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Protein

Bactericidal permeability-increasing protein

Gene

Bpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.By similarity

GO - Molecular functioni

  • lipid binding Source: RGD
  • lipopolysaccharide binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Enzyme and pathway databases

ReactomeiR-RNO-166016. Toll Like Receptor 4 (TLR4) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Bactericidal permeability-increasing protein
Short name:
BPI
Gene namesi
Name:Bpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1303179. Bpi.

Subcellular locationi

  • Secreted By similarity
  • Cytoplasmic granule membrane By similarity

  • Note: Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: InterPro
  • membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 482455Bactericidal permeability-increasing proteinPRO_0000358333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi161 ↔ 201By similarity
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6AXU0.
PRIDEiQ6AXU0.

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049523.

Structurei

3D structure databases

ProteinModelPortaliQ6AXU0.
SMRiQ6AXU0. Positions 29-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 188183N-terminal barrel By similarityBy similarityAdd
BLAST
Regioni189 – 25466Central sheet By similarityBy similarityAdd
BLAST
Regioni235 – 2406Cleavage sites for elastaseBy similarity
Regioni255 – 425171C-terminal barrel By similarityBy similarityAdd
BLAST

Domaini

The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested (By similarity).By similarity
The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4160. Eukaryota.
ENOG410Z88E. LUCA.
GeneTreeiENSGT00730000110583.
HOGENOMiHOG000231250.
HOVERGENiHBG002797.
InParanoidiQ6AXU0.
OMAiELKHSDV.
OrthoDBiEOG76739B.
PhylomeDBiQ6AXU0.
TreeFamiTF315617.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR030181. BPI.
IPR030675. BPI/LBP.
IPR032942. BPI/LBP/Plunc.
IPR001124. Lipid-bd_serum_glycop_C.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 1 hit.
PTHR10504:SF84. PTHR10504:SF84. 1 hit.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
PF02886. LBP_BPI_CETP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002417. Lipid_binding_protein. 1 hit.
SMARTiSM00328. BPI1. 1 hit.
SM00329. BPI2. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AXU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWGPDNVRK WSSLALLAIV GTALTAATDP GFVARISQKG LDFVCQESMV
60 70 80 90 100
ELQKELLAIS IPDFSGDFKI KHLGKGTYEF YSMAVEGFHI PDPQIKLLPS
110 120 130 140 150
DGLQLSITSA SIKISGRWKY RKNILKASGN FQLSIQGVSI IADLILGNDP
160 170 180 190 200
SGRITITCST CDSHINSVRI KVSGSMLGWL IQLFHRKIET SLKKTIYKKI
210 220 230 240 250
CKIVRNSVSA KLQPYVKTLP VVAKVDDITS IDYSLLAPPM TTDKFLEGQL
260 270 280 290 300
RGEFFWRGHH GPFPAVPPVM NILPNNNYMV CMGISDYFFN TAEFAYQESE
310 320 330 340 350
TLKITLRDQL LAKDARYHLN TDFLKTFLPE VAKKFPSMGL QLLISAPLFA
360 370 380 390 400
HLNIQPSGLS LSPNLETRAF VVLPNSSLIP LFLLGMKTNA SLEVNAMKNR
410 420 430 440 450
LIGEMKLGRL LLELKQSNFG SFKVELLEDV INYLMSTMVL PKINEKLRRG
460 470 480
FPLPLPAGIQ LINSILYSSQ NFLLLEADLH RT
Length:482
Mass (Da):53,752
Last modified:September 13, 2004 - v1
Checksum:i269591C4C2F5A6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079318 mRNA. Translation: AAH79318.1.
RefSeqiNP_001004079.1. NM_001004079.1.
UniGeneiRn.109395.

Genome annotation databases

EnsembliENSRNOT00000039994; ENSRNOP00000049523; ENSRNOG00000034195.
GeneIDi296321.
KEGGirno:296321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079318 mRNA. Translation: AAH79318.1.
RefSeqiNP_001004079.1. NM_001004079.1.
UniGeneiRn.109395.

3D structure databases

ProteinModelPortaliQ6AXU0.
SMRiQ6AXU0. Positions 29-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049523.

Proteomic databases

PaxDbiQ6AXU0.
PRIDEiQ6AXU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000039994; ENSRNOP00000049523; ENSRNOG00000034195.
GeneIDi296321.
KEGGirno:296321.

Organism-specific databases

CTDi671.
RGDi1303179. Bpi.

Phylogenomic databases

eggNOGiKOG4160. Eukaryota.
ENOG410Z88E. LUCA.
GeneTreeiENSGT00730000110583.
HOGENOMiHOG000231250.
HOVERGENiHBG002797.
InParanoidiQ6AXU0.
OMAiELKHSDV.
OrthoDBiEOG76739B.
PhylomeDBiQ6AXU0.
TreeFamiTF315617.

Enzyme and pathway databases

ReactomeiR-RNO-166016. Toll Like Receptor 4 (TLR4) Cascade.

Miscellaneous databases

NextBioi641019.
PROiQ6AXU0.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR030181. BPI.
IPR030675. BPI/LBP.
IPR032942. BPI/LBP/Plunc.
IPR001124. Lipid-bd_serum_glycop_C.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 1 hit.
PTHR10504:SF84. PTHR10504:SF84. 1 hit.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
PF02886. LBP_BPI_CETP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002417. Lipid_binding_protein. 1 hit.
SMARTiSM00328. BPI1. 1 hit.
SM00329. BPI2. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiBPI_RAT
AccessioniPrimary (citable) accession number: Q6AXU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: September 13, 2004
Last modified: March 16, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.