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Protein

Bactericidal permeability-increasing protein

Gene

Bpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.By similarity

GO - Molecular functioni

  • lipid binding Source: RGD
  • lipopolysaccharide binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial

Enzyme and pathway databases

ReactomeiR-RNO-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Bactericidal permeability-increasing protein
Short name:
BPI
Gene namesi
Name:Bpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1303179. Bpi.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000035833328 – 482Bactericidal permeability-increasing proteinAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi161 ↔ 201By similarity
Glycosylationi375N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6AXU0.
PRIDEiQ6AXU0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000034195.

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049523.

Structurei

3D structure databases

ProteinModelPortaliQ6AXU0.
SMRiQ6AXU0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 188N-terminal barrelBy similarityAdd BLAST183
Regioni189 – 254Central sheetBy similarityAdd BLAST66
Regioni235 – 240Cleavage sites for elastaseBy similarity6
Regioni255 – 425C-terminal barrelBy similarityAdd BLAST171

Domaini

The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested (By similarity).By similarity
The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4160. Eukaryota.
ENOG410Z88E. LUCA.
GeneTreeiENSGT00730000110583.
HOGENOMiHOG000231250.
HOVERGENiHBG002797.
InParanoidiQ6AXU0.
OMAiGWLIQLF.
OrthoDBiEOG091G08NV.
PhylomeDBiQ6AXU0.
TreeFamiTF315617.

Family and domain databases

InterProiView protein in InterPro
IPR017943. Bactericidal_perm-incr_a/b_dom.
IPR030181. BPI.
IPR030675. BPI/LBP.
IPR032942. BPI/LBP/Plunc.
IPR001124. Lipid-bd_serum_glycop_C.
IPR017942. Lipid-bd_serum_glycop_N.
PANTHERiPTHR10504. PTHR10504. 1 hit.
PTHR10504:SF109. PTHR10504:SF109. 1 hit.
PfamiView protein in Pfam
PF01273. LBP_BPI_CETP. 1 hit.
PF02886. LBP_BPI_CETP_C. 1 hit.
PIRSFiPIRSF002417. Lipid_binding_protein. 1 hit.
SMARTiView protein in SMART
SM00328. BPI1. 1 hit.
SM00329. BPI2. 1 hit.
SUPFAMiSSF55394. SSF55394. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AXU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWGPDNVRK WSSLALLAIV GTALTAATDP GFVARISQKG LDFVCQESMV
60 70 80 90 100
ELQKELLAIS IPDFSGDFKI KHLGKGTYEF YSMAVEGFHI PDPQIKLLPS
110 120 130 140 150
DGLQLSITSA SIKISGRWKY RKNILKASGN FQLSIQGVSI IADLILGNDP
160 170 180 190 200
SGRITITCST CDSHINSVRI KVSGSMLGWL IQLFHRKIET SLKKTIYKKI
210 220 230 240 250
CKIVRNSVSA KLQPYVKTLP VVAKVDDITS IDYSLLAPPM TTDKFLEGQL
260 270 280 290 300
RGEFFWRGHH GPFPAVPPVM NILPNNNYMV CMGISDYFFN TAEFAYQESE
310 320 330 340 350
TLKITLRDQL LAKDARYHLN TDFLKTFLPE VAKKFPSMGL QLLISAPLFA
360 370 380 390 400
HLNIQPSGLS LSPNLETRAF VVLPNSSLIP LFLLGMKTNA SLEVNAMKNR
410 420 430 440 450
LIGEMKLGRL LLELKQSNFG SFKVELLEDV INYLMSTMVL PKINEKLRRG
460 470 480
FPLPLPAGIQ LINSILYSSQ NFLLLEADLH RT
Length:482
Mass (Da):53,752
Last modified:September 13, 2004 - v1
Checksum:i269591C4C2F5A6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079318 mRNA. Translation: AAH79318.1.
RefSeqiNP_001004079.1. NM_001004079.1.
UniGeneiRn.109395.

Genome annotation databases

EnsembliENSRNOT00000039994; ENSRNOP00000049523; ENSRNOG00000034195.
GeneIDi296321.
KEGGirno:296321.

Similar proteinsi

Entry informationi

Entry nameiBPI_RAT
AccessioniPrimary (citable) accession number: Q6AXU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: September 13, 2004
Last modified: September 27, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families