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Protein

CST complex subunit STN1

Gene

Obfc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation. However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of ot its role in telomere duplex replication (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi64 – 165102OBAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CST complex subunit STN1
Alternative name(s):
Oligonucleotide/oligosaccharide-binding fold-containing protein 1
Suppressor of cdc thirteen homolog
Gene namesi
Name:Obfc1
Synonyms:Stn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305637. Obfc1.

Subcellular locationi

  • Nucleus By similarity
  • Chromosometelomere By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 408407CST complex subunit STN1PRO_0000058022Add
BLAST

Proteomic databases

PaxDbiQ6AYD2.
PRIDEiQ6AYD2.

Expressioni

Gene expression databases

GenevisibleiQ6AYD2. RN.

Interactioni

Subunit structurei

Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1/OBFC1; in the complex interacts directly with TEN1 and CTC1. Interacts with ACD/TPP1, POT1 and POLA1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027614.

Structurei

3D structure databases

ProteinModelPortaliQ6AYD2.
SMRiQ6AYD2. Positions 205-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 195188Interaction with CTC1By similarityAdd
BLAST
Regioni201 – 304104Winged helix-turn-helix (wHTH) 1By similarityAdd
BLAST
Regioni305 – 408104Winged helix-turn-helix (wHTH) 1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the STN1 family.Curated
Contains 1 OB DNA-binding domain.Curated

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
HOVERGENiHBG053364.
InParanoidiQ6AYD2.
OMAiQTFYQQE.
OrthoDBiEOG76QFHT.
PhylomeDBiQ6AYD2.
TreeFamiTF328623.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 2 hits.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPCLLMQC ESSPKEEEIP SLFWGLDPVF LAFAKLYIKD ILEMKESQQV
60 70 80 90 100
PGMYFYNGHP IRRVDIMGAV ISVKERETFY SYGVDDATGV INCVCWKRPS
110 120 130 140 150
NAESSSDPAI LSTSRELSMT SQLKKLQETI EQKTKIGIGD IIRVRGYVRM
160 170 180 190 200
FREEREICAT IYYKVDDPVW NMQIARMLEL PELYKKVYDQ PFRNPALKEE
210 220 230 240 250
EALNSKDTLD LAGLTALLSE KVKEFLQEKK VQSFYQKELE MVEPLQSLAS
260 270 280 290 300
QPVTHSTCSD QVELKNDAAS DIHSVFKNAL HLLQEKGFVF QRDGGSDKLY
310 320 330 340 350
YVTSKDKDLH QKIYQIIKED CQKPNLWCML PQEAWRGTEE GLAVVVTLSV
360 370 380 390 400
CLPLPVDVEK GCHLMHVLNC VLLNLRWDLN KAVLQQVLEL LEDQSDIVST

GDHYYTAF
Length:408
Mass (Da):46,783
Last modified:September 13, 2004 - v1
Checksum:i068A9917A691ADA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079096 mRNA. Translation: AAH79096.1.
RefSeqiNP_001011943.1. NM_001011943.1.
UniGeneiRn.15899.

Genome annotation databases

GeneIDi294025.
KEGGirno:294025.
UCSCiRGD:1305637. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079096 mRNA. Translation: AAH79096.1.
RefSeqiNP_001011943.1. NM_001011943.1.
UniGeneiRn.15899.

3D structure databases

ProteinModelPortaliQ6AYD2.
SMRiQ6AYD2. Positions 205-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027614.

Proteomic databases

PaxDbiQ6AYD2.
PRIDEiQ6AYD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi294025.
KEGGirno:294025.
UCSCiRGD:1305637. rat.

Organism-specific databases

CTDi79991.
RGDi1305637. Obfc1.

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
HOVERGENiHBG053364.
InParanoidiQ6AYD2.
OMAiQTFYQQE.
OrthoDBiEOG76QFHT.
PhylomeDBiQ6AYD2.
TreeFamiTF328623.

Miscellaneous databases

PROiQ6AYD2.

Gene expression databases

GenevisibleiQ6AYD2. RN.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 2 hits.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiSTN1_RAT
AccessioniPrimary (citable) accession number: Q6AYD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: September 13, 2004
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.