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Protein

Borealin

Gene

Cdca8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2500257 Resolution of Sister Chromatid Cohesion
R-RNO-4615885 SUMOylation of DNA replication proteins
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Borealin
Alternative name(s):
Cell division cycle-associated protein 8
Gene namesi
Name:Cdca8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1566306 Cdca8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470781 – 288BorealinAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88Phosphothreonine; by TTKBy similarity1
Modified residuei91CitrullineBy similarity1
Modified residuei94Phosphothreonine; by TTKBy similarity1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei110PhosphoserineBy similarity1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei174Phosphoserine; by AURKBBy similarity1
Modified residuei197PhosphothreonineBy similarity1
Modified residuei212PhosphothreonineBy similarity1
Modified residuei227PhosphoserineCombined sources1
Modified residuei232PhosphoserineBy similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei252PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by TTK, essentially at Thr-88 and Thr-94. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.By similarity
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3 (By similarity).By similarity
Citrullinated by PADI4.By similarity

Keywords - PTMi

Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AXW0
PRIDEiQ6AXW0

PTM databases

iPTMnetiQ6AXW0
PhosphoSitePlusiQ6AXW0

Expressioni

Gene expression databases

BgeeiENSRNOG00000031431
GenevisibleiQ6AXW0 RN

Interactioni

Subunit structurei

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5. Interacts with SENP3, UBE2I and RANBP2. Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043633

Structurei

3D structure databases

ProteinModelPortaliQ6AXW0
SMRiQ6AXW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149Required for interaction with SENP3By similarityAdd BLAST149
Regioni1 – 88Required for centromere localizationBy similarityAdd BLAST88
Regioni1 – 58Required for interaction with INCENPBy similarityAdd BLAST58
Regioni10 – 109Required to form a minimal CPC core complex that localizes to the central spindle and midbody and properly executes the role of the CPC during cytokinesisBy similarityAdd BLAST100
Regioni20 – 78Required for interaction with INCENP and BIRC5By similarityAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi125 – 133Poly-Glu9
Compositional biasi134 – 139Poly-Gly6

Domaini

The C-terminal region (aa 215-288) represents the dimerization motif.By similarity

Sequence similaritiesi

Belongs to the borealin family.Curated

Phylogenomic databases

eggNOGiENOG410IICJ Eukaryota
ENOG4111N8R LUCA
GeneTreeiENSGT00390000011115
HOVERGENiHBG080103
InParanoidiQ6AXW0
KOiK11514
OMAiQIESDRQ
OrthoDBiEOG091G167R
PhylomeDBiQ6AXW0

Family and domain databases

InterProiView protein in InterPro
IPR018851 Borealin_N
IPR018867 Cell_div_borealin
PfamiView protein in Pfam
PF10512 Borealin, 1 hit
PF10444 Nbl1_Borealin_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AXW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKRSSRG TRTNTLRSRK LASFLKDFDR EVQVRTKQIE SDRQTLLKEV
60 70 80 90 100
ENLYNIEVLR LPKALQVMKW LDYFALGGNR QALEEAATAD RDITEINNLT
110 120 130 140 150
AEAIQTPLKS VKKRKVIEVD EAIKEEEEDE EEEGGGGGGR KSHKNLRSAR
160 170 180 190 200
VKRCPPSKKR TQSIQGRSRS KRLSHDFVTP AMSRLEPSLV KPTPGMTPRF
210 220 230 240 250
DSRVFKTPGL RTPAAKEQVY NISINGSPLA DSKEISLSVP IGGGASLRLL
260 270 280
ASDLQRVDIA QLNPEALGNI KKLSSRLAQI CSSIRTGR
Length:288
Mass (Da):32,099
Last modified:September 13, 2004 - v1
Checksum:i1CF10957DF7635CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079293 mRNA Translation: AAH79293.1
BC079274 mRNA Translation: AAH79274.1
RefSeqiNP_001020221.1, NM_001025050.1
XP_008762286.1, XM_008764064.1
UniGeneiRn.14970

Genome annotation databases

EnsembliENSRNOT00000048293; ENSRNOP00000043633; ENSRNOG00000031431
GeneIDi500545
KEGGirno:500545
UCSCiRGD:1566306 rat

Similar proteinsi

Entry informationi

Entry nameiBOREA_RAT
AccessioniPrimary (citable) accession number: Q6AXW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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