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Protein

WD repeat domain phosphoinositide-interacting protein 2

Gene

Wipi2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 2
Short name:
WIPI-2
Gene namesi
Name:Wipi2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1359154 Wipi2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514421 – 445WD repeat domain phosphoinositide-interacting protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6AY57
PRIDEiQ6AY57

PTM databases

iPTMnetiQ6AY57
PhosphoSitePlusiQ6AY57

Expressioni

Gene expression databases

BgeeiENSRNOG00000001114
GenevisibleiQ6AY57 RN

Interactioni

Subunit structurei

Interacts with TECPR1, ATG16L1 and ATG5.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001476

Structurei

3D structure databases

ProteinModelPortaliQ6AY57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati182 – 222WD 1Sequence analysisAdd BLAST41
Repeati228 – 267WD 2Sequence analysisAdd BLAST40
Repeati311 – 349WD 3Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi223 – 226FRRG motifBy similarity4

Domaini

The FRRG motif is required for recruitment to PtdIns3P.By similarity

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110 Eukaryota
ENOG410XP6Y LUCA
GeneTreeiENSGT00730000110845
HOGENOMiHOG000217544
HOVERGENiHBG056639
InParanoidiQ6AY57
KOiK17908
OMAiPNAICAL
OrthoDBiEOG091G08ZS
PhylomeDBiQ6AY57
TreeFamiTF314879

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR036322 WD40_repeat_dom_sf
IPR032911 WIPI2
PANTHERiPTHR11227:SF27 PTHR11227:SF27, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50978 SSF50978, 2 hits

Sequencei

Sequence statusi: Complete.

Q6AY57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLASQSGEA GAGQLLFANF NQDNTSLAVG SKSGYKFFSL SSVDKLEQIY
60 70 80 90 100
ECTDTEDVCI VERLFSSSLV AIVSLKAPRK LKVCHFKKGT EICNYSYSNT
110 120 130 140 150
ILAVKLNRQR LIVCLEESLY IHNIRDMKVL HTIRETPPNP AGLCALSINN
160 170 180 190 200
DNCYLAYPGS ATIGEVQVFD TINLRAANMI PAHDSPLAAL AFDASGTKLA
210 220 230 240 250
TASEKGTVIR VFSIPEGQKL FEFRRGVKRC VSICSLAFSM DGMFLSASSN
260 270 280 290 300
TETVHIFKLE AVREKPPEEP TTWTGYFGKV LMASTSYLPS QVTEMFNQGR
310 320 330 340 350
AFATVRLPFC GHKNICSLTT IQKIPRLLVG ASDGYLYMYN LDPQEGGECA
360 370 380 390 400
LMRQHRLDGS METTSEIVDS ASHDCPLVTQ TYGTAAAKGA YVPSSPTRLG
410 420 430 440
KGQDANLEAY TDDLGAVGGA CLEDEASALR LDEDSEHPPM ILRTD
Length:445
Mass (Da):48,519
Last modified:September 13, 2004 - v1
Checksum:i884A8489F2AC399F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079184 mRNA Translation: AAH79184.1
RefSeqiNP_001007616.1, NM_001007615.1
UniGeneiRn.163194

Genome annotation databases

EnsembliENSRNOT00000001476; ENSRNOP00000001476; ENSRNOG00000001114
GeneIDi288498
KEGGirno:288498
UCSCiRGD:1359154 rat

Similar proteinsi

Entry informationi

Entry nameiWIPI2_RAT
AccessioniPrimary (citable) accession number: Q6AY57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2004
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health