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Protein

H/ACA ribonucleoprotein complex subunit 1

Gene

Gar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. pseudouridine synthesis Source: InterPro
  2. rRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
Nucleolar protein family A member 1
snoRNP protein GAR1
Gene namesi
Name:Gar1
Synonyms:Nola1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1563995. Gar1.

Subcellular locationi

Nucleusnucleolus By similarity. NucleusCajal body By similarity
Note: Also localized to Cajal bodies (coiled bodies).By similarity

GO - Cellular componenti

  1. box H/ACA snoRNP complex Source: InterPro
  2. Cajal body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226H/ACA ribonucleoprotein complex subunit 1PRO_0000208554Add
BLAST

Proteomic databases

PaxDbiQ6AYA1.
PRIDEiQ6AYA1.

Expressioni

Gene expression databases

GenevestigatoriQ6AYA1.

Interactioni

Subunit structurei

Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013149.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 5956RGG-box 1Add
BLAST
Regioni171 – 22656RGG-box 2Add
BLAST

Domaini

Interaction with SMN1 requires at least one of the RGG-box regions.By similarity

Sequence similaritiesi

Belongs to the GAR1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3277.
GeneTreeiENSGT00730000111223.
InParanoidiQ6AYA1.
KOiK11128.
OMAiNKTAVGK.
OrthoDBiEOG78H3WG.
TreeFamiTF350747.

Family and domain databases

InterProiIPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR23237. PTHR23237. 1 hit.
PfamiPF04410. Gar1. 1 hit.
[Graphical view]
ProDomiPD020235. Gar1_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AYA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRGGGRGG FNRGGGGGGF NRGGGSNNHF RGGGGGGGGG GNFRGGGRGG
60 70 80 90 100
FGRGGGRGGF NKFQDQGPPE RVVLLGEFMH PCEDDIVCKC TTEENKVPYF
110 120 130 140 150
NAPVYLENKE QIGKVDEIFG QLRDFYFSVK LSENMKASSF KKLQKFYIDP
160 170 180 190 200
YKLLPLQRFL PRPPGEKGPP RGGGGGGGGG RGGRGGGRGG GGRGGGRGGG
210 220
FRGGRGGGGG FRGGRGGGGG FRGRGH
Length:226
Mass (Da):23,011
Last modified:September 13, 2004 - v1
Checksum:iB81ADE63E02F151D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079131 mRNA. Translation: AAH79131.1.
RefSeqiNP_001019477.1. NM_001024306.1.
UniGeneiRn.15522.

Genome annotation databases

EnsembliENSRNOT00000013149; ENSRNOP00000013149; ENSRNOG00000009877.
GeneIDi499709.
KEGGirno:499709.
UCSCiRGD:1563995. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079131 mRNA. Translation: AAH79131.1.
RefSeqiNP_001019477.1. NM_001024306.1.
UniGeneiRn.15522.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013149.

Proteomic databases

PaxDbiQ6AYA1.
PRIDEiQ6AYA1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013149; ENSRNOP00000013149; ENSRNOG00000009877.
GeneIDi499709.
KEGGirno:499709.
UCSCiRGD:1563995. rat.

Organism-specific databases

CTDi54433.
RGDi1563995. Gar1.

Phylogenomic databases

eggNOGiCOG3277.
GeneTreeiENSGT00730000111223.
InParanoidiQ6AYA1.
KOiK11128.
OMAiNKTAVGK.
OrthoDBiEOG78H3WG.
TreeFamiTF350747.

Miscellaneous databases

NextBioi703745.
PROiQ6AYA1.

Gene expression databases

GenevestigatoriQ6AYA1.

Family and domain databases

InterProiIPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR23237. PTHR23237. 1 hit.
PfamiPF04410. Gar1. 1 hit.
[Graphical view]
ProDomiPD020235. Gar1_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGAR1_RAT
AccessioniPrimary (citable) accession number: Q6AYA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: September 13, 2004
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.