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Protein

Transmembrane emp24 domain-containing protein 3

Gene

Tmed3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 3
Alternative name(s):
p24 family protein gamma-4
Short name:
p24gamma4
Gene namesi
Name:Tmed3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1303327. Tmed3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 184154LumenalSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 22116CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 221191Transmembrane emp24 domain-containing protein 3PRO_0000010386Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031Dimethylated arginine1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ6AY25.
PRIDEiQ6AY25.

PTM databases

iPTMnetiQ6AY25.
PhosphoSiteiQ6AY25.

Expressioni

Gene expression databases

GenevisibleiQ6AY25. RN.

Interactioni

Subunit structurei

Monomer in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED3; however, there are conflicting reports on the interaction (By similarity). Interacts with GORASP1 and GORASP2.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 12483GOLDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi208 – 22114COPI vesicle coat-bindingSequence analysisAdd
BLAST
Motifi208 – 2092COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ6AY25.
OMAiGRVSYWS.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ6AY25.
TreeFamiTF313000.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AY25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNEVPRASS FQMLMLLLLL LLLRAERLRG AELTFELPDN AKQCFHEEVE
60 70 80 90 100
QGVKFSLDYQ VITGGHYDVD CYVEDPMGNI IYRETKKQYD SFTYKTEVKG
110 120 130 140 150
VYRFCFSNEF STFSHKTVYF DFQVGDEPPI LPDMGNRVTA LTQMESACVT
160 170 180 190 200
IHEALKTVID SQTHYRLREA QDRARAEDLN SRVSYWSVGE TIALFVVSFS
210 220
QVLLLKSFFT EKRPINRAVH S
Length:221
Mass (Da):25,510
Last modified:September 13, 2004 - v1
Checksum:i739E5B785791C443
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079220 mRNA. Translation: AAH79220.1.
RefSeqiNP_001004249.1. NM_001004249.1.
UniGeneiRn.98226.

Genome annotation databases

EnsembliENSRNOT00000018603; ENSRNOP00000018603; ENSRNOG00000013889.
GeneIDi300888.
KEGGirno:300888.
UCSCiRGD:1303327. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079220 mRNA. Translation: AAH79220.1.
RefSeqiNP_001004249.1. NM_001004249.1.
UniGeneiRn.98226.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018603.

PTM databases

iPTMnetiQ6AY25.
PhosphoSiteiQ6AY25.

Proteomic databases

PaxDbiQ6AY25.
PRIDEiQ6AY25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018603; ENSRNOP00000018603; ENSRNOG00000013889.
GeneIDi300888.
KEGGirno:300888.
UCSCiRGD:1303327. rat.

Organism-specific databases

CTDi23423.
RGDi1303327. Tmed3.

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ6AY25.
OMAiGRVSYWS.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ6AY25.
TreeFamiTF313000.

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

NextBioi647702.
PROiQ6AY25.

Gene expression databases

GenevisibleiQ6AY25. RN.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus."
    Barr F.A., Preisinger C., Kopajtich R., Koerner R.
    J. Cell Biol. 155:885-891(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH GORASP1 AND GORASP2.
  3. Cited for: METHYLATION AT ARG-103, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Golgin tethers define subpopulations of COPI vesicles."
    Malsam J., Satoh A., Pelletier L., Warren G.
    Science 307:1095-1098(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTMED3_RAT
AccessioniPrimary (citable) accession number: Q6AY25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.