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Q6AYA1 (GAR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
Nucleolar protein family A member 1
snoRNP protein GAR1
Gene names
Name:Gar1
Synonyms:Nola1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme By similarity.

Subunit structure

Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) By similarity.

Subcellular location

Nucleusnucleolus By similarity. NucleusCajal body By similarity. Note: Also localized to Cajal bodies (coiled bodies) By similarity.

Domain

Interaction with SMN1 requires at least one of the RGG-box regions By similarity.

Sequence similarities

Belongs to the GAR1 family.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentNucleus
   DomainRepeat
   LigandRNA-binding
   Molecular functionRibonucleoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpseudouridine synthesis

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCajal body

Inferred from electronic annotation. Source: UniProtKB-SubCell

box H/ACA snoRNP complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226H/ACA ribonucleoprotein complex subunit 1
PRO_0000208554

Regions

Region4 – 5956RGG-box 1
Region171 – 22656RGG-box 2

Sequences

Sequence LengthMass (Da)Tools
Q6AYA1 [UniParc].

Last modified September 13, 2004. Version 1.
Checksum: B81ADE63E02F151D

FASTA22623,011
        10         20         30         40         50         60 
MSFRGGGRGG FNRGGGGGGF NRGGGSNNHF RGGGGGGGGG GNFRGGGRGG FGRGGGRGGF 

        70         80         90        100        110        120 
NKFQDQGPPE RVVLLGEFMH PCEDDIVCKC TTEENKVPYF NAPVYLENKE QIGKVDEIFG 

       130        140        150        160        170        180 
QLRDFYFSVK LSENMKASSF KKLQKFYIDP YKLLPLQRFL PRPPGEKGPP RGGGGGGGGG 

       190        200        210        220 
RGGRGGGRGG GGRGGGRGGG FRGGRGGGGG FRGGRGGGGG FRGRGH 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC079131 mRNA. Translation: AAH79131.1.
RefSeqNP_001019477.1. NM_001024306.1.
UniGeneRn.15522.

3D structure databases

ProteinModelPortalQ6AYA1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013149.

Proteomic databases

PaxDbQ6AYA1.
PRIDEQ6AYA1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013149; ENSRNOP00000013149; ENSRNOG00000009877.
GeneID499709.
KEGGrno:499709.
UCSCRGD:1563995. rat.

Organism-specific databases

CTD54433.
RGD1563995. Gar1.

Phylogenomic databases

eggNOGCOG3277.
GeneTreeENSGT00730000111223.
InParanoidQ6AYA1.
KOK11128.
OMATHENKVP.
OrthoDBEOG78H3WG.
TreeFamTF350747.

Gene expression databases

GenevestigatorQ6AYA1.

Family and domain databases

InterProIPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERPTHR23237. PTHR23237. 1 hit.
PfamPF04410. Gar1. 1 hit.
[Graphical view]
ProDomPD020235. Gar1_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF50447. SSF50447. 1 hit.
ProtoNetSearch...

Other

NextBio703745.
PROQ6AYA1.

Entry information

Entry nameGAR1_RAT
AccessionPrimary (citable) accession number: Q6AYA1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families