Reviewed, UniProtKB/Swiss-Prot Q6AY12 (NB5R2_RAT)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
      Short name=b5R.2
    EC=1.6.2.2

Gene names

Name:

Cyb5r2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NADH-cytochrome b5 reductase 2		PRO_0000287550
Regions
	Domain	15 – 127	113	FAD-binding FR-type
	Nucleotide binding	107 – 137	31	FAD By similarity
	Nucleotide binding	146 – 181	36	FAD By similarity
Amino acid modifications
	Modified residue	177	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AY12-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 2B44235766324E96	FASTA	276	31,239
	10 20 30 40 50 60 MSVKKKDLIT LQDPEAKYPL PLIEKEQINH NTRRFRFGLP SPDHVLGLPV GNYVHLLAQI 70 80 90 100 110 120 NNELVIRAYT PVSSDDDQGF VDLIIKIYFK NVHPKYPEGG KMTQYLENMK IGDTILFRGP 130 140 150 160 170 180 TGRLFYNEPG TLLIKTDKTS EPEKKLVHHL GMIAGGTGIT PMLQLIRHIT KDTSDGTRMS 190 200 210 220 230 240 LLFANQTEED ILLRKELEEV ATTHQNQFSL WYTLDRPPSG WEYSSGFITA DMIKEHLPPP 250 260 270 GEATLILVCG PPPLIQEAAH PSLEQLGYTK DMIFTY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079235 mRNA. Translation: AAH79235.1.
IPI	IPI00464515.
RefSeq	NP_001014266.1.
UniGene	Rn.16198
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6AY12.
Genome annotation databases
Ensembl	ENSRNOT00000026744; ENSRNOP00000026744; ENSRNOG00000019751; Rattus norvegicus. [Genome view]
GeneID	365345.
KEGG	rno:365345.
UCSC	NM_001014244. rat.
Organism-specific databases
CTD	365345.
RGD	1308421. Cyb5r2.
Phylogenomic databases
HOVERGEN	Q6AY12.
Enzyme and pathway databases
BRENDA	1.6.2.2. 248.
Gene expression databases
ArrayExpress	Q6AY12.
Genevestigator	Q6AY12.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	687212.

Entry information

Entry name

NB5R2_RAT

Accession

Primary (citable) accession number: Q6AY12

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	September 13, 2004
Last modified:	October 13, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)