Reviewed, UniProtKB/Swiss-Prot Q6AY39 (B3GL1_RAT)

Names and origin

Protein names

Recommended name:
    UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
    EC=2.4.1.79
Alternative name(s):
    Beta-3-GalNAc-T1
    Beta-1,3-galactosyltransferase 3
      Short name=Beta-1,3-GalTase 3
      Short name=Beta3Gal-T3
      Short name=b3Gal-T3
    Beta-3-Gx-T3
    Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase
    UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase
    Globoside synthase

Gene names

Name:	B3galnt1
Synonyms:	B3galt3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers N-acetylgalactosamine onto globotriaosylceramide By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Cofactor	Magnesium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1		PRO_0000219158
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 43	23	Signal-anchor for type II membrane protein Potential
	Topological domain	44 – 331	288	Lumenal Potential
Amino acid modifications
	Modified residue	140	1	Phosphotyrosine By similarity
	Modified residue	152	1	Phosphotyrosine By similarity
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6AY39-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A0BF5A919A99339F	FASTA	331	39,215
	10 20 30 40 50 60 MAPAVLTAIP NRMSLRSLKW SLLLLSLLSF LVIWYLSLPH YNVIERVNWM YFYEYEPIYR 70 80 90 100 110 120 QDFQFTLREH SNCSQQNPFL VILVTSRPSD VKARQAIRVT WGEKKTWWGH EVLTFFLLGQ 130 140 150 160 170 180 EAEREDKVLA LSLEDEHALY GDIIRQDFLD TYNNLTLKTI MAFRWVIEFC PNAKYVMKTD 190 200 210 220 230 240 TDVFINTGNL VKYLLNLNHS EKFFTGYPLI ENYSYRGFFH KNHISYQEYP FKVFPPYCSG 250 260 270 280 290 300 LGYIMSGDLV PKIYEMMGHV KPIKFEDVYV GICLNLLKVD IHIPEDTNLF FLFRIHLDVC 310 320 330 QLRRVIAAHG FSSKEIITFW QVMLRNTTCH Y

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079206 mRNA. Translation: AAH79206.1.
IPI	IPI00213749.
RefSeq	NP_001013176.1.
UniGene	Rn.3666
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Genome annotation databases
Ensembl	ENSRNOT00000016012; ENSRNOP00000016012; ENSRNOG00000012019; Rattus norvegicus. [Genome view]
GeneID	310508.
KEGG	rno:310508.
NMPDR	fig|10116.3.peg.16197.
UCSC	NM_001013158. rat.
Organism-specific databases
CTD	310508.
RGD	1304797. B3galnt1.
Phylogenomic databases
HOVERGEN	Q6AY39.
OMA	RNTTCHY.
Enzyme and pathway databases
BRENDA	2.4.1.79. 248.
Gene expression databases
ArrayExpress	Q6AY39.
Genevestigator	Q6AY39.
GermOnline	ENSRNOG00000012019. Rattus norvegicus.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	662077.

Entry information

Entry name

B3GL1_RAT

Accession

Primary (citable) accession number: Q6AY39

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	September 13, 2004
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)