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Protein

Adenosine monophosphate-protein transferase FICD

Gene

Ficd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins.By similarity

Catalytic activityi

ATP + [protein] = diphosphate + [protein]-AMP.

Enzyme regulationi

Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-234 binds ATP and competes with ATP-binding at Arg-374, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-234, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234ATPBy similarity1
Binding sitei374ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi260 – 261ATPBy similarity2
Nucleotide bindingi368 – 370ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine monophosphate-protein transferase FICD (EC:2.7.7.n1)
Alternative name(s):
AMPylator FICD
FIC domain-containing protein
Gene namesi
Name:Ficd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1359391. Ficd.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003173031 – 458Adenosine monophosphate-protein transferase FICDAdd BLAST458

Proteomic databases

PaxDbiQ6AY47.

Expressioni

Gene expression databases

BgeeiENSRNOG00000059799.
GenevisibleiQ6AY47. RN.

Interactioni

Subunit structurei

Interacts with HD.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6AY47.
SMRiQ6AY47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati106 – 139TPR 1Add BLAST34
Repeati140 – 173TPR 2Add BLAST34
Domaini285 – 420FidoPROSITE-ProRule annotationAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 235Inhibitory (S/T)XXXE(G/N) motif6

Domaini

The fido domain mediates the adenylyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the fic family.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3824. Eukaryota.
COG3177. LUCA.
GeneTreeiENSGT00390000008873.
HOGENOMiHOG000008059.
HOVERGENiHBG095654.
InParanoidiQ6AY47.
OMAiNYAALAH.
OrthoDBiEOG091G0GN7.
PhylomeDBiQ6AY47.
TreeFamiTF314692.

Family and domain databases

Gene3Di1.10.3290.10. 1 hit.
1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR003812. Fido.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
PfamiView protein in Pfam
PF02661. Fic. 1 hit.
SUPFAMiSSF140931. SSF140931. 1 hit.
SSF48452. SSF48452. 1 hit.
PROSITEiView protein in PROSITE
PS51459. FIDO. 1 hit.
PS50293. TPR_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AY47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILMPMASVV AVAEPKWVSV WGRFLWMTLL SMALGSLLAL LLPLGAVEEQ
60 70 80 90 100
CLAVLRGFHL LRSKLDRAQH VVTKCTSPST ELSVTSRDAG LLTVKTKASP
110 120 130 140 150
AGKLEAKAAL NQALEMKRQG KRGKAHKLFL HALKMDPGFV DALNELGIFS
160 170 180 190 200
EEDKDIIQAD YLYTRALTIS PFHEKALINR DRTLPLVEEI DQRYFSVLDS
210 220 230 240 250
KVRKVMSIPK GSSALRRVME ETYYHHIYHT VAIEGNTLTL AEIRHILETR
260 270 280 290 300
YAVPGKSLEE QNEVIGMHAA MKYINSTLVS RIGSVTIDHM LEIHRRVLGY
310 320 330 340 350
VDPVEAGRFR RTQVLVGHHI PPHPRDVEKQ MQEFTQWLNS EDAMNLHPVE
360 370 380 390 400
FAALAHYKLV YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSEYY
410 420 430 440 450
HVLEVANEGD VRPFIRFIAK CTEVTLDTLL LATTEYSAAL PEAQPNHSGF

KETLPVRP
Length:458
Mass (Da):51,754
Last modified:September 13, 2004 - v1
Checksum:iCA59D9A4F7D559F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079197 mRNA. Translation: AAH79197.1.
RefSeqiNP_001010946.1. NM_001010946.1.
XP_008767542.1. XM_008769320.2.
XP_008767543.1. XM_008769321.2.
UniGeneiRn.162153.

Genome annotation databases

EnsembliENSRNOT00000082809; ENSRNOP00000073778; ENSRNOG00000059799.
GeneIDi288741.
KEGGirno:288741.
UCSCiRGD:1359391. rat.

Similar proteinsi

Entry informationi

Entry nameiFICD_RAT
AccessioniPrimary (citable) accession number: Q6AY47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2004
Last modified: August 30, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families