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Protein

General transcription factor IIF subunit 1

Gene

Gtf2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 1
Alternative name(s):
Transcription initiation factor IIF subunit alpha
Short name:
TFIIF-alpha
Gene namesi
Name:Gtf2f1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1359646. Gtf2f1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 508507General transcription factor IIF subunit 1PRO_0000260323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei217 – 2171PhosphoserineBy similarity
Modified residuei218 – 2181PhosphoserineBy similarity
Modified residuei221 – 2211PhosphoserineCombined sources
Modified residuei224 – 2241PhosphoserineCombined sources
Modified residuei331 – 3311PhosphothreonineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei380 – 3801PhosphoserineBy similarity
Modified residuei381 – 3811PhosphoserineBy similarity
Modified residuei385 – 3851PhosphoserineCombined sources
Modified residuei389 – 3891PhosphothreonineCombined sources
Modified residuei391 – 3911PhosphoserineBy similarity
Modified residuei407 – 4071N6-acetyllysineBy similarity
Modified residuei431 – 4311PhosphoserineBy similarity
Modified residuei433 – 4331PhosphoserineCombined sources
Modified residuei436 – 4361PhosphoserineBy similarity
Modified residuei437 – 4371PhosphothreonineBy similarity
Modified residuei440 – 4401PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AY96.
PRIDEiQ6AY96.

PTM databases

iPTMnetiQ6AY96.
PhosphoSiteiQ6AY96.

Expressioni

Gene expression databases

GenevisibleiQ6AY96. RN.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi261260. 1 interaction.
STRINGi10116.ENSRNOP00000064830.

Structurei

3D structure databases

ProteinModelPortaliQ6AY96.
SMRiQ6AY96. Positions 7-168, 440-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi283 – 35068Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the TFIIF alpha subunit family.Curated

Phylogenomic databases

eggNOGiKOG2393. Eukaryota.
ENOG410XSRW. LUCA.
GeneTreeiENSGT00440000038032.
HOVERGENiHBG000729.
InParanoidiQ6AY96.
KOiK03138.
OMAiSALFMTK.
OrthoDBiEOG7GXPBV.
PhylomeDBiQ6AY96.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR008851. TFIIF-alpha.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05793. TFIIF_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50916. SSF50916. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AY96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALGSSSQN VTEYVVRVPK NTAKRYNIMA FNAADKVNFA TWNQARLERD
60 70 80 90 100
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL
110 120 130 140 150
RVNGKSGRKF KGIKKGGVTE NTAYYIFTQC ADGAFEAFPV QNWYNFTPLA
160 170 180 190 200
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRSRK
210 220 230 240 250
KPSELRIHDL EDDLEMSSDA SDASGEEGSR ASKAKKKAPV TKAGRKKKKK
260 270 280 290 300
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SPDEAEGKPK VPQQEDGPKG
310 320 330 340 350
VDEQSESSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSDDSDSSEE
360 370 380 390 400
SDIDSETSSA LFMAKKKTPP KRERKPSGGS SKGTSRPGTP SAEAASTSST
410 420 430 440 450
LRAAASKLEQ GKRTSETPAA KRLRMDTGPQ SLSGKSTPSS GDVQVTEDAV
460 470 480 490 500
RRYLTRKPMT TKDLLKKFQT KKTGLSSEQT VNVLAQILKR LNPERKMIGD

KMHFSLKE
Length:508
Mass (Da):57,181
Last modified:September 13, 2004 - v1
Checksum:i9114273EF681B7B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079136 mRNA. Translation: AAH79136.1.
RefSeqiNP_001007712.1. NM_001007711.1.
XP_006244383.1. XM_006244321.2.
UniGeneiRn.98836.

Genome annotation databases

EnsembliENSRNOT00000073683; ENSRNOP00000064830; ENSRNOG00000047134.
GeneIDi316123.
KEGGirno:316123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079136 mRNA. Translation: AAH79136.1.
RefSeqiNP_001007712.1. NM_001007711.1.
XP_006244383.1. XM_006244321.2.
UniGeneiRn.98836.

3D structure databases

ProteinModelPortaliQ6AY96.
SMRiQ6AY96. Positions 7-168, 440-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi261260. 1 interaction.
STRINGi10116.ENSRNOP00000064830.

PTM databases

iPTMnetiQ6AY96.
PhosphoSiteiQ6AY96.

Proteomic databases

PaxDbiQ6AY96.
PRIDEiQ6AY96.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073683; ENSRNOP00000064830; ENSRNOG00000047134.
GeneIDi316123.
KEGGirno:316123.

Organism-specific databases

CTDi2962.
RGDi1359646. Gtf2f1.

Phylogenomic databases

eggNOGiKOG2393. Eukaryota.
ENOG410XSRW. LUCA.
GeneTreeiENSGT00440000038032.
HOVERGENiHBG000729.
InParanoidiQ6AY96.
KOiK03138.
OMAiSALFMTK.
OrthoDBiEOG7GXPBV.
PhylomeDBiQ6AY96.

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ6AY96.

Gene expression databases

GenevisibleiQ6AY96. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR008851. TFIIF-alpha.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05793. TFIIF_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50916. SSF50916. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221; SER-224; SER-385; THR-389 AND SER-433, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2FA_RAT
AccessioniPrimary (citable) accession number: Q6AY96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 13, 2004
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.