Q6AYA1 (GAR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H/ACA ribonucleoprotein complex subunit 1

Alternative name(s):
Nucleolar protein family A member 1
snoRNP protein GAR1

Gene names

Name:	Gar1
Synonyms:	Nola1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme By similarity.
Subunit structure	Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) By similarity.
Subcellular location	Nucleus › nucleolus By similarity. Nucleus › Cajal body By similarity. Note: Also localized to Cajal bodies (coiled bodies) By similarity.
Domain	Interaction with SMN1 requires at least one of the RGG-box regions By similarity.
Sequence similarities	Belongs to the GAR1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW snRNA pseudouridine synthesis Inferred from electronic annotation. Source: InterPro
Cellular_component	Cajal body Inferred from electronic annotation. Source: UniProtKB-SubCell box H/ACA snoRNP complex Inferred from electronic annotation. Source: InterPro
Molecular_function	pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro snoRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	H/ACA ribonucleoprotein complex subunit 1		PRO_0000208554
Regions
	Region	4 – 59	56	RGG-box 1
	Region	171 – 226	56	RGG-box 2

Sequences

	Sequence		Length	Mass (Da)
	Q6AYA1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: B81ADE63E02F151D	FASTA	226	23,011
	10 20 30 40 50 60 MSFRGGGRGG FNRGGGGGGF NRGGGSNNHF RGGGGGGGGG GNFRGGGRGG FGRGGGRGGF 70 80 90 100 110 120 NKFQDQGPPE RVVLLGEFMH PCEDDIVCKC TTEENKVPYF NAPVYLENKE QIGKVDEIFG 130 140 150 160 170 180 QLRDFYFSVK LSENMKASSF KKLQKFYIDP YKLLPLQRFL PRPPGEKGPP RGGGGGGGGG 190 200 210 220 RGGRGGGRGG GGRGGGRGGG FRGGRGGGGG FRGGRGGGGG FRGRGH

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079131 mRNA. Translation: AAH79131.1.
IPI	IPI00464655.
RefSeq	NP_001019477.1. NM_001024306.1.
UniGene	Rn.15522.
3D structure databases
ProteinModelPortal	Q6AYA1.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000013149.
Proteomic databases
PaxDb	Q6AYA1.
PRIDE	Q6AYA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013149; ENSRNOP00000013149; ENSRNOG00000009877.
GeneID	499709.
KEGG	rno:499709.
UCSC	RGD:1563995. rat.
Organism-specific databases
CTD	54433.
RGD	1563995. Gar1.
Phylogenomic databases
eggNOG	COG3277.
GeneTree	ENSGT00530000063105.
InParanoid	Q6AYA1.
KO	K11128.
OMA	ELYIDPG.
OrthoDB	EOG480HZ6.
Gene expression databases
ArrayExpress	Q6AYA1.
Genevestigator	Q6AYA1.
GermOnline	ENSRNOG00000009877. Rattus norvegicus.
Family and domain databases
InterPro	IPR007504. H/ACA_rnp_Gar1/Naf1. IPR021154. H/ACA_rnp_Gar1_euk. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23237. PTHR23237. 1 hit.
Pfam	PF04410. Gar1. 1 hit. [Graphical view]
ProDom	PD020235. Gar1_RNA_bd. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF50447. Translat_factor. 1 hit.
ProtoNet	Search...
Other
NextBio	703745.

Entry information

Entry name

GAR1_RAT

Accession

Primary (citable) accession number: Q6AYA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	September 13, 2004
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program