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Protein

Adenosine monophosphate-protein transferase FICD

Gene

Ficd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins.By similarity

Catalytic activityi

ATP + [protein] = diphosphate + [protein]-AMP.

Enzyme regulationi

Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-234 binds ATP and competes with ATP-binding at Arg-374, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-234, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei234 – 2341ATPBy similarity
Binding sitei374 – 3741ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi260 – 2612ATPBy similarity
Nucleotide bindingi368 – 3703ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine monophosphate-protein transferase FICD (EC:2.7.7.n1)
Alternative name(s):
AMPylator FICD
FIC domain-containing protein
Gene namesi
Name:Ficd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1359391. Ficd.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Adenosine monophosphate-protein transferase FICDPRO_0000317303Add
BLAST

Proteomic databases

PaxDbiQ6AY47.
PRIDEiQ6AY47.

Expressioni

Gene expression databases

GenevisibleiQ6AY47. RN.

Interactioni

Subunit structurei

Interacts with HD.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000892.

Structurei

3D structure databases

ProteinModelPortaliQ6AY47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati106 – 13934TPR 1Add
BLAST
Repeati140 – 17334TPR 2Add
BLAST
Domaini285 – 420136FidoPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi230 – 2356Inhibitory (S/T)XXXE(G/N) motif

Domaini

The fido domain mediates the adenylyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the fic family.Curated
Contains 1 fido domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3824. Eukaryota.
COG3177. LUCA.
GeneTreeiENSGT00390000008873.
HOGENOMiHOG000008059.
HOVERGENiHBG095654.
InParanoidiQ6AY47.
OMAiNYAALAH.
OrthoDBiEOG7FJH0F.
PhylomeDBiQ6AY47.
TreeFamiTF314692.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR003812. Fido.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
SSF48452. SSF48452. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AY47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILMPMASVV AVAEPKWVSV WGRFLWMTLL SMALGSLLAL LLPLGAVEEQ
60 70 80 90 100
CLAVLRGFHL LRSKLDRAQH VVTKCTSPST ELSVTSRDAG LLTVKTKASP
110 120 130 140 150
AGKLEAKAAL NQALEMKRQG KRGKAHKLFL HALKMDPGFV DALNELGIFS
160 170 180 190 200
EEDKDIIQAD YLYTRALTIS PFHEKALINR DRTLPLVEEI DQRYFSVLDS
210 220 230 240 250
KVRKVMSIPK GSSALRRVME ETYYHHIYHT VAIEGNTLTL AEIRHILETR
260 270 280 290 300
YAVPGKSLEE QNEVIGMHAA MKYINSTLVS RIGSVTIDHM LEIHRRVLGY
310 320 330 340 350
VDPVEAGRFR RTQVLVGHHI PPHPRDVEKQ MQEFTQWLNS EDAMNLHPVE
360 370 380 390 400
FAALAHYKLV YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSEYY
410 420 430 440 450
HVLEVANEGD VRPFIRFIAK CTEVTLDTLL LATTEYSAAL PEAQPNHSGF

KETLPVRP
Length:458
Mass (Da):51,754
Last modified:September 13, 2004 - v1
Checksum:iCA59D9A4F7D559F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079197 mRNA. Translation: AAH79197.1.
RefSeqiNP_001010946.1. NM_001010946.1.
XP_008767542.1. XM_008769320.1.
XP_008767543.1. XM_008769321.1.
UniGeneiRn.162153.

Genome annotation databases

EnsembliENSRNOT00000082809; ENSRNOP00000073778; ENSRNOG00000059799.
GeneIDi288741.
KEGGirno:288741.
UCSCiRGD:1359391. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079197 mRNA. Translation: AAH79197.1.
RefSeqiNP_001010946.1. NM_001010946.1.
XP_008767542.1. XM_008769320.1.
XP_008767543.1. XM_008769321.1.
UniGeneiRn.162153.

3D structure databases

ProteinModelPortaliQ6AY47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000892.

Proteomic databases

PaxDbiQ6AY47.
PRIDEiQ6AY47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000082809; ENSRNOP00000073778; ENSRNOG00000059799.
GeneIDi288741.
KEGGirno:288741.
UCSCiRGD:1359391. rat.

Organism-specific databases

CTDi11153.
RGDi1359391. Ficd.

Phylogenomic databases

eggNOGiKOG3824. Eukaryota.
COG3177. LUCA.
GeneTreeiENSGT00390000008873.
HOGENOMiHOG000008059.
HOVERGENiHBG095654.
InParanoidiQ6AY47.
OMAiNYAALAH.
OrthoDBiEOG7FJH0F.
PhylomeDBiQ6AY47.
TreeFamiTF314692.

Miscellaneous databases

NextBioi628637.
PROiQ6AY47.

Gene expression databases

GenevisibleiQ6AY47. RN.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR003812. Fido.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02661. Fic. 1 hit.
[Graphical view]
SUPFAMiSSF140931. SSF140931. 1 hit.
SSF48452. SSF48452. 1 hit.
PROSITEiPS51459. FIDO. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiFICD_RAT
AccessioniPrimary (citable) accession number: Q6AY47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2004
Last modified: December 9, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.