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Protein

H/ACA ribonucleoprotein complex subunit 1

Gene

Gar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • snoRNA guided rRNA pseudouridine synthesis Source: RGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
Nucleolar protein family A member 1
snoRNP protein GAR1
Gene namesi
Name:Gar1
Synonyms:Nola1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1563995. Gar1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085541 – 226H/ACA ribonucleoprotein complex subunit 1Add BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ6AYA1.
PRIDEiQ6AYA1.

PTM databases

iPTMnetiQ6AYA1.
PhosphoSitePlusiQ6AYA1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000061146.
GenevisibleiQ6AYA1. RN.

Interactioni

Subunit structurei

Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) (By similarity).By similarity

Protein-protein interaction databases

CORUMiQ6AYA1.
STRINGi10116.ENSRNOP00000013149.

Structurei

3D structure databases

ProteinModelPortaliQ6AYA1.
SMRiQ6AYA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 59RGG-box 1Add BLAST56
Regioni171 – 226RGG-box 2Add BLAST56

Domaini

Interaction with SMN1 requires at least one of the RGG-box regions.By similarity

Sequence similaritiesi

Belongs to the GAR1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3262. Eukaryota.
COG3277. LUCA.
GeneTreeiENSGT00730000111223.
InParanoidiQ6AYA1.
KOiK11128.
OMAiFQQRDMG.
OrthoDBiEOG091G0Z6Z.
TreeFamiTF350747.

Family and domain databases

InterProiView protein in InterPro
IPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
PANTHERiPTHR23237. PTHR23237. 1 hit.
PfamiView protein in Pfam
PF04410. Gar1. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD020235. Gar1_RNA_bd. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AYA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRGGGRGG FNRGGGGGGF NRGGGSNNHF RGGGGGGGGG GNFRGGGRGG
60 70 80 90 100
FGRGGGRGGF NKFQDQGPPE RVVLLGEFMH PCEDDIVCKC TTEENKVPYF
110 120 130 140 150
NAPVYLENKE QIGKVDEIFG QLRDFYFSVK LSENMKASSF KKLQKFYIDP
160 170 180 190 200
YKLLPLQRFL PRPPGEKGPP RGGGGGGGGG RGGRGGGRGG GGRGGGRGGG
210 220
FRGGRGGGGG FRGGRGGGGG FRGRGH
Length:226
Mass (Da):23,011
Last modified:September 13, 2004 - v1
Checksum:iB81ADE63E02F151D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079131 mRNA. Translation: AAH79131.1.
RefSeqiNP_001019477.1. NM_001024306.1.
UniGeneiRn.15522.

Genome annotation databases

EnsembliENSRNOT00000078074; ENSRNOP00000074835; ENSRNOG00000061146.
GeneIDi499709.
KEGGirno:499709.
UCSCiRGD:1563995. rat.

Similar proteinsi

Entry informationi

Entry nameiGAR1_RAT
AccessioniPrimary (citable) accession number: Q6AYA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: September 27, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families