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Protein

Deoxyhypusine synthase

Gene

Dhps

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei137 – 1371NADBy similarity
Binding sitei238 – 2381NADBy similarity
Binding sitei243 – 2431SpermidineBy similarity
Binding sitei283 – 2831NAD; via amide nitrogenBy similarity
Binding sitei288 – 2881SpermidineBy similarity
Active sitei329 – 3291NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1095NADBy similarity
Nucleotide bindingi131 – 1333NADBy similarity
Nucleotide bindingi308 – 3092NADBy similarity
Nucleotide bindingi342 – 3432NADBy similarity

GO - Molecular functioni

  • deoxyhypusine synthase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:Dhps
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1303326. Dhps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Deoxyhypusine synthasePRO_0000134470Add
BLAST

Proteomic databases

PaxDbiQ6AY53.
PRIDEiQ6AY53.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004219.
GenevisibleiQ6AY53. RN.

Interactioni

Protein-protein interaction databases

IntActiQ6AY53. 1 interaction.
STRINGi10116.ENSRNOP00000005656.

Chemistry

BindingDBiQ6AY53.

Structurei

3D structure databases

ProteinModelPortaliQ6AY53.
SMRiQ6AY53. Positions 9-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1372Spermidine bindingBy similarity
Regioni314 – 3163Spermidine bindingBy similarity
Regioni323 – 3297Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
HOVERGENiHBG000852.
InParanoidiQ6AY53.
KOiK00809.
OMAiTTAVPWD.
OrthoDBiEOG091G09FV.
PhylomeDBiQ6AY53.
TreeFamiTF300625.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AY53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGTPSGAAP SSALAAVLKH SSALPPESAQ VQGYDFNRGV DYHALLEAYG
60 70 80 90 100
TTGFQATNFG RAVQQVNAMI EKKLEPLAVD EDHHEDLTQS RRPLTGCTIF
110 120 130 140 150
LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE
160 170 180 190 200
FSLRGKELRE NGINRIGNLL VPNDNYCKFE DWLMPILDQM VQEQNTEGVK
210 220 230 240 250
WTPSKMISRL GKEINNPESV YYWAHKNHIP VLSPALTDGS LGDMIFFHSY
260 270 280 290 300
KNPGLVLDIV EDLRLINMQA IFAKRTGMII LGGGVVKHHI ANANLMRNGA
310 320 330 340 350
DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RMDAQPVKVY ADASLVFPLL
360
VAETFAQKAD AFRAEKNED
Length:369
Mass (Da):40,705
Last modified:September 13, 2004 - v1
Checksum:i8707A21A9E8D5202
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079188 mRNA. Translation: AAH79188.1.
RefSeqiNP_001004207.1. NM_001004207.1.
UniGeneiRn.105891.

Genome annotation databases

EnsembliENSRNOT00000005656; ENSRNOP00000005656; ENSRNOG00000004219.
GeneIDi288923.
KEGGirno:288923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079188 mRNA. Translation: AAH79188.1.
RefSeqiNP_001004207.1. NM_001004207.1.
UniGeneiRn.105891.

3D structure databases

ProteinModelPortaliQ6AY53.
SMRiQ6AY53. Positions 9-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6AY53. 1 interaction.
STRINGi10116.ENSRNOP00000005656.

Chemistry

BindingDBiQ6AY53.
ChEMBLiCHEMBL2539.

Proteomic databases

PaxDbiQ6AY53.
PRIDEiQ6AY53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005656; ENSRNOP00000005656; ENSRNOG00000004219.
GeneIDi288923.
KEGGirno:288923.

Organism-specific databases

CTDi1725.
RGDi1303326. Dhps.

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
HOVERGENiHBG000852.
InParanoidiQ6AY53.
KOiK00809.
OMAiTTAVPWD.
OrthoDBiEOG091G09FV.
PhylomeDBiQ6AY53.
TreeFamiTF300625.

Enzyme and pathway databases

UniPathwayiUPA00354.
ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ6AY53.

Gene expression databases

BgeeiENSRNOG00000004219.
GenevisibleiQ6AY53. RN.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_RAT
AccessioniPrimary (citable) accession number: Q6AY53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: September 13, 2004
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.