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Protein

Borealin

Gene

Cdca8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Borealin
Alternative name(s):
Cell division cycle-associated protein 8
Gene namesi
Name:Cdca8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1566306. Cdca8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288BorealinPRO_0000247078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881Phosphothreonine; by TTKBy similarity
Modified residuei91 – 911CitrullineBy similarity
Modified residuei94 – 941Phosphothreonine; by TTKBy similarity
Modified residuei106 – 1061PhosphothreonineBy similarity
Modified residuei110 – 1101PhosphoserineBy similarity
Modified residuei174 – 1741Phosphoserine; by AURKBBy similarity
Modified residuei197 – 1971PhosphothreonineBy similarity
Modified residuei212 – 2121PhosphothreonineBy similarity
Modified residuei227 – 2271PhosphoserineCombined sources
Modified residuei232 – 2321PhosphoserineBy similarity
Modified residuei246 – 2461PhosphoserineBy similarity
Modified residuei252 – 2521PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by TTK, essentially at Thr-88 and Thr-94.By similarity
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3 (By similarity).By similarity
Citrullinated by PADI4.By similarity

Keywords - PTMi

Citrullination, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AXW0.
PRIDEiQ6AXW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ6AXW0. baseline and differential.
GenevisibleiQ6AXW0. RN.

Interactioni

Subunit structurei

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC (By similarity). Interacts with BIRC5/survivin and INCENP; interaction is direct. Interacts with SENP3, UBE2I and RANBP2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043633.

Structurei

3D structure databases

ProteinModelPortaliQ6AXW0.
SMRiQ6AXW0. Positions 15-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 149149Required for interaction with SENP3By similarityAdd
BLAST
Regioni1 – 8888Required for centromere localizationBy similarityAdd
BLAST
Regioni1 – 5858Required for interaction with INCENPBy similarityAdd
BLAST
Regioni10 – 109100Required to form a minimal CPC core complex that localizes to the central spindle and midbody and properly executes the role of the CPC during cytokinesisBy similarityAdd
BLAST
Regioni20 – 7859Required for interaction with INCENP and BIRC5By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi125 – 1339Poly-Glu
Compositional biasi134 – 1396Poly-Gly

Domaini

The C-terminal region (aa 215-288) represents the dimerization motif.By similarity

Sequence similaritiesi

Belongs to the borealin family.Curated

Phylogenomic databases

eggNOGiENOG410IICJ. Eukaryota.
ENOG4111N8R. LUCA.
GeneTreeiENSGT00390000011115.
HOVERGENiHBG080103.
InParanoidiQ6AXW0.
KOiK11514.
PhylomeDBiQ6AXW0.

Family and domain databases

InterProiIPR018851. Borealin_N.
IPR018867. Cell_div_borealin.
[Graphical view]
PfamiPF10512. Borealin. 1 hit.
PF10444. Nbl1_Borealin_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6AXW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKRSSRG TRTNTLRSRK LASFLKDFDR EVQVRTKQIE SDRQTLLKEV
60 70 80 90 100
ENLYNIEVLR LPKALQVMKW LDYFALGGNR QALEEAATAD RDITEINNLT
110 120 130 140 150
AEAIQTPLKS VKKRKVIEVD EAIKEEEEDE EEEGGGGGGR KSHKNLRSAR
160 170 180 190 200
VKRCPPSKKR TQSIQGRSRS KRLSHDFVTP AMSRLEPSLV KPTPGMTPRF
210 220 230 240 250
DSRVFKTPGL RTPAAKEQVY NISINGSPLA DSKEISLSVP IGGGASLRLL
260 270 280
ASDLQRVDIA QLNPEALGNI KKLSSRLAQI CSSIRTGR
Length:288
Mass (Da):32,099
Last modified:September 13, 2004 - v1
Checksum:i1CF10957DF7635CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079293 mRNA. Translation: AAH79293.1.
BC079274 mRNA. Translation: AAH79274.1.
RefSeqiNP_001020221.1. NM_001025050.1.
XP_008762286.1. XM_008764064.1.
UniGeneiRn.14970.

Genome annotation databases

EnsembliENSRNOT00000048293; ENSRNOP00000043633; ENSRNOG00000031431.
GeneIDi500545.
KEGGirno:500545.
UCSCiRGD:1566306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079293 mRNA. Translation: AAH79293.1.
BC079274 mRNA. Translation: AAH79274.1.
RefSeqiNP_001020221.1. NM_001025050.1.
XP_008762286.1. XM_008764064.1.
UniGeneiRn.14970.

3D structure databases

ProteinModelPortaliQ6AXW0.
SMRiQ6AXW0. Positions 15-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043633.

Proteomic databases

PaxDbiQ6AXW0.
PRIDEiQ6AXW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000048293; ENSRNOP00000043633; ENSRNOG00000031431.
GeneIDi500545.
KEGGirno:500545.
UCSCiRGD:1566306. rat.

Organism-specific databases

CTDi55143.
RGDi1566306. Cdca8.

Phylogenomic databases

eggNOGiENOG410IICJ. Eukaryota.
ENOG4111N8R. LUCA.
GeneTreeiENSGT00390000011115.
HOVERGENiHBG080103.
InParanoidiQ6AXW0.
KOiK11514.
PhylomeDBiQ6AXW0.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-4615885. SUMOylation of DNA replication proteins.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

NextBioi706493.
PROiQ6AXW0.

Gene expression databases

ExpressionAtlasiQ6AXW0. baseline and differential.
GenevisibleiQ6AXW0. RN.

Family and domain databases

InterProiIPR018851. Borealin_N.
IPR018867. Cell_div_borealin.
[Graphical view]
PfamiPF10512. Borealin. 1 hit.
PF10444. Nbl1_Borealin_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBOREA_RAT
AccessioniPrimary (citable) accession number: Q6AXW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: September 13, 2004
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.