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Protein

Proliferation-associated protein 2G4

Gene

Pa2g4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Together with PTBP1 is required for the translation initiation on the foot-and-mouth disease virus (FMDV) IRES (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation.1 PublicationBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, RNA-binding
Biological processrRNA processing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-RNO-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiM24.978

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferation-associated protein 2G4
Gene namesi
Name:Pa2g4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1302994 Pa2g4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004315302 – 394Proliferation-associated protein 2G4Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei335PhosphoserineBy similarity1
Modified residuei361Phosphoserine; by PKC/PRKCDBy similarity1
Modified residuei366PhosphothreonineBy similarity1
Modified residuei386PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.By similarity
Isoform 2 is polyubiquitinated, leading to proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AYD3
PRIDEiQ6AYD3

PTM databases

iPTMnetiQ6AYD3
PhosphoSitePlusiQ6AYD3

Expressioni

Gene expression databases

BgeeiENSRNOG00000004904
ExpressionAtlasiQ6AYD3 baseline and differential
GenevisibleiQ6AYD3 RN

Interactioni

Subunit structurei

Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with nucleolin/NCL. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation (By similarity). Isoform 1 and isoform 2 interact with RNF20 (By similarity). Isoform 2 interacts with HUWE1 (By similarity). Interacts with AKT1. Interacts with DNAJC21 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6AYD3, 1 interactor
STRINGi10116.ENSRNOP00000006578

Structurei

3D structure databases

ProteinModelPortaliQ6AYD3
SMRiQ6AYD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 48Necessary for nucleolar localizationBy similarityAdd BLAST47
Regioni46 – 54RNA-bindingBy similarity9
Regioni301 – 394Necessary for nucleolar localizationBy similarityAdd BLAST94
Regioni361 – 375Interaction with RNABy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the peptidase M24 family.Curated

Phylogenomic databases

eggNOGiKOG2776 Eukaryota
COG0024 LUCA
GeneTreeiENSGT00530000063220
HOGENOMiHOG000168207
HOVERGENiHBG053117
OMAiHTVLLMP
OrthoDBiEOG091G0B89
PhylomeDBiQ6AYD3
TreeFamiTF300010

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR004545 PA2G4
IPR000994 Pept_M24
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00495 crvDNA_42K, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q6AYD3-1) [UniParc]FASTAAdd to basket
Also known as: p481 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE
60 70 80 90 100
KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL
110 120 130 140 150
KEGDLVKIDL GVHVDGFIAN VAHTFVIGVA QGSQVTGRKA DVIKAAHLCA
160 170 180 190 200
EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT
210 220 230 240 250
IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD
260 270 280 290 300
PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE
310 320 330 340 350
LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ
360 370 380 390
DAELKALLQS SASRKTQKKK KKKASKTAEN ATSGETLEEN GAGD
Length:394
Mass (Da):43,657
Last modified:September 13, 2004 - v1
Checksum:iB3799DCF064371BB
GO
Isoform 2Curated (identifier: Q6AYD3-2) [UniParc]FASTAAdd to basket
Also known as: p421 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:340
Mass (Da):37,929
Checksum:i1DA65807D48C815F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0573241 – 54Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06047173 Genomic DNA No translation available.
CH474104 Genomic DNA Translation: EDL84836.1
BC079095 mRNA Translation: AAH79095.1
RefSeqiNP_001004206.1, NM_001004206.1 [Q6AYD3-1]
UniGeneiRn.9485

Genome annotation databases

EnsembliENSRNOT00000006578; ENSRNOP00000006578; ENSRNOG00000004904 [Q6AYD3-1]
GeneIDi288778
KEGGirno:288778
UCSCiRGD:1302994 rat [Q6AYD3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPA2G4_RAT
AccessioniPrimary (citable) accession number: Q6AYD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: September 13, 2004
Last modified: February 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health