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Protein

Non-homologous end-joining factor 1

Gene

Nhej1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.By similarity

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: UniProtKB
  • double-strand break repair via nonhomologous end joining Source: UniProtKB
  • response to ionizing radiation Source: UniProtKB
  • T cell differentiation Source: UniProtKB

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-RNO-5693571 Nonhomologous End-Joining (NHEJ)

Names & Taxonomyi

Protein namesi
Recommended name:
Non-homologous end-joining factor 1
Alternative name(s):
Protein cernunnos
XRCC4-like factor
Gene namesi
Name:Nhej1
Synonyms:Xlf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1359338 Nhej1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002286561 – 304Non-homologous end-joining factor 1Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AYI4

PTM databases

iPTMnetiQ6AYI4
PhosphoSitePlusiQ6AYI4

Expressioni

Gene expression databases

BgeeiENSRNOG00000018162
GenevisibleiQ6AYI4 RN

Interactioni

Subunit structurei

Exists mainly as a homodimer. Associates with the non-homologous end joining (NHEJ) complex which is at least composed of the core proteins XRCC5/Ku80, XRCC6/Ku70, PRKDC/DNA-PKcs, LIG4 and XRCC4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024444

Structurei

3D structure databases

ProteinModelPortaliQ6AYI4
SMRiQ6AYI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 140Globular headBy similarityAdd BLAST140

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili133 – 175By similarityAdd BLAST43

Domaini

The coiled-coil region mediates homodimerization.By similarity

Sequence similaritiesi

Belongs to the XLF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IT7Y Eukaryota
ENOG411291V LUCA
GeneTreeiENSGT00390000009940
HOGENOMiHOG000089974
HOVERGENiHBG080703
InParanoidiQ6AYI4
KOiK10980
OMAiYWNFHCI
OrthoDBiEOG091G1269
PhylomeDBiQ6AYI4
TreeFamiTF328567

Family and domain databases

InterProiView protein in InterPro
IPR015381 XLF_fam
PfamiView protein in Pfam
PF09302 XLF, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AYI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELEQGLLM QPWAWLQLAE NSLLAKASIT KHGYALLISD LQQVWHEQVD
60 70 80 90 100
TLEVSQRAKE LNKRLTAPPA AFLHHLDEVL RPLFKDSAHQ DAAHPSKATF
110 120 130 140 150
SCDRGEEVLI LRVRSELSGL PFNWHFHCLP ASSLLVSQHL ICPLMGVSLA
160 170 180 190 200
LHSHVRELAA LLRMKDLEIQ AYQESGAVLS RGRLKTEPFE ENSFLEQFMV
210 220 230 240 250
EKLPEACAVG DGRPFAMNLQ SLYVAVTKQQ VQARQKHKGS GEPQTSSSTS
260 270 280 290 300
PQGTDSQLQN QPEQQISPTP TLSEPECEPM AASGPVHRAQ LVKAKRKKPR

GLFS
Length:304
Mass (Da):33,885
Last modified:September 13, 2004 - v1
Checksum:iDDB56676EB94B4DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079033 mRNA Translation: AAH79033.1
RefSeqiNP_001014239.1, NM_001014217.1
XP_006245302.1, XM_006245240.1
XP_006245303.1, XM_006245241.3
XP_006245304.1, XM_006245242.3
UniGeneiRn.35483

Genome annotation databases

EnsembliENSRNOT00000024444; ENSRNOP00000024444; ENSRNOG00000018162
GeneIDi363251
KEGGirno:363251
UCSCiRGD:1359338 rat

Similar proteinsi

Entry informationi

Entry nameiNHEJ1_RAT
AccessioniPrimary (citable) accession number: Q6AYI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: September 13, 2004
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health