Q6AY47 (FICD_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine monophosphate-protein transferase FICD
EC=2.7.7.n1

Alternative name(s):
AMPylator FICD
FIC domain-containing protein

Gene names

Name:

Ficd

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins By similarity.
Catalytic activity	ATP + [protein] = diphosphate + [protein]-AMP.
Enzyme regulation	Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-234 binds ATP and competes with ATP-binding at Arg-374, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-234, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain By similarity.
Subunit structure	Interacts with HD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The fido domain mediates the adenylyltransferase activity By similarity.
Sequence similarities	Belongs to the fic family. Contains 1 fido domain. Contains 2 TPR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Adenosine monophosphate-protein transferase FICD		PRO_0000317303
Regions
	Transmembrane	24 – 44	21	Helical; Potential
	Repeat	106 – 139	34	TPR 1
	Repeat	140 – 173	34	TPR 2
	Domain	285 – 420	136	Fido
	Nucleotide binding	260 – 261	2	ATP By similarity
	Nucleotide binding	368 – 370	3	ATP By similarity
	Motif	230 – 235	6	Inhibitory (S/T)XXXE(G/N) motif
Sites
	Binding site	234	1	ATP By similarity
	Binding site	374	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AY47 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: CA59D9A4F7D559F9	FASTA	458	51,754
	10 20 30 40 50 60 MILMPMASVV AVAEPKWVSV WGRFLWMTLL SMALGSLLAL LLPLGAVEEQ CLAVLRGFHL 70 80 90 100 110 120 LRSKLDRAQH VVTKCTSPST ELSVTSRDAG LLTVKTKASP AGKLEAKAAL NQALEMKRQG 130 140 150 160 170 180 KRGKAHKLFL HALKMDPGFV DALNELGIFS EEDKDIIQAD YLYTRALTIS PFHEKALINR 190 200 210 220 230 240 DRTLPLVEEI DQRYFSVLDS KVRKVMSIPK GSSALRRVME ETYYHHIYHT VAIEGNTLTL 250 260 270 280 290 300 AEIRHILETR YAVPGKSLEE QNEVIGMHAA MKYINSTLVS RIGSVTIDHM LEIHRRVLGY 310 320 330 340 350 360 VDPVEAGRFR RTQVLVGHHI PPHPRDVEKQ MQEFTQWLNS EDAMNLHPVE FAALAHYKLV 370 380 390 400 410 420 YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSEYY HVLEVANEGD VRPFIRFIAK 430 440 450 CTEVTLDTLL LATTEYSAAL PEAQPNHSGF KETLPVRP

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079197 mRNA. Translation: AAH79197.1.
IPI	IPI00371233.
RefSeq	NP_001010946.1. NM_001010946.1.
UniGene	Rn.162153.
3D structure databases
ProteinModelPortal	Q6AY47.
ModBase	Search...
Proteomic databases
PRIDE	Q6AY47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000892; ENSRNOP00000000892; ENSRNOG00000000703.
GeneID	288741.
KEGG	rno:288741.
UCSC	RGD:1359391. rat.
Organism-specific databases
CTD	11153.
RGD	1359391. Ficd.
Phylogenomic databases
eggNOG	COG3177.
GeneTree	ENSGT00390000008873.
HOGENOM	HOG000008059.
HOVERGEN	HBG095654.
InParanoid	Q6AY47.
OMA	MKYINTT.
OrthoDB	EOG4RR6HD.
Gene expression databases
Genevestigator	Q6AY47.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR003812. Fido. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF02661. Fic. 1 hit. [Graphical view]
SUPFAM	SSF140931. SSF140931. 1 hit.
PROSITE	PS51459. FIDO. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	628637.

Entry information

Entry name

FICD_RAT

Accession

Primary (citable) accession number: Q6AY47

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2004
Last modified:	March 6, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program