Q6AXV9 (GSTO2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase omega-2
Short name=GSTO-2
EC=2.5.1.18

Alternative name(s):
Glutathione S-transferase omega 2-2
Short name=GSTO 2-2
Glutathione-dependent dehydroascorbate reductase
EC=1.8.5.1
Monomethylarsonic acid reductase
Short name=MMA(V) reductase
EC=1.20.4.2

Gene names

Name:

Gsto2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activity. Has high dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione. 2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.
Sequence similarities	Belongs to the GST superfamily. Omega family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-ascorbic acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular response to arsenic-containing substance Inferred from sequence or structural similarity. Source: UniProtKB xenobiotic metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	glutathione dehydrogenase (ascorbate) activity Inferred from sequence or structural similarity. Source: UniProtKB glutathione transferase activity Inferred from electronic annotation. Source: EC methylarsonate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Glutathione S-transferase omega-2		PRO_0000239142
Regions
	Domain	22 – 101	80	GST N-terminal
	Domain	106 – 231	126	GST C-terminal
	Region	85 – 86	2	Glutathione binding By similarity
Sites
	Active site	32	1	Nucleophile By similarity
	Binding site	59	1	Glutathione By similarity
	Binding site	72	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6AXV9 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: 2DD551FC7CF1EE47	FASTA	248	28,679
	10 20 30 40 50 60 MSGDLTRCLG KGSCPPGPVP EGVIRIYSMR FCPYSHRTRL VLKAKSIRHE IININLKNKP 70 80 90 100 110 120 DWYYTKHPFG QVPVLENSQC QLIYESVIAC EYLDDVFPGR KLFPYDPYER ARQKMLLELF 130 140 150 160 170 180 CKVPQLSKEC LVALRCGRDC TDLKVALRQE LCNLEEILEY QNTTFFGGDS ISMIDYLVWP 190 200 210 220 230 240 WFERLDVYGL ADCVNHTPML RLWISSMKQD PAVCALHIDK NIFLGFLNLY FQNNPCAFDF GLCGPIVR

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079295 mRNA. Translation: AAH79295.1.
IPI	IPI00361569.
RefSeq	NP_001012071.1. NM_001012071.1. XP_003749186.1. XM_003749138.1.
UniGene	Rn.206114.
3D structure databases
ProteinModelPortal	Q6AXV9.
SMR	Q6AXV9. Positions 7-242.
ModBase	Search...
Proteomic databases
PaxDb	Q6AXV9.
PRIDE	Q6AXV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017186; ENSRNOP00000017186; ENSRNOG00000012801. ENSRNOT00000064023; ENSRNOP00000060669; ENSRNOG00000049964.
GeneID	100909560. 309465.
KEGG	rno:100909560. rno:309465.
UCSC	RGD:1310764. rat.
Organism-specific databases
CTD	119391.
RGD	1310764. Gsto2.
Phylogenomic databases
eggNOG	COG0625.
GeneTree	ENSGT00390000005479.
HOGENOM	HOG000006560.
HOVERGEN	HBG051853.
InParanoid	Q6AXV9.
KO	K00799.
OrthoDB	EOG43TZW5.
Gene expression databases
ArrayExpress	Q6AXV9.
Genevestigator	Q6AXV9.
GermOnline	ENSRNOG00000012801. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR005442. GST_omega. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
PRINTS	PR01625. GSTRNSFRASEO.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	660856.

Entry information

Entry name

GSTO2_RAT

Accession

Primary (citable) accession number: Q6AXV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 13, 2004
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program