Q6AXW7 (DUS18_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 18
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:

Dusp18

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment By similarity. Ref.2
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane Nucleus
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	integral to mitochondrial inner membrane Inferred from direct assay Ref.2. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Dual specificity protein phosphatase 18		PRO_0000094831
Regions
	Domain	80 – 149	70	Tyrosine-protein phosphatase
	Region	95 – 141	47	Sufficient for mitochondrial localization By similarity
Sites
	Active site	104	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AXW7 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 1A5BBC759522E442	FASTA	204	22,754
	10 20 30 40 50 60 MTSPWSACPV QFPQPSISGL SQITKSLFIS NGAAANDKLL LSSNQITTVI NVSVEVANTF 70 80 90 100 110 120 YEDIQYVQVP VVDAPIARLS DFFDPIADHI HSVEMKQGRT LLHCAAGVSR SAALCLAYLM 130 140 150 160 170 180 KYHVMSLLDA HAWTKSRRPI IRPNSGFWEQ LIHYEFQLFG KNTMQMVNSP MGLIPDIYEK 190 200 ETRMMIPLST PDGAFHKGKE KALL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane." Rardin M.J., Wiley S.E., Murphy A.N., Pagliarini D.J., Dixon J.E. J. Biol. Chem. 283:15440-15450(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079285 mRNA. Translation: AAH79285.1.
IPI	IPI00464600.
RefSeq	NP_001013146.1. NM_001013128.1.
UniGene	Rn.19584.
3D structure databases
ProteinModelPortal	Q6AXW7.
SMR	Q6AXW7. Positions 18-179.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000005516.
PTM databases
PhosphoSite	Q6AXW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	305477.
KEGG	rno:305477.
UCSC	RGD:1306929. rat.
Organism-specific databases
CTD	150290.
RGD	1306929. Dusp18.
Phylogenomic databases
eggNOG	COG2453.
HOGENOM	HOG000233766.
HOVERGEN	HBG051422.
InParanoid	Q6AXW7.
KO	K14165.
OrthoDB	EOG4VQ9QB.
Gene expression databases
ArrayExpress	Q6AXW7.
Genevestigator	Q6AXW7.
GermOnline	ENSRNOG00000024945. Rattus norvegicus.
Family and domain databases
InterPro	IPR020417. Atypical_DUSP. IPR020420. Atypical_DUSP_famB. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PRINTS	PR01908. ADSPHPHTASE. PR01910. ADSPHPHTASEB.
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	654674.

Entry information

Entry name

DUS18_RAT

Accession

Primary (citable) accession number: Q6AXW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	September 13, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program