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Protein

Splicing factor 3B subunit 4

Gene

Sf3b4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-RNO-72163 mRNA Splicing - Major Pathway
R-RNO-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 4
Gene namesi
Name:Sf3b4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi1309667 Sf3b4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003285862 – 424Splicing factor 3B subunit 4Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei56PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYL5
PRIDEiQ6AYL5

Expressioni

Gene expression databases

BgeeiENSRNOG00000021181

Interactioni

Subunit structurei

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with SF3B2. Found in a complex with PRMT9, SF3B2 and SF3B4. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028758

Structurei

3D structure databases

ProteinModelPortaliQ6AYL5
SMRiQ6AYL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 91RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini100 – 179RRM 2PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi215 – 218Poly-Pro4
Compositional biasi262 – 268Poly-Pro7

Sequence similaritiesi

Belongs to the SF3B4 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0131 Eukaryota
ENOG410XPT4 LUCA
GeneTreeiENSGT00870000136537
HOGENOMiHOG000200535
HOVERGENiHBG002295
InParanoidiQ6AYL5
KOiK12831
OMAiPNAWAPP
OrthoDBiEOG091G0HAI
PhylomeDBiQ6AYL5
TreeFamiTF300890

Family and domain databases

CDDicd12334 RRM1_SF3B4, 1 hit
cd12335 RRM2_SF3B4, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034158 SF3B4_RRM1
IPR034159 SF3B4_RRM2
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT
60 70 80 90 100
GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKAS AHNKNLDVGA
110 120 130 140 150
NIFIGNLDPE IDEKLLYDTF SAFGVILQTP KIMRDPDTGN SKGYAFINFA
160 170 180 190 200
SFDASDAAIE AMNGQYLCNR PITVSYAFKK DSKGERHGSA AERLLAAQNP
210 220 230 240 250
LSQADRPHQL FADAPPPPSA PNPVVSSLGS GLPPPGMPPP GSFPPPVPPP
260 270 280 290 300
GALPPGIPPA MPPPPMPPGA GGHGPPAAGT PGAGHPGHGH SHPHPFPPGG
310 320 330 340 350
MPHPGMSQMQ LAHHGPHGLG HPHAGPPGSG GQPPPRPPPG MPHPGPPPMG
360 370 380 390 400
MPPRGPPFGS PMGHPGPMPP HGMRGPPPLM PPHGYTGPPR PPPYGYQRGP
410 420
LPPPRPTPRP PVPPRGPLRG PLPQ
Length:424
Mass (Da):44,356
Last modified:September 13, 2004 - v1
Checksum:i7B6B69A2BD95F1E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078997 mRNA Translation: AAH78997.1
RefSeqiNP_001011951.1, NM_001011951.1
UniGeneiRn.116275

Genome annotation databases

EnsembliENSRNOT00000028758; ENSRNOP00000028758; ENSRNOG00000021181
GeneIDi295270
KEGGirno:295270
UCSCiRGD:1309667 rat

Similar proteinsi

Entry informationi

Entry nameiSF3B4_RAT
AccessioniPrimary (citable) accession number: Q6AYL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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