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Protein

Target of rapamycin complex 2 subunit MAPKAP1

Gene

Mapkap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-421'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

ReactomeiR-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-389357. CD28 dependent PI3K/Akt signaling.
R-RNO-5218920. VEGFR2 mediated vascular permeability.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit MAPKAP1
Short name:
TORC2 subunit MAPKAP1
Alternative name(s):
Mitogen-activated protein kinase 2-associated protein 1
Stress-activated map kinase-interacting protein 1
Short name:
SAPK-interacting protein 1
Gene namesi
Name:Mapkap1
Synonyms:Mip1, Sin1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1305363. Mapkap1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 522521Target of rapamycin complex 2 subunit MAPKAP1PRO_0000328036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei510 – 5101PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYF1.
PRIDEiQ6AYF1.

PTM databases

PhosphoSiteiQ6AYF1.

Expressioni

Gene expression databases

GenevisibleiQ6AYF1. RN.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2, NBN and SGK1. Interacts with CCDC28B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023889.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 267266Interaction with NBNBy similarityAdd
BLAST
Regioni2 – 184183Interaction with MAP3K2By similarityAdd
BLAST
Regioni468 – 52255Interaction with ATF2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SIN1 family.Curated

Phylogenomic databases

eggNOGiKOG3739. Eukaryota.
ENOG410XR57. LUCA.
GeneTreeiENSGT00390000000642.
HOGENOMiHOG000044319.
HOVERGENiHBG023148.
InParanoidiQ6AYF1.
OMAiLCACDLV.
OrthoDBiEOG7GXPBF.
PhylomeDBiQ6AYF1.
TreeFamiTF315174.

Family and domain databases

InterProiIPR031567. CRIM_dom.
IPR008828. Sin1/Avo1.
IPR032679. Sin1_N.
IPR031313. Sin1_PH_dom.
[Graphical view]
PANTHERiPTHR13335. PTHR13335. 1 hit.
PfamiPF16978. CRIM. 1 hit.
PF05422. SIN1. 1 hit.
PF16979. SIN1_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK THPPSVPGDS
60 70 80 90 100
GSEVQGSSGE TQGYIYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ
110 120 130 140 150
IKCKNIQWKE RNSKQSAQEL KSLFEKKSLK EKPPSSGKQS ILSVRLEQCP
160 170 180 190 200
LQLNNPFNEY SKFDGKGHVG TTATKKIDVY LPLHSSQDRL LPMTVVTMAS
210 220 230 240 250
ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE VDTDFPPLDS
260 270 280 290 300
NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT
310 320 330 340 350
MKEILLKALK RRKGSQKISG PQYRLEKQSE PNIGVDLEST LESQNAWEFC
360 370 380 390 400
LVRENSSRAD GVFEEDSQID IATVQDMLSS HHYKSFKVSM IHRLRFTTDV
410 420 430 440 450
QLGISGDKVE IDPVTNQKAS TKFWIKQKPI SIDCDLLCAC DLAEEKSPSH
460 470 480 490 500
AVFKLTYLSS HDYKHLYFES DAATVSEIVL KVNYILESRA STARADYFAQ
510 520
KQRKLNRRTS FSFQKEKKSG QQ
Length:522
Mass (Da):59,043
Last modified:September 13, 2004 - v1
Checksum:i7F96C228E60A8B88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079073 mRNA. Translation: AAH79073.1.
RefSeqiNP_001011964.1. NM_001011964.1.
XP_006234011.1. XM_006233949.2.
XP_006234012.1. XM_006233950.2.
UniGeneiRn.105886.

Genome annotation databases

EnsembliENSRNOT00000023889; ENSRNOP00000023889; ENSRNOG00000017583.
GeneIDi296648.
KEGGirno:296648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079073 mRNA. Translation: AAH79073.1.
RefSeqiNP_001011964.1. NM_001011964.1.
XP_006234011.1. XM_006233949.2.
XP_006234012.1. XM_006233950.2.
UniGeneiRn.105886.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023889.

PTM databases

PhosphoSiteiQ6AYF1.

Proteomic databases

PaxDbiQ6AYF1.
PRIDEiQ6AYF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023889; ENSRNOP00000023889; ENSRNOG00000017583.
GeneIDi296648.
KEGGirno:296648.

Organism-specific databases

CTDi79109.
RGDi1305363. Mapkap1.

Phylogenomic databases

eggNOGiKOG3739. Eukaryota.
ENOG410XR57. LUCA.
GeneTreeiENSGT00390000000642.
HOGENOMiHOG000044319.
HOVERGENiHBG023148.
InParanoidiQ6AYF1.
OMAiLCACDLV.
OrthoDBiEOG7GXPBF.
PhylomeDBiQ6AYF1.
TreeFamiTF315174.

Enzyme and pathway databases

ReactomeiR-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-389357. CD28 dependent PI3K/Akt signaling.
R-RNO-5218920. VEGFR2 mediated vascular permeability.

Miscellaneous databases

NextBioi641579.
PROiQ6AYF1.

Gene expression databases

GenevisibleiQ6AYF1. RN.

Family and domain databases

InterProiIPR031567. CRIM_dom.
IPR008828. Sin1/Avo1.
IPR032679. Sin1_N.
IPR031313. Sin1_PH_dom.
[Graphical view]
PANTHERiPTHR13335. PTHR13335. 1 hit.
PfamiPF16978. CRIM. 1 hit.
PF05422. SIN1. 1 hit.
PF16979. SIN1_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiSIN1_RAT
AccessioniPrimary (citable) accession number: Q6AYF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: March 16, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.