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Protein

Calcyclin-binding protein

Gene

Cacybp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) (By similarity).By similarity

GO - Biological processi

  • aging Source: RGD
  • cardiac muscle cell differentiation Source: RGD
  • cellular response to calcium ion Source: RGD
  • heart development Source: RGD
  • negative regulation of cell death Source: RGD
  • positive regulation of DNA replication Source: RGD
  • response to growth hormone Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Calcyclin-binding protein
Short name:
CacyBP
Gene namesi
Name:Cacybp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1303146. Cacybp.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: RGD
  • cytoplasm Source: RGD
  • neuron projection Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 229228Calcyclin-binding proteinPRO_0000271391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei21 – 211N6-acetyllysineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei86 – 861N6-acetyllysineBy similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYK6.
PRIDEiQ6AYK6.

PTM databases

iPTMnetiQ6AYK6.
PhosphoSiteiQ6AYK6.

Expressioni

Gene expression databases

ExpressionAtlasiQ6AYK6. baseline.
GenevisibleiQ6AYK6. RN.

Interactioni

Subunit structurei

Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1 (By similarity). Interacts with protein of the S100 family S100A1, S100A6, S100B, S100P and S100A12 in a calcium-dependent manner.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003586.

Structurei

3D structure databases

ProteinModelPortaliQ6AYK6.
SMRiQ6AYK6. Positions 1-55, 62-181, 189-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 16895CSPROSITE-ProRule annotationAdd
BLAST
Domaini169 – 22961SGSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 8180Interaction with SIAH1By similarityAdd
BLAST
Regioni74 – 229156Interaction with SKP1By similarityAdd
BLAST
Regioni155 – 22975Interaction with S100A6By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 CS domain.PROSITE-ProRule annotation
Contains 1 SGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ6AYK6.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG7K9K4J.
PhylomeDBiQ6AYK6.
TreeFamiTF323891.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASALEELQK DLEEVKVLLE KSTRKRLRDT LTNEKSKIET ELRNKMQQKS
60 70 80 90 100
QKKPEFDNEK PAAVVAPLTT GYTVKISNYG WDQSDKFVKI YITLTGVHQV
110 120 130 140 150
PAENVQVHFT ERSFDLLVKN LNGKNYSMIV NNLLKPISVE SSSKKVKTDT
160 170 180 190 200
VIILCRKKAE NTRWDYLTQV EKECKEKEKP SYDTEADPSE GLMNVLKKIY
210 220
EDGDDDMKRT INKAWVESRE KQAREDTEF
Length:229
Mass (Da):26,541
Last modified:September 13, 2004 - v1
Checksum:iE5158FA10314CC1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079007 mRNA. Translation: AAH79007.1.
RefSeqiNP_001004208.1. NM_001004208.1.
UniGeneiRn.11540.

Genome annotation databases

EnsembliENSRNOT00000003586; ENSRNOP00000003586; ENSRNOG00000002572.
ENSRNOT00000092356; ENSRNOP00000075861; ENSRNOG00000061959.
GeneIDi289144.
KEGGirno:289144.
UCSCiRGD:1303146. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079007 mRNA. Translation: AAH79007.1.
RefSeqiNP_001004208.1. NM_001004208.1.
UniGeneiRn.11540.

3D structure databases

ProteinModelPortaliQ6AYK6.
SMRiQ6AYK6. Positions 1-55, 62-181, 189-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003586.

PTM databases

iPTMnetiQ6AYK6.
PhosphoSiteiQ6AYK6.

Proteomic databases

PaxDbiQ6AYK6.
PRIDEiQ6AYK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003586; ENSRNOP00000003586; ENSRNOG00000002572.
ENSRNOT00000092356; ENSRNOP00000075861; ENSRNOG00000061959.
GeneIDi289144.
KEGGirno:289144.
UCSCiRGD:1303146. rat.

Organism-specific databases

CTDi27101.
RGDi1303146. Cacybp.

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ6AYK6.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG7K9K4J.
PhylomeDBiQ6AYK6.
TreeFamiTF323891.

Miscellaneous databases

PROiQ6AYK6.

Gene expression databases

ExpressionAtlasiQ6AYK6. baseline.
GenevisibleiQ6AYK6. RN.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Lubec G., Afjehi-Sadat L., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 38-43 AND 53-75, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Spinal cord.
  3. "CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family."
    Filipek A., Jastrzebska B., Nowotny M., Kuznicki J.
    J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH S100A1; S100A6; S100B; S100P AND S100A12.

Entry informationi

Entry nameiCYBP_RAT
AccessioniPrimary (citable) accession number: Q6AYK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: September 13, 2004
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.