Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-homologous end-joining factor 1

Gene

Nhej1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: UniProtKB
  • DNA recombination Source: InterPro
  • double-strand break repair via nonhomologous end joining Source: UniProtKB
  • response to ionizing radiation Source: UniProtKB
  • T cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-homologous end-joining factor 1
Alternative name(s):
Protein cernunnos
XRCC4-like factor
Gene namesi
Name:Nhej1
Synonyms:Xlf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1359338. Nhej1.

Subcellular locationi

GO - Cellular componenti

  • nonhomologous end joining complex Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Non-homologous end-joining factor 1PRO_0000228656Add
BLAST

Proteomic databases

PaxDbiQ6AYI4.

PTM databases

PhosphoSiteiQ6AYI4.

Expressioni

Gene expression databases

GenevisibleiQ6AYI4. RN.

Interactioni

Subunit structurei

Exists mainly as a homodimer. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024444.

Structurei

3D structure databases

ProteinModelPortaliQ6AYI4.
SMRiQ6AYI4. Positions 1-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 140140Globular headBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili133 – 17543By similarityAdd
BLAST

Domaini

The coiled-coil region mediates homodimerization.By similarity

Sequence similaritiesi

Belongs to the XLF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG47408.
GeneTreeiENSGT00390000009940.
HOGENOMiHOG000089974.
HOVERGENiHBG080703.
InParanoidiQ6AYI4.
KOiK10980.
OMAiQSLYMAV.
OrthoDBiEOG7Z0JXT.
PhylomeDBiQ6AYI4.
TreeFamiTF328567.

Family and domain databases

Gene3Di2.170.210.10. 1 hit.
InterProiIPR015381. XLF_fam.
IPR009089. XRCC4_N.
[Graphical view]
PfamiPF09302. XLF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AYI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELEQGLLM QPWAWLQLAE NSLLAKASIT KHGYALLISD LQQVWHEQVD
60 70 80 90 100
TLEVSQRAKE LNKRLTAPPA AFLHHLDEVL RPLFKDSAHQ DAAHPSKATF
110 120 130 140 150
SCDRGEEVLI LRVRSELSGL PFNWHFHCLP ASSLLVSQHL ICPLMGVSLA
160 170 180 190 200
LHSHVRELAA LLRMKDLEIQ AYQESGAVLS RGRLKTEPFE ENSFLEQFMV
210 220 230 240 250
EKLPEACAVG DGRPFAMNLQ SLYVAVTKQQ VQARQKHKGS GEPQTSSSTS
260 270 280 290 300
PQGTDSQLQN QPEQQISPTP TLSEPECEPM AASGPVHRAQ LVKAKRKKPR

GLFS
Length:304
Mass (Da):33,885
Last modified:September 13, 2004 - v1
Checksum:iDDB56676EB94B4DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079033 mRNA. Translation: AAH79033.1.
RefSeqiNP_001014239.1. NM_001014217.1.
XP_006245302.1. XM_006245240.1.
XP_006245303.1. XM_006245241.2.
XP_006245304.1. XM_006245242.2.
UniGeneiRn.35483.

Genome annotation databases

EnsembliENSRNOT00000024444; ENSRNOP00000024444; ENSRNOG00000018162.
GeneIDi363251.
KEGGirno:363251.
UCSCiRGD:1359338. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079033 mRNA. Translation: AAH79033.1.
RefSeqiNP_001014239.1. NM_001014217.1.
XP_006245302.1. XM_006245240.1.
XP_006245303.1. XM_006245241.2.
XP_006245304.1. XM_006245242.2.
UniGeneiRn.35483.

3D structure databases

ProteinModelPortaliQ6AYI4.
SMRiQ6AYI4. Positions 1-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024444.

PTM databases

PhosphoSiteiQ6AYI4.

Proteomic databases

PaxDbiQ6AYI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024444; ENSRNOP00000024444; ENSRNOG00000018162.
GeneIDi363251.
KEGGirno:363251.
UCSCiRGD:1359338. rat.

Organism-specific databases

CTDi79840.
RGDi1359338. Nhej1.

Phylogenomic databases

eggNOGiNOG47408.
GeneTreeiENSGT00390000009940.
HOGENOMiHOG000089974.
HOVERGENiHBG080703.
InParanoidiQ6AYI4.
KOiK10980.
OMAiQSLYMAV.
OrthoDBiEOG7Z0JXT.
PhylomeDBiQ6AYI4.
TreeFamiTF328567.

Miscellaneous databases

NextBioi683004.
PROiQ6AYI4.

Gene expression databases

GenevisibleiQ6AYI4. RN.

Family and domain databases

Gene3Di2.170.210.10. 1 hit.
InterProiIPR015381. XLF_fam.
IPR009089. XRCC4_N.
[Graphical view]
PfamiPF09302. XLF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiNHEJ1_RAT
AccessioniPrimary (citable) accession number: Q6AYI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: September 13, 2004
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.