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Protein

Atypical kinase COQ8B, mitochondrial

Gene

Coq8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Required for podocyte migration.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPBy similarity1
Binding sitei233ATPBy similarity1
Active sitei363Proton acceptorBy similarity1
Binding sitei368ATPBy similarity1
Binding sitei382ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi320 – 323ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase COQ8B, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
AarF domain-containing protein kinase 4Imported
Coenzyme Q protein 8BBy similarity
Gene namesi
Name:Coq8bBy similarity
Synonyms:Adck4Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1311356. Adck4.

Subcellular locationi

  • Mitochondrion membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasmcytosol By similarity
  • Cell membrane By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei93 – 109HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717991 – 528Atypical kinase COQ8B, mitochondrialAdd BLAST528

Proteomic databases

PaxDbiQ6AY19.
PRIDEiQ6AY19.

Expressioni

Tissue specificityi

In the kidney, expressed in glomeruli, predominantly in podocyte foot precesses, as well as in proximal tubules and collecting ducts (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000020848.
GenevisibleiQ6AY19. RN.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region. Interacts with COQ6 and COQ7. Interacts with the multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028290.

Structurei

3D structure databases

ProteinModelPortaliQ6AY19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 419Protein kinaseAdd BLAST233

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi151 – 154KxGQ motifBy similarity4
Motifi212 – 215AAAS motifBy similarity4

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains. The KxGQ motif completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ6AY19.
KOiK08869.
OMAiPCGGSWA.
OrthoDBiEOG091G02P9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

Q6AY19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLELGAMLR TTCGPLGRAV RLPCAGALRL RPHWWGPCRD CLAQRVHQDQ
60 70 80 90 100
PGRGLTEDEI RRAREARLRK APRPQLSDRS RERKVPASRI SRLASFGGLA
110 120 130 140 150
VGLGLGALAE VTKKSLPGGS LQHEGSSPFL TEANAERIVQ TLCTVRGAAL
160 170 180 190 200
KIGQMLSIQD NSLISPQLQR VFERVRQSAD FMPRWQMMKV LEEELGKDWQ
210 220 230 240 250
DKVASLEEVP FAAASIGQVH QGVLKDGTEV AVKIQYPGVA ESIQSDVQNL
260 270 280 290 300
LALLKMSVGL PEGLFAEQSL QTLQQELAWE CDYRREAACA QTFKKLLADD
310 320 330 340 350
PFFRVPAVVE ELCTTRVLGM ELAGGIPLDQ CQGLSQDIRN QICFQLLRLC
360 370 380 390 400
LRELFEFRFM QTDPNWANFL YDASSHKVTL LDFGASRAFG TEFTDHYIEV
410 420 430 440 450
VKAAADGDRD RVLQKSQDLK FLTGFETKAF SDAHVEAVMI LGEPFAASGS
460 470 480 490 500
YDFGAGETAR RIQGLIPVLL RHRLRPPPEE TYALHRKLAG AFLACARLHA
510 520
HIACRDLFQD TYHRYWASRQ TLPLPAAS
Length:528
Mass (Da):58,905
Last modified:September 13, 2004 - v1
Checksum:i6ECBC3DD231B16B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079227 mRNA. Translation: AAH79227.1.
RefSeqiNP_001012065.1. NM_001012065.1.
XP_017444608.1. XM_017589119.1.
UniGeneiRn.11549.

Genome annotation databases

EnsembliENSRNOT00000028290; ENSRNOP00000028290; ENSRNOG00000020848.
GeneIDi308453.
KEGGirno:308453.
UCSCiRGD:1311356. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079227 mRNA. Translation: AAH79227.1.
RefSeqiNP_001012065.1. NM_001012065.1.
XP_017444608.1. XM_017589119.1.
UniGeneiRn.11549.

3D structure databases

ProteinModelPortaliQ6AY19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028290.

Proteomic databases

PaxDbiQ6AY19.
PRIDEiQ6AY19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028290; ENSRNOP00000028290; ENSRNOG00000020848.
GeneIDi308453.
KEGGirno:308453.
UCSCiRGD:1311356. rat.

Organism-specific databases

CTDi308453.
RGDi1311356. Adck4.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ6AY19.
KOiK08869.
OMAiPCGGSWA.
OrthoDBiEOG091G02P9.

Miscellaneous databases

PROiQ6AY19.

Gene expression databases

BgeeiENSRNOG00000020848.
GenevisibleiQ6AY19. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ8B_RAT
AccessioniPrimary (citable) accession number: Q6AY19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.