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Protein

Dual specificity protein phosphatase 18

Gene

Dusp18

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. peptidyl-tyrosine dephosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 18 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:Dusp18
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1306929. Dusp18.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication
Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment.By similarity

GO - Cellular componenti

  1. extrinsic component of mitochondrial inner membrane Source: RGD
  2. mitochondrial intermembrane space Source: RGD
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Dual specificity protein phosphatase 18PRO_0000094831Add
BLAST

PTM databases

PhosphoSiteiQ6AXW7.

Expressioni

Gene expression databases

GenevestigatoriQ6AXW7.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005516.

Structurei

3D structure databases

ProteinModelPortaliQ6AXW7.
SMRiQ6AXW7. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 14970Tyrosine-protein phosphataseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 14147Sufficient for mitochondrial localizationBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ6AXW7.
KOiK14165.
PhylomeDBiQ6AXW7.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AXW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPWSACPV QFPQPSISGL SQITKSLFIS NGAAANDKLL LSSNQITTVI
60 70 80 90 100
NVSVEVANTF YEDIQYVQVP VVDAPIARLS DFFDPIADHI HSVEMKQGRT
110 120 130 140 150
LLHCAAGVSR SAALCLAYLM KYHVMSLLDA HAWTKSRRPI IRPNSGFWEQ
160 170 180 190 200
LIHYEFQLFG KNTMQMVNSP MGLIPDIYEK ETRMMIPLST PDGAFHKGKE

KALL
Length:204
Mass (Da):22,754
Last modified:September 13, 2004 - v1
Checksum:i1A5BBC759522E442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079285 mRNA. Translation: AAH79285.1.
RefSeqiNP_001013146.2. NM_001013128.2.
UniGeneiRn.19584.

Genome annotation databases

GeneIDi305477.
KEGGirno:305477.
UCSCiRGD:1306929. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079285 mRNA. Translation: AAH79285.1.
RefSeqiNP_001013146.2. NM_001013128.2.
UniGeneiRn.19584.

3D structure databases

ProteinModelPortaliQ6AXW7.
SMRiQ6AXW7. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005516.

PTM databases

PhosphoSiteiQ6AXW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi305477.
KEGGirno:305477.
UCSCiRGD:1306929. rat.

Organism-specific databases

CTDi150290.
RGDi1306929. Dusp18.

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ6AXW7.
KOiK14165.
PhylomeDBiQ6AXW7.

Miscellaneous databases

NextBioi654674.
PROiQ6AXW7.

Gene expression databases

GenevestigatoriQ6AXW7.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane."
    Rardin M.J., Wiley S.E., Murphy A.N., Pagliarini D.J., Dixon J.E.
    J. Biol. Chem. 283:15440-15450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUS18_RAT
AccessioniPrimary (citable) accession number: Q6AXW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: September 13, 2004
Last modified: February 4, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.