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Protein

NADH-cytochrome b5 reductase 2

Gene

Cyb5r2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).HMP:

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADHMP:

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 137FADHMP:Add BLAST31
Nucleotide bindingi146 – 181FADHMP:Add BLAST36

GO - Molecular functioni

  • cytochrome-b5 reductase activity, acting on NAD(P)H Source: RGD
  • oxidoreductase activity Source: GO_Central

GO - Biological processi

  • oxidation-reduction process Source: RGD
  • sterol biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-RNO-1237044. Erythrocytes take up carbon dioxide and release oxygen.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 2 (EC:1.6.2.2)
Short name:
b5R.2
Gene namesi
Name:Cyb5r2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308421. Cyb5r2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875501 – 276NADH-cytochrome b5 reductase 2Add BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17N6-acetyllysineHMP:1
Modified residuei18PhosphotyrosineHMP:1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AY12.
PRIDEiQ6AY12.

PTM databases

PhosphoSitePlusiQ6AY12.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019751.
ExpressionAtlasiQ6AY12. baseline and differential.
GenevisibleiQ6AY12. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026744.

Structurei

3D structure databases

ProteinModelPortaliQ6AY12.
SMRiQ6AY12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 127FAD-binding FR-typeIEP:Add BLAST113

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.
GeneTreeiENSGT00390000008881.
HOGENOMiHOG000175005.
HOVERGENiHBG052580.
InParanoidiQ6AY12.
KOiK00326.
PhylomeDBiQ6AY12.

Family and domain databases

InterProiView protein in InterPro
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6AY12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKKKDLIT LQDPEAKYPL PLIEKEQINH NTRRFRFGLP SPDHVLGLPV
60 70 80 90 100
GNYVHLLAQI NNELVIRAYT PVSSDDDQGF VDLIIKIYFK NVHPKYPEGG
110 120 130 140 150
KMTQYLENMK IGDTILFRGP TGRLFYNEPG TLLIKTDKTS EPEKKLVHHL
160 170 180 190 200
GMIAGGTGIT PMLQLIRHIT KDTSDGTRMS LLFANQTEED ILLRKELEEV
210 220 230 240 250
ATTHQNQFSL WYTLDRPPSG WEYSSGFITA DMIKEHLPPP GEATLILVCG
260 270
PPPLIQEAAH PSLEQLGYTK DMIFTY
Length:276
Mass (Da):31,239
Last modified:September 13, 2004 - v1
Checksum:i2B44235766324E96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079235 mRNA. Translation: AAH79235.1.
RefSeqiNP_001014266.1. NM_001014244.1.
UniGeneiRn.16198.

Genome annotation databases

EnsembliENSRNOT00000026744; ENSRNOP00000026744; ENSRNOG00000019751.
GeneIDi365345.
KEGGirno:365345.
UCSCiRGD:1308421. rat.

Similar proteinsi

Entry informationi

Entry nameiNB5R2_RAT
AccessioniPrimary (citable) accession number: Q6AY12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: September 13, 2004
Last modified: November 22, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families