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Protein

Transmembrane emp24 domain-containing protein 3

Gene

Tmed3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 3
Alternative name(s):
p24 family protein gamma-4
Short name:
p24gamma4
Gene namesi
Name:Tmed3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1303327. Tmed3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 184LumenalSequence analysisAdd BLAST154
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 221CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000001038631 – 221Transmembrane emp24 domain-containing protein 3Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103Dimethylated arginine1 Publication1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ6AY25.
PRIDEiQ6AY25.

PTM databases

iPTMnetiQ6AY25.
PhosphoSitePlusiQ6AY25.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013889.
GenevisibleiQ6AY25. RN.

Interactioni

Subunit structurei

Monomer in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED3; however, there are conflicting reports on the interaction (By similarity). Interacts with GORASP1 and GORASP2.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 124GOLDPROSITE-ProRule annotationAdd BLAST83

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 221COPI vesicle coat-bindingSequence analysisAdd BLAST14
Motifi208 – 209COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ6AY25.
KOiK20350.
OMAiGRVSYWS.
OrthoDBiEOG091G0LX8.
PhylomeDBiQ6AY25.
TreeFamiTF313000.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AY25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNEVPRASS FQMLMLLLLL LLLRAERLRG AELTFELPDN AKQCFHEEVE
60 70 80 90 100
QGVKFSLDYQ VITGGHYDVD CYVEDPMGNI IYRETKKQYD SFTYKTEVKG
110 120 130 140 150
VYRFCFSNEF STFSHKTVYF DFQVGDEPPI LPDMGNRVTA LTQMESACVT
160 170 180 190 200
IHEALKTVID SQTHYRLREA QDRARAEDLN SRVSYWSVGE TIALFVVSFS
210 220
QVLLLKSFFT EKRPINRAVH S
Length:221
Mass (Da):25,510
Last modified:September 13, 2004 - v1
Checksum:i739E5B785791C443
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079220 mRNA. Translation: AAH79220.1.
RefSeqiNP_001004249.1. NM_001004249.1.
UniGeneiRn.98226.

Genome annotation databases

EnsembliENSRNOT00000018603; ENSRNOP00000018603; ENSRNOG00000013889.
GeneIDi300888.
KEGGirno:300888.
UCSCiRGD:1303327. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079220 mRNA. Translation: AAH79220.1.
RefSeqiNP_001004249.1. NM_001004249.1.
UniGeneiRn.98226.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018603.

PTM databases

iPTMnetiQ6AY25.
PhosphoSitePlusiQ6AY25.

Proteomic databases

PaxDbiQ6AY25.
PRIDEiQ6AY25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018603; ENSRNOP00000018603; ENSRNOG00000013889.
GeneIDi300888.
KEGGirno:300888.
UCSCiRGD:1303327. rat.

Organism-specific databases

CTDi23423.
RGDi1303327. Tmed3.

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ6AY25.
KOiK20350.
OMAiGRVSYWS.
OrthoDBiEOG091G0LX8.
PhylomeDBiQ6AY25.
TreeFamiTF313000.

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ6AY25.

Gene expression databases

BgeeiENSRNOG00000013889.
GenevisibleiQ6AY25. RN.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMED3_RAT
AccessioniPrimary (citable) accession number: Q6AY25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: September 13, 2004
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.