Q6AYE2 (SHLB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endophilin-B1

Alternative name(s):
SH3 domain-containing GRB2-like protein B1

Gene names

Name:

Sh3glb1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion By similarity. UniProtKB Q9Y371
Subunit structure	Homodimer, and heterodimer with SH3GLB2. Binds BAX. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 By similarity. UniProtKB Q9JK48 UniProtKB Q9Y371
Subcellular location	Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Note: Association with the Golgi apparatus depends on the cell type By similarity. Ref.2 UniProtKB Q9JK48 UniProtKB Q9Y371
Tissue specificity	Expressed in brain, heart, lung and spleen. Low level in liver and testis. Ref.2
Domain	An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. Ref.2
Post-translational modification	Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization By similarity.
Sequence similarities	Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Domain	Coiled coil SH3 domain
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW filopodium assembly Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: InterPro cytoskeletal adaptor activity Inferred from electronic annotation. Source: InterPro lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Endophilin-B1		PRO_0000307711
Regions
	Domain	27 – 261	235	BAR
	Domain	305 – 365	61	SH3
	Region	1 – 30	30	Membrane-binding amphipathic helix By similarity UniProtKB Q9Y371
	Coiled coil	156 – 185	30	Potential
Amino acid modifications
	Modified residue	145	1	Phosphothreonine; by CDK5 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AYE2 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: AF6950A4820FB470	FASTA	365	40,787
	10 20 30 40 50 60 MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE CTKVWTEKIM 70 80 90 100 110 120 KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY MIDAGTEFGP GTAYGNALIK 130 140 150 160 170 180 CGETQKRIGT ADRELIQTSA LNFLTPLRNF IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK 190 200 210 220 230 240 KAKAAETKSS SEQELRITQS EFDRQAEITR LLLEGISSTH AHHLRCLNDF VEAQMTYYAQ 250 260 270 280 290 300 CYQYMLDLQK QLGSFPSNYV SNNNQTSGTP VPYTLSNTIG PSAVASTGSL VITCPPNLSD 310 320 330 340 350 360 LKDSSSTRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[2]	"Generation of high curvature membranes mediated by direct endophilin bilayer interactions." Farsad K., Ringstad N., Takei K., Floyd S.R., Rose K., De Camilli P. J. Cell Biol. 155:193-200(2001) [PubMed: 11604418] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079085 mRNA. Translation: AAH79085.1.
IPI	IPI00392306.
RefSeq	NP_001011929.1. NM_001011929.1.
UniGene	Rn.203013.
3D structure databases
HSSP	HSSP built from PDB template 1X43 based on UniProtKB Q9JK48.
ProteinModelPortal	Q6AYE2.
SMR	Q6AYE2. Positions 298-365.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AYE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017770; ENSRNOP00000017771; ENSRNOG00000012957.
GeneID	292156.
KEGG	rno:292156.
UCSC	NM_001011929. rat.
Organism-specific databases
CTD	51100.
RGD	1304859. Sh3glb1.
Phylogenomic databases
eggNOG	roNOG10149.
GeneTree	ENSGT00550000074464.
HOVERGEN	HBG054448.
Gene expression databases
ArrayExpress	Q6AYE2.
Genevestigator	Q6AYE2.
Family and domain databases
InterPro	IPR004148. BAR_dom. IPR013606. IRSp53/MIM_homology_IMD. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:1.20.1270.80. IRSp53/MIM_homology_IMD. 1 hit.
KO	K11248.
Pfam	PF03114. BAR. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00721. BAR. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS51021. BAR. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	633869.

Entry information

Entry name

SHLB1_RAT

Accession

Primary (citable) accession number: Q6AYE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 13, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program