEQ QQSDGDSFIE SQPTQVQDDL TESGKNLEGT
160 170 180 190 200
KNGSAISLLA AEEEINQLKK QLKSLQAQED ARHKTSENRR AEISRTDRRP
210 220 230 240 250
SGKRNSERPQ DVVSNYEKHL KNLKDEIAVL SAEKSGLQAS HRQTRSRSPS
260 270 280 290 300
PGPRGPRGPR GRRRSRSHSR SHSRSHSRSH SHSNSHSNSR SNSPCTTVAK
310 320 330 340 350
IRSPSPNRSK MSSVARKAAL LSRFSDAYSQ ARLDAQCLLR RCIDRAETVQ
360 370 380 390 400
RIIYIATVEA FHIAKMAFRH FKIRVRKMLT PSHVGSNDFE TAVSDYIVCH
410 420 430 440 450
LDLYDSQSSV NDVIRAMNVN PKISFPPEVD FCLLTDFIQE ICCIAFAMQS
460 470 480 490 500
LEPPLDIAFG ADGEVFNEAK YRRSYDSDFT APLVFYHVWP ALMENDCVIM
510 520 530 540 550
KGEAVTKRGA FWTTVRPVMR CRSRSLSPIC PRSRFGISTV HSCVTIPADD
560
LVSRSRSPSP IRCTFARY
Length:568
Mass (Da):63,884
Last modified:September 13, 2004 - v1
Checksum:i551981E551F59D73
GO
Isoform 2 (identifier: Q6AYL6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     541-552: Missing.

Show »
Length:556
Mass (Da):62,633
Checksum:iE1B8AD32ABD0D6F4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei541 – 55212Missing in isoform 2. 1 PublicationVSP_021189Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116526 mRNA. Translation: BAD02927.1.
BC078996 mRNA. Translation: AAH78996.1.
RefSeqiNP_955406.2. NM_199374.2. [Q6AYL6-1]
XP_006250953.1. XM_006250891.2. [Q6AYL6-2]
UniGeneiRn.27773.

Genome annotation databases

GeneIDi289586.
KEGGirno:289586.
UCSCiRGD:735208. rat. [Q6AYL6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116526 mRNA. Translation: BAD02927.1.
BC078996 mRNA. Translation: AAH78996.1.
RefSeqiNP_955406.2. NM_199374.2. [Q6AYL6-1]
XP_006250953.1. XM_006250891.2. [Q6AYL6-2]
UniGeneiRn.27773.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002899.

Proteomic databases

PaxDbiQ6AYL6.
PRIDEiQ6AYL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi289586.
KEGGirno:289586.
UCSCiRGD:735208. rat. [Q6AYL6-1]

Organism-specific databases

CTDi132671.
RGDi735208. Spata18.

Phylogenomic databases

eggNOGiENOG410IGBW. Eukaryota.
ENOG410XRYY. LUCA.
HOVERGENiHBG093990.
InParanoidiQ6AYL6.
OMAiNTCDQNL.
OrthoDBiEOG7CG702.
PhylomeDBiQ6AYL6.
TreeFamiTF328808.

Miscellaneous databases

NextBioi630020.
PROiQ6AYL6.

Family and domain databases

InterProiIPR026169. MIEAP.
IPR031981. MIEAP_C.
[Graphical view]
PANTHERiPTHR21771. PTHR21771. 2 hits.
PfamiPF16026. MIEAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complementary DNA cloning of rat spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids."
    Iida H., Ichinose J., Kaneko T., Mori T., Shibata Y.
    Mol. Reprod. Dev. 68:385-393(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-85; SER-123; SER-154; SER-157; SER-303; SER-305 AND SER-527, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMIEAP_RAT
AccessioniPrimary (citable) accession number: Q6AYL6
Secondary accession number(s): D3ZEU0, Q764P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.