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Protein

Borealin

Gene

Cdca8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Borealin
Alternative name(s):
Cell division cycle-associated protein 8
Gene namesi
Name:Cdca8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1566306. Cdca8.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Cytoplasm By similarity
  • Chromosomecentromere By similarity
  • Cytoplasmcytoskeletonspindle By similarity

  • Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470781 – 288BorealinAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88Phosphothreonine; by TTKBy similarity1
Modified residuei91CitrullineBy similarity1
Modified residuei94Phosphothreonine; by TTKBy similarity1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei110PhosphoserineBy similarity1
Modified residuei174Phosphoserine; by AURKBBy similarity1
Modified residuei197PhosphothreonineBy similarity1
Modified residuei212PhosphothreonineBy similarity1
Modified residuei227PhosphoserineCombined sources1
Modified residuei232PhosphoserineBy similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei252PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by TTK, essentially at Thr-88 and Thr-94. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.By similarity
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3 (By similarity).By similarity
Citrullinated by PADI4.By similarity

Keywords - PTMi

Citrullination, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AXW0.
PRIDEiQ6AXW0.

PTM databases

iPTMnetiQ6AXW0.
PhosphoSitePlusiQ6AXW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ6AXW0. baseline and differential.
GenevisibleiQ6AXW0. RN.

Interactioni

Subunit structurei

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5. Interacts with SENP3, UBE2I and RANBP2. Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043633.

Structurei

3D structure databases

ProteinModelPortaliQ6AXW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149Required for interaction with SENP3By similarityAdd BLAST149
Regioni1 – 88Required for centromere localizationBy similarityAdd BLAST88
Regioni1 – 58Required for interaction with INCENPBy similarityAdd BLAST58
Regioni10 – 109Required to form a minimal CPC core complex that localizes to the central spindle and midbody and properly executes the role of the CPC during cytokinesisBy similarityAdd BLAST100
Regioni20 – 78Required for interaction with INCENP and BIRC5By similarityAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi125 – 133Poly-Glu9
Compositional biasi134 – 139Poly-Gly6

Domaini

The C-terminal region (aa 215-288) represents the dimerization motif.By similarity

Sequence similaritiesi

Belongs to the borealin family.Curated

Phylogenomic databases

eggNOGiENOG410IICJ. Eukaryota.
ENOG4111N8R. LUCA.
GeneTreeiENSGT00390000011115.
HOVERGENiHBG080103.
InParanoidiQ6AXW0.
KOiK11514.
OMAiQIESDRQ.
OrthoDBiEOG091G167R.
PhylomeDBiQ6AXW0.

Family and domain databases

InterProiIPR018851. Borealin_N.
IPR018867. Cell_div_borealin.
[Graphical view]
PfamiPF10512. Borealin. 1 hit.
PF10444. Nbl1_Borealin_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AXW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKRSSRG TRTNTLRSRK LASFLKDFDR EVQVRTKQIE SDRQTLLKEV
60 70 80 90 100
ENLYNIEVLR LPKALQVMKW LDYFALGGNR QALEEAATAD RDITEINNLT
110 120 130 140 150
AEAIQTPLKS VKKRKVIEVD EAIKEEEEDE EEEGGGGGGR KSHKNLRSAR
160 170 180 190 200
VKRCPPSKKR TQSIQGRSRS KRLSHDFVTP AMSRLEPSLV KPTPGMTPRF
210 220 230 240 250
DSRVFKTPGL RTPAAKEQVY NISINGSPLA DSKEISLSVP IGGGASLRLL
260 270 280
ASDLQRVDIA QLNPEALGNI KKLSSRLAQI CSSIRTGR
Length:288
Mass (Da):32,099
Last modified:September 13, 2004 - v1
Checksum:i1CF10957DF7635CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079293 mRNA. Translation: AAH79293.1.
BC079274 mRNA. Translation: AAH79274.1.
RefSeqiNP_001020221.1. NM_001025050.1.
XP_008762286.1. XM_008764064.1.
UniGeneiRn.14970.

Genome annotation databases

EnsembliENSRNOT00000048293; ENSRNOP00000043633; ENSRNOG00000031431.
GeneIDi500545.
KEGGirno:500545.
UCSCiRGD:1566306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079293 mRNA. Translation: AAH79293.1.
BC079274 mRNA. Translation: AAH79274.1.
RefSeqiNP_001020221.1. NM_001025050.1.
XP_008762286.1. XM_008764064.1.
UniGeneiRn.14970.

3D structure databases

ProteinModelPortaliQ6AXW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043633.

PTM databases

iPTMnetiQ6AXW0.
PhosphoSitePlusiQ6AXW0.

Proteomic databases

PaxDbiQ6AXW0.
PRIDEiQ6AXW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000048293; ENSRNOP00000043633; ENSRNOG00000031431.
GeneIDi500545.
KEGGirno:500545.
UCSCiRGD:1566306. rat.

Organism-specific databases

CTDi55143.
RGDi1566306. Cdca8.

Phylogenomic databases

eggNOGiENOG410IICJ. Eukaryota.
ENOG4111N8R. LUCA.
GeneTreeiENSGT00390000011115.
HOVERGENiHBG080103.
InParanoidiQ6AXW0.
KOiK11514.
OMAiQIESDRQ.
OrthoDBiEOG091G167R.
PhylomeDBiQ6AXW0.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ6AXW0.

Gene expression databases

ExpressionAtlasiQ6AXW0. baseline and differential.
GenevisibleiQ6AXW0. RN.

Family and domain databases

InterProiIPR018851. Borealin_N.
IPR018867. Cell_div_borealin.
[Graphical view]
PfamiPF10512. Borealin. 1 hit.
PF10444. Nbl1_Borealin_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBOREA_RAT
AccessioniPrimary (citable) accession number: Q6AXW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.