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Protein

E3 ubiquitin-protein ligase RNF34

Gene

Rnf34

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 107FYVE-typeAdd BLAST52
Zinc fingeri334 – 369RING-typePROSITE-ProRule annotationAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-6804757. Regulation of TP53 Degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF34Curated (EC:2.3.2.271 Publication)
Alternative name(s):
RING finger protein 34Imported
RING finger protein MOMO1 Publication
RING-type E3 ubiquitin transferase RNF34Curated
Gene namesi
Name:Rnf34Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi628636. Rnf34.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560751 – 381E3 ubiquitin-protein ligase RNF34Add BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineBy similarity1
Modified residuei263PhosphoserineBy similarity1
Modified residuei265PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved by caspases upon induction of apoptosis by TNF.By similarity
Autoubiquitinated (in vitro).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei241 – 242Cleavage; by caspase-3By similarity2

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AYH3.

Expressioni

Tissue specificityi

Ubiquitous. Detected in brain, cerebellum, midbrain, hippocampus, striatum, heart, lung, kidney, muscle, spleen and testis.1 Publication

Gene expression databases

BgeeiENSRNOG00000001331.
GenevisibleiQ6AYH3. RN.

Interactioni

Subunit structurei

Interacts with CASP8 and CASP10. Interacts with p53/TP53; involved in p53/TP53 ubiquitination. Interacts (via RING-type zinc finger) with MDM2; the interaction stabilizes MDM2. Interacts (via RING-type zinc finger) with PPARGC1A. Interacts with NOD1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001799.

Structurei

3D structure databases

ProteinModelPortaliQ6AYH3.
SMRiQ6AYH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 134SAP 1Add BLAST20
Domaini273 – 287SAP 2Add BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi222 – 250Asp/Glu-richAdd BLAST29

Domaini

The RING-type zinc finger is required for the ubiquitination of target proteins.By similarity
The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 107FYVE-typeAdd BLAST52
Zinc fingeri334 – 369RING-typePROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IMEF. Eukaryota.
ENOG410Y1GP. LUCA.
GeneTreeiENSGT00390000012719.
HOGENOMiHOG000068080.
HOVERGENiHBG055079.
InParanoidiQ6AYH3.
KOiK20804.
OMAiMGGDRTL.
OrthoDBiEOG091G0S1S.
PhylomeDBiQ6AYH3.
TreeFamiTF325195.

Family and domain databases

Gene3Di1.10.720.30. 2 hits.
3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR003034. SAP_dom.
IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SUPFAMiSSF57903. SSF57903. 1 hit.
SSF68906. SSF68906. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AYH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAGATSMWA SCCGLLNEVM GTGAVRGQQA GFPGSTGPFR FTPSSDFPTY
60 70 80 90 100
PPAATEGPNI VCKACGLSFS VFRKKHVCCD CKKDFCSLCS VSQENLRRCS
110 120 130 140 150
TCHLLQETAF QRPQLMRLKV KDLRQYLLLR NVPTDTCREK EDLVDLVLCH
160 170 180 190 200
RGLGSGDGLD SRSLSSSRSQ TSSFFTQSYF SNYTPPSATV SSFQGELMDR
210 220 230 240 250
EGTFRSEVLT QVQSELASAN TDDEDGEEDD DDDDDDDDED DDEQEENLEE
260 270 280 290 300
QNPGLSKKKA RASLSDLSSL EEVEGMSVRQ LKEILARNFV NYSGCCEKWE
310 320 330 340 350
LVEKVNRLYK ENEENQKSYG ERMQLQDEED DSLCRICMDA VIDCVLLECG
360 370 380
HMVTCTKCGK RMSECPICRQ YVVRAVHVFK S
Length:381
Mass (Da):42,681
Last modified:September 13, 2004 - v1
Checksum:i12A2E7BEB1CEA4EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253P → L in AAN60073 (PubMed:12859687).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157968 mRNA. Translation: AAN60073.1.
BC079044 mRNA. Translation: AAH79044.1.
RefSeqiNP_001004075.1. NM_001004075.1.
UniGeneiRn.14904.

Genome annotation databases

EnsembliENSRNOT00000001799; ENSRNOP00000001799; ENSRNOG00000001331.
GeneIDi282845.
KEGGirno:282845.
UCSCiRGD:628636. rat.

Similar proteinsi

Entry informationi

Entry nameiRNF34_RAT
AccessioniPrimary (citable) accession number: Q6AYH3
Secondary accession number(s): Q8CIP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 13, 2004
Last modified: August 30, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways