Q6AY63 (NUDT5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-sugar pyrophosphatase
EC=3.6.1.-
EC=3.6.1.13

Alternative name(s):
Nucleoside diphosphate-linked moiety X motif 5
Short name=Nudix motif 5

Gene names

Name:

Nudt5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity By similarity.
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. ADP-sugar + H2O = AMP + alpha-D-aldose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ribonucleoside diphosphate catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	ADP-sugar pyrophosphatase		PRO_0000250703
Regions
	Domain	57 – 197	141	Nudix hydrolase
	Region	46 – 47	2	Substrate binding; shared with dimeric partner By similarity
	Motif	97 – 118	22	Nudix box
Sites
	Metal binding	96	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	112	1	Magnesium 2 By similarity
	Metal binding	112	1	Magnesium 3 By similarity
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	116	1	Magnesium 3 By similarity
	Metal binding	166	1	Magnesium 3 By similarity
	Binding site	28	1	Substrate By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	98	1	Substrate; via amide nitrogen By similarity
	Binding site	133	1	Substrate By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	10	1	Phosphoserine By similarity
	Modified residue	42	1	N6-acetyllysine By similarity
	Modified residue	210	1	N6-acetyllysine By similarity
	Modified residue	218	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AY63 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 87AFCD2BDD6E4B04	FASTA	219	24,117
	10 20 30 40 50 60 METQEPKDSS PPTEQRILSE ELISEGKWVK FEKTTYMDPT GKTRTWETVK LTTRKGKSAD 70 80 90 100 110 120 AVSVIPVLQR TLHHECIVLV KQFRPPMGGY CLEFPAGLIE DGESPEAAAL RELEEETGYK 130 140 150 160 170 180 GDIAECSPAV CMDPGLSNCT THVVTVTING DDAGNVRPKP KPGDGEFVEV ISLPKNDLLT 190 200 210 RLDALGAEDR LTVDARVYAY ALALKHANAK PFEVPFLKF

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079176 mRNA. Translation: AAH79176.1.
IPI	IPI00364928.
RefSeq	NP_001007734.1. NM_001007733.1.
UniGene	Rn.55388.
3D structure databases
ProteinModelPortal	Q6AY63.
SMR	Q6AY63. Positions 14-219.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AY63.
Proteomic databases
PRIDE	Q6AY63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000068551; ENSRNOP00000060464; ENSRNOG00000017741.
GeneID	361274.
KEGG	rno:361274.
UCSC	NM_001007733. rat.
Organism-specific databases
CTD	11164.
RGD	1359284. Nudt5.
Phylogenomic databases
eggNOG	roNOG08100.
GeneTree	ENSGT00390000006280.
HOVERGEN	HBG052691.
InParanoid	Q6AY63.
OrthoDB	EOG46Q6TG.
PhylomeDB	Q6AY63.
Gene expression databases
ArrayExpress	Q6AY63.
Genevestigator	Q6AY63.
GermOnline	ENSRNOG00000017741. Rattus norvegicus.
Family and domain databases
InterPro	IPR020476. Nudix_hydrolase. IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
KO	K13987.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR00502. NUDIXFAMILY.
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	675785.

Entry information

Entry name

NUDT5_RAT

Accession

Primary (citable) accession number: Q6AY63

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	September 13, 2004
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program